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Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

Code ↔ Paper

3 matches between paragraphs of the paper and lines of its authors' code, computed by the harvester (lexical-v1). Click a colored paragraph or line to see its counterpart.

The 3 matches
  1. [1] § Materials and methods › M12- Simulation using cytosim ↔ src/sim/fibers/dynamic_fiber.h, lines 1–78 · score 0.61 · dynamic instability, minus ends, plus ends, MAPs, Cytosim, tubulin
  2. [2] § Materials and methods › M12- Simulation using cytosim ↔ src/sim/fibers/dynamic_fiber_prop.h, lines 12–130 · score 0.53 · force velocity relationship, stalled, shrinking, microtubules
  3. [3] § Materials and methods › M12- Simulation using cytosim ↔ src/sim/fibers/classic_fiber.h, lines 1–51 · score 0.52 · dynamic instability, classic, plus ends, Cytosim, tubulin, force

Paper

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The authors' code

C/C++ header · 169 lines · 4.7 KB · cecill-2.1 · 1 match

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Overview

Authors: Louis Cueff1, Loïc Schmitt1, Ewen Huet1, Sylvain Pastezeur1, Méline Coquil1, Talia Savary1, Anouk Sénard1, Jacques Pécréaux1, Hélène Bouvrais1
ORCID iDs: Louis Cueff, Loïc Schmitt, Ewen Huet, Sylvain Pastezeur, Talia Savary, Anouk Sénard, Jacques Pécréaux, Hélène Bouvrais
  1. CNRS, Univ Rennes, IGDR (Institut de Génétique et Développement de Rennes) – UMR 6290, Rennes, France
Institutions: Centre National de la Recherche Scientifique (France); Institut de génétique et de développement de Rennes (France); Université de Rennes (France)
Journal: PLoS genetics, volume 22, issue 6, article e1012196
Dates: received 21 October 2025; accepted 1 June 2026; published online 29 June 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1371/journal.pgen.1012196 · PMID 42371962 · PMCID PMC13340844 · OpenAlex W4404918954
Open access: gold, a free copy (OpenAlex)
Preprint: doi:10.1101/2024.11.29.624795
Status: code verified
Categories: C. elegans (organism), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics, Machine learning, Preprocessing, Evoked potentials, Connectivity, fMRI & imaging, Smoothing, state filtering, decompositions
MeSH: Caenorhabditis elegans*, Caenorhabditis elegans Proteins*, Microtubule-Associated Proteins*, Microtubules*, Spindle Apparatus*, Zygote*, Animals, Mitosis, Mutation, RNA Interference (* major topic)
Journal subjects: Biology and Life Sciences, Cell Biology, Cellular Structures and Organelles, Cytoskeleton, Microtubules, Developmental Biology, Embryology, Embryos, Centrosomes, Cell Processes, Cell Cycle and Cell Division, Anaphase, Genetics, Epigenetics, RNA interference, Gene expression, Genetic interference, Biochemistry, Nucleic acids, RNA, Research and Analysis Methods, Imaging Techniques, Fluorescence Imaging, Animal Studies, Experimental Organism Systems, Model Organisms, Caenorhabditis Elegans, Animal Models, Organisms, Eukaryota, Animals, Invertebrates, Nematoda, Caenorhabditis, Zoology, Cellular Types, Animal Cells, Germ Cells, OVA, Zygotes
Topic: Microtubule and mitosis dynamics (Cell Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Ligue Contre le Cancer (PhD thesis funding, 3 years, CARID grant, Finistère commitee, Ille et Vilaine & Deux-Sèvres committees, 2019, Côtes d’Armor & Ille-et-Vilaine committees, 2021); Région Bretagne (ARED 2025, PhD thesis funding); Agence Nationale de la Recherche (ANR-22-CE45-001601); Université de Rennes (Défis scientifiques, 2020)
Citations: cited by 1 paper (Europe PMC); 204 references in the paper

Abstract

In the Caenorhabditis elegans zygote, mutations in zyg-8DCLK1, the sole Doublecortin-family member, disrupt mitotic spindle positioning, as seen by immunofluorescence. Doublecortin proteins bind microtubules and are thought to stabilise or rigidify them. In the zygote, ZYG-8 only modestly affects microtubule growth and nucleation. We thus investigated whether these moderate dynamic perturbations alone could explain the spindle mispositioning observed in zyg-8 mutants. Using three complementary genetic perturbations—RNAi-mediated depletion of ZYG-8, its overexpression, and the thermosensitive zyg-8(or484ts) mutant (that disrupts microtubule binding)—we observed altered spindle pole oscillations and changes in microtubule cortical-contact behaviour, indicative of impaired cortical forces. Importantly, these phenotypes could not be fully explained by previously reported alterations in microtubule dynamics, suggesting an additional mechanism. Our findings indicate that ZYG-8 increases microtubule rigidity: ZYG-8 depletion or mutation led to more frequent microtubule bending and higher curvature and tortuosity. Simulations confirmed that reduced rigidity prolongs cortical contact lifetimes, an effect we experimentally observed in zyg-8(RNAi) embryos. Using custom biophysical assays, we showed that microtubule softening in zyg-8(RNAi) embryos and zyg-8 mutants reduced the efficiency of centring forces, leading to exaggerated spindle-pole oscillations. In mutants, the largest oscillations caused spindle poles to move closer to the cell periphery, preventing re-centring and resulting in spindle mispositioning and misorientation during late anaphase. Importantly, reducing cortical pulling forces rescued orientation defects, highlighting the importance of balanced pulling-pushing forces for proper spindle positioning. We propose that sufficient microtubule rigidity is essential for generating effective cortical pushing forces, potentially in synergy with other microtubule properties, which contribute to centring mechanisms that ensure accurate spindle orientation in late mitosis. Given that DCLK1 is frequently deregulated in human cancers and that accurate spindle positioning is essential for maintaining cell proliferation-differentiation balance, these findings may have implications for understanding how disruptions in microtubule mechanics contribute to carcinogenesis.

Reproduced under the paper's license (CC BY), from the paper cited above.

Repository

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Zenodo 14246919

License: cecill-2.1
State: the link answers, verified on 27 September 2026
Evidence: files inventoried
Size: 1 file
Software Heritage: not checked
Found in: the text, “M12- Simulation using cytosim”
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Tools: Matplotlib (2 files), NumPy (1 file)
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
  • 27 September 2026: the link answers (HTTP 200)
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At the source: doi.org/10.5281/zenodo.14246919

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 512 scripts, each with its path and the digest of its content;
  • 3 matches between paragraphs of the paper and lines of the code (method lexical-v1);
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Data Availability

All relevant data are within the manuscript and its Supporting Information files. Computational developments are shared on Zenodo, with the DOIs indicated in Methods.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 10 MeSH terms, 4 funders, 198 references.

Cite

This paper

Cueff, L., Schmitt, L., Huet, E., Pastezeur, S., Coquil, M., Savary, T., Sénard, A., Pécréaux, J., & Bouvrais, H. (2026). Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans. PLoS genetics, 22(6), e1012196. https://doi.org/10.1371/journal.pgen.1012196

BibTeX

@article{cueff2026microtubule,
author = {Cueff, Louis and Schmitt, Loïc and Huet, Ewen and Pastezeur, Sylvain and Coquil, Méline and Savary, Talia and Sénard, Anouk and Pécréaux, Jacques and Bouvrais, Hélène},
title = {{Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans}},
journal = {PLoS genetics},
year = {2026},
month = jun,
volume = {22},
number = {6},
pages = {e1012196},
publisher = {PLOS},
issn = {1553-7390},
doi = {10.1371/journal.pgen.1012196},
url = {https://doi.org/10.1371/journal.pgen.1012196},
pmid = {42371962},
pmcid = {PMC13340844}
}

RIS

TY - JOUR
AU - Cueff, Louis
AU - Schmitt, Loïc
AU - Huet, Ewen
AU - Pastezeur, Sylvain
AU - Coquil, Méline
AU - Savary, Talia
AU - Sénard, Anouk
AU - Pécréaux, Jacques
AU - Bouvrais, Hélène
TI - Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans
T2 - PLoS genetics
J2 - PLoS Genet
PY - 2026
DA - 2026/06/29
VL - 22
IS - 6
SP - e1012196
SN - 1553-7390
PB - PLOS
DO - 10.1371/journal.pgen.1012196
UR - https://doi.org/10.1371/journal.pgen.1012196
LA - en
ER -

CSL-JSON

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