Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1.
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Python · 1 line · 25 B · no license
- __all__ = ["ngs_helper"]
__init__.py at commit 5df2fb1, no license · at the source
Overview
- Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
- Nash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA
- Department of Orthopedics, Tongji Hospital, Tongji University School of Medicine, Shanghai, China
- Department of Orthopedics, Xi’an International Medical Center Hospital, Xi’an, China
- Department of Neurosurgery, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Abstract
Directional cues are essential for orienting cells during tissue morphogenesis and repair. In peripheral nerve regeneration, Schwann cells (SCs) align longitudinally in the nerve bridge to guide axonal pathfinding, but the mechanisms are not fully understood. We show here that after nerve injury, activated SCs up-regulate the guidance receptor Plexin-B1, enabling membrane plasticity required for SC polarization and longitudinal alignment along the axons. Aligned axon-SC provides positional cues to orient macrophages and extracellular matrix. Loss of Plexin-B1 disrupts SC morphological transformation, contact inhibition of locomotion between SCs, and axon-SC alignment, leading to SC misorientation, excessive inflammation, and delayed axon regeneration and functional recovery. These findings identify Plexin-B1 as a key orchestrator to orient SCs by regulating both SC-SC and axon-SC interactions during nerve repair. Elucidating the mechanisms of spatial guidance in nerve repair after injury has potential implications for therapeutic strategies to enhance neural regeneration.
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shenlab-sinai/NGS-Data-Charmer
5df2fb1a99b59e35ad415f07c06d6cc5a5ef26ab, 24 January 2024Availability: 1 check, the latest on 30 September 2026: the link answers
- 30 September 2026: the link answers
6 files
- ngs_helper/
__init__.py , Python, 1 line - ngs_helper/
generate_counts_matrix.p , Python, 21 linesy - ngs_helper/
ngs_helper.py , Python, 238 lines - run_snakemake.sh, Shell, 3 lines
- run_snakemake_cluster.sh
, Shell, 16 lines - README.md, Text, 151 lines
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Data
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 10 authors, 8 MeSH terms, 3 funders, 68 references.
Cite
This paper
Li, J., Ni, H., Halawani, D., Estill, M., Ramakrishnan, A., Alves, C. J., Shen, L., He, X., Friedel, R. H., & Zou, H. (2026). Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1. Science advances, 12(17), eadw4136. https://
BibTeX
@article{li2026direction
author = {Li, Jiaxi and Ni, Haofei and Halawani, Dalia and Estill, Molly and Ramakrishnan, Aarthi and Alves, Chrystian Junqueira and Shen, Li and He, Xijing and Friedel, Roland H and Zou, Hongyan},
title = {{Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1}},
journal = {Science advances},
year = {2026},
month = apr,
volume = {12},
number = {17},
pages = {eadw4136},
publisher = {American Association for the Advancement of Science},
issn = {2375-2548},
doi = {10.1126/
url = {https://
pmid = {42030397},
pmcid = {PMC13108569}
}
RIS
TY - JOUR
AU - Li, Jiaxi
AU - Ni, Haofei
AU - Halawani, Dalia
AU - Estill, Molly
AU - Ramakrishnan, Aarthi
AU - Alves, Chrystian Junqueira
AU - Shen, Li
AU - He, Xijing
AU - Friedel, Roland H
AU - Zou, Hongyan
TI - Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1
T2 - Science advances
J2 - Sci Adv
PY - 2026
DA - 2026/
VL - 12
IS - 17
SP - eadw4136
SN - 2375-2548
PB - American Association for the Advancement of Science
DO - 10.1126/
UR - https://
LA - en
ER -
CSL-JSON
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