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Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization.

A correction to this paper has been published: the notice, 42744926, from Europe PMC.

Overview

Authors: Margot Sahnine1, Morgane Crochet1, Stéphane Tachon2, Sylvie Goussard1, Esthel Pénard3, Cyril Scandola3, Magalie Duchateau4, Quentin Giai Gianetto4,5, Audrey Salles6, Gaël Moneron7, Léa Swistak8, Arthur Charles-Orszag1,9, Adeline Mallet3, Mariette Matondo4, Jean-Yves Tinevez10, Daria Bonazzi1, Anna Sartori-Rupp2, Guillaume Duménil1, Dorian Obino1
ORCID iDs: Stéphane Tachon, Sylvie Goussard, Esthel Pénard, Cyril Scandola, Audrey Salles, Gaël Moneron, Léa Swistak, Arthur Charles-Orszag, Mariette Matondo, Anna Sartori-Rupp, Guillaume Duménil, Dorian Obino
  1. Institut Pasteur, Université Paris Cité, INSERM U1225, Pathogenesis of Vascular Infections Unit,F-75015 Paris, France
  2. Institut Pasteur, Université Paris Cité, NanoImaging Core Facility,F-75015 Paris, France
  3. Institut Pasteur, Université Paris Cité, Ultrastructural BioImaging Core Facility,F-75015 Paris, France
  4. Institut Pasteur, Université Paris Cité, Proteomics Core Facility, Mass Spectrometry for Biology Unit, UAR CNRS 2024,F-75015 Paris, France
  5. Institut Pasteur, Université Paris Cité, Bioinformatics and Biostatistics HUB,F-75015 Paris, France
  6. Institut Pasteur, Université Paris Cité, Photonic Bio-Imaging Unit, Centre de Ressources et Recherches Technologiques (UTechS-PBI, C2RT),F-75015 Paris, France
  7. Institut Pasteur, Université Paris Cité, CNRS UMR3571, Signaling and Receptors Dynamics Unit,F-75015 Paris, France
  8. Institut Pasteur, Université Paris Cité, CNRS UMR3691, Dynamics of Host-Pathogen Interactions Unit,F-75015 Paris, France
  9. Present Address: Department of Microbiology and Molecular Genetics, University of California,Davis, CA 95616 USA
  10. Institut Pasteur, Université Paris Cité, Image Analysis Hub,F-75015 Paris, France
Institutions: Pathogenèse des infections vasculaires; Université Paris Cité (France); University of California, Davis (United States)
Journal: EMBO reports, volume 27, issue 15, pages 4544-4582
Dates: received 15 October 2025; accepted 8 June 2026; published online 25 June 2026; in print August 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1038/s44319-026-00843-z · PMID 42350658 · PMCID PMC13458072 · OpenAlex W7165863193
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: human (organism), cellular / molecular (subfield)
Methods: Statistics, Preprocessing, Evoked potentials
Keywords: Cell Adhesion, Polarity & Cytoskeleton, Microbiology, Virology & Host Pathogen Interaction, Signal Transduction
MeSH: Actin Cytoskeleton*, Actins*, ADP-Ribosylation Factor 1*, Neisseria meningitidis*, Actin-Related Protein 2-3 Complex, cdc42 GTP-Binding Protein, Cell Membrane, Endothelial Cells, Humans, Wiskott-Aldrich Syndrome Protein, Neuronal (* major topic)
Topic: Bacterial Infections and Vaccines (Microbiology, Immunology and Microbiology), according to OpenAlex
Funding: Fondation pour la Recherche Médicale (Foundation for Medical Research in France) (EQU202203014610); Agence Nationale de la Recherche (French National Research Agency) (ANR 10-LBX-62 IBEID, ANR MeningoChip 18-CE15-0006-01, ANR-10-INBS-04, Investments for the Future, Investissements d'Avenir EQUIPEX CACSICE - ANR-11-EQPX-0008); European Research Council (ERC-AdG 101097791-DESTOP); Région île-de-France (DIM ELICIT2019, DIM1HEALTH); Université de Paris (University of Paris) (PhD fellowship from BioSPC doctoral school); Institut Pasteur (Pasteur Institute) (PFR-6-SI-COV2 project)
Citations: not cited yet (Europe PMC); 80 references in the paper
Research resources: IMOD (version 4.9.10) RRID:SCR_003297, SerialEM (version 3.7 to 4.1) RRID:SCR_017293
Notices: A correction to this paper has been published (42744926, from Europe PMC)

Abstract

Cells experience external forces that deform the plasma membrane to which they adapt by reorganizing their actin cytoskeleton. Here, using the extracellular bacterium Neisseria meningitidis as a model system, we explore how this bacterium reorganizes the cortical actin cytoskeleton subsequently to mechanical membrane deformations. Meningococci trigger the formation of tubular cellular plasma membrane protrusions by a previously described adhesion-driven process known as one-dimensional wetting. Cryo-electron tomography reveals that in epithelial cells such a deformation of the plasma membrane leads to the formation of F-actin bundles. In contrast, in endothelial cells a branched F-actin network is formed. By combining high resolution photonic microscopy approaches with genetic and drug perturbations in endothelial cells, we demonstrate that Arp2/3 activity is necessary for forming this branched network. We demonstrate the role of the nucleating-promoting factor N-WASP downstream of Cdc42. Proteomic analyses reveal the contribution of the small GTPase Arf1. Taken together, our results delineate an Arf1-Cdc42-N-WASP-Arp2/3 pathway that links mechanical plasma membrane deformation to the subsequent reorganization of a cortical branched F-actin network in endothelial cells.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

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Data

Data links

Data availability

The LC-MS/MS proteomic data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD045648 (http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD045648) and are available following this link: https://www.ebi.ac.uk/pride/archive/projects/PXD045648.

The source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44319-026-00843-z (https://www.ebi.ac.uk/biostudies/sourcedata/studies/S-SCDT-10_1038-S44319-026-00843-z).

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 19 authors, 3 keywords, 10 MeSH terms, 6 funders, 80 references, 2 RRIDs, 1 integrity notice.

Cite

This paper

Sahnine, M., Crochet, M., Tachon, S., Goussard, S., Pénard, E., Scandola, C., Duchateau, M., Giai Gianetto, Q., Salles, A., Moneron, G., Swistak, L., Charles-Orszag, A., Mallet, A., Matondo, M., Tinevez, J.-Y., Bonazzi, D., Sartori-Rupp, A., Duménil, G., & Obino, D. (2026). Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization. EMBO reports, 27(15), 4544-4582. https://doi.org/10.1038/s44319-026-00843-z

BibTeX

@article{sahnine2026arf1,
author = {Sahnine, Margot and Crochet, Morgane and Tachon, Stéphane and Goussard, Sylvie and Pénard, Esthel and Scandola, Cyril and Duchateau, Magalie and Giai Gianetto, Quentin and Salles, Audrey and Moneron, Gaël and Swistak, Léa and Charles-Orszag, Arthur and Mallet, Adeline and Matondo, Mariette and Tinevez, Jean-Yves and Bonazzi, Daria and Sartori-Rupp, Anna and Duménil, Guillaume and Obino, Dorian},
title = {{Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization}},
journal = {EMBO reports},
year = {2026},
month = jun,
volume = {27},
number = {15},
pages = {4544--4582},
publisher = {Nature Publishing Group},
issn = {1469-221X},
doi = {10.1038/s44319-026-00843-z},
url = {https://doi.org/10.1038/s44319-026-00843-z},
pmid = {42350658},
pmcid = {PMC13458072}
}

RIS

TY - JOUR
AU - Sahnine, Margot
AU - Crochet, Morgane
AU - Tachon, Stéphane
AU - Goussard, Sylvie
AU - Pénard, Esthel
AU - Scandola, Cyril
AU - Duchateau, Magalie
AU - Giai Gianetto, Quentin
AU - Salles, Audrey
AU - Moneron, Gaël
AU - Swistak, Léa
AU - Charles-Orszag, Arthur
AU - Mallet, Adeline
AU - Matondo, Mariette
AU - Tinevez, Jean-Yves
AU - Bonazzi, Daria
AU - Sartori-Rupp, Anna
AU - Duménil, Guillaume
AU - Obino, Dorian
TI - Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization
T2 - EMBO reports
J2 - EMBO Rep
PY - 2026
DA - 2026/06/25
VL - 27
IS - 15
SP - 4544
EP - 4582
SN - 1469-221X
PB - Nature Publishing Group
DO - 10.1038/s44319-026-00843-z
UR - https://doi.org/10.1038/s44319-026-00843-z
LA - en
ER -

CSL-JSON

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