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Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model.

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Paper

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The authors' code

Python · 1 line · 0 B · no license

__init__.py at commit 7624b49, no license · at the source

Overview

Authors: Kazuaki Okamura1, Karoline Pichlerova1,2, Takayuki Matsuo1, Daisuke Watanabe3,4, William D. Cutts5, Brandon J. Kim5, Yoichi Morofuji1,6
ORCID iDs: Kazuaki Okamura, Karoline Pichlerova, William D. Cutts, Yoichi Morofuji
  1. Department of Neurosurgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  2. Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia
  3. Department of Medical Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
  4. BBB Laboratory, PharmaCo-Cell Co., Ltd., Nagasaki City, Japan
  5. Department of Biological Sciences, University of Texas at Dallas, Richardson, Texas, United States of America
  6. Department of Neurosurgery, Showa Medical University School of Medicine, Tokyo, Japan
Institutions: Nagasaki University (Japan); Slovak Academy of Sciences (Slovakia); Institute of Neuroimmunology of the Slovak Academy of Sciences (Slovakia); Pharmaco-Cell Company Ltd. (Japan) (Japan); The University of Texas at Dallas (United States); SHOWA Medical University (Japan)
Journal: PloS one, volume 21, issue 6, article e0352334
Dates: received 18 March 2026; accepted 9 June 2026; published online 29 June 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1371/journal.pone.0352334 · PMID 42371908 · PMCID PMC13313372 · OpenAlex W7166505076
Open access: gold, a free copy (OpenAlex)
Status: code verified
Categories: cellular / molecular (subfield)
Methods: Statistics
MeSH: Blood-Brain Barrier*, Dexamethasone*, Heat Stroke*, Animals, Endothelial Cells, Pericytes, Zonula Occludens-1 Protein (* major topic)
Journal subjects: Biology and Life Sciences, Anatomy, Nervous System, Central Nervous System, Blood-Brain Barrier, Medicine and Health Sciences, Physical Sciences, Materials Science, Material Properties, Permeability, Cell Biology, Cellular Types, Animal Cells, Pericytes, Epithelial Cells, Endothelial Cells, Biological Tissue, Epithelium, Cell Physiology, Junctional Complexes, Tight Junctions, Clinical Medicine, Signs and Symptoms, Hyperthermia, Engineering and Technology, Industrial Engineering, Process Engineering, Industrial Processes, Manufacturing Processes, Heat Treatment, Glial Cells, Macroglial Cells, Astrocytes
Topic: Thermoregulation and physiological responses (Physiology, Medicine), according to OpenAlex
Funding: Japan Society for the Promotion of Science (JPJSBP120203804, JPJSBP120219945, 20KK0254, 20K09351)
Citations: not cited yet (Europe PMC); 33 references in the paper
Research resources: The JAnaP RRID:SCR_026027

Abstract

Heat-related diseases and their treatments are becoming the center of focus due to global warming resulting in rising global temperatures. Heatstroke is the most hazardous condition of heat-related diseases, which when left untreated leads to death. One of the main characteristics of heatstroke is the dysfunction of the central nervous system. In this study, we established an in vitro heatstroke model of the blood–brain barrier (BBB) consisting of endothelial cells and pericytes. Following heat exposure at 43°C for 3 h, the model failed to recover during the subsequent 24 h regeneration period. The damage was shown by decreased transendothelial resistance (p < 0,0001) and confirmed by permeability assays and immunohistochemistry with in silico analysis. We subsequently evaluated the effect of dexamethasone in our heatstroke model. Administration of dexamethasone post-heatstroke alleviated BBB damage during the regeneration period, by increasing transendothelial electrical resistance and ZO-1 expression while reducing BBB permeability. Our findings suggest that dexamethasone reduces heatstroke damage at the BBB in in vitro conditions.

Reproduced under the paper's license (CC BY), from the paper cited above.

Repository

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StrokaLab/JAnaP

License: none: the authors keep all their rights
State: the link answers, verified on 27 September 2026
Evidence: files inventoried
Commit: 7624b4931fc1e545560e95d18b15017c244dab92, 24 June 2021
Languages: Python (53), JavaScript (3), Jupyter (1)
Size: 102 files, 57 scripts
Software Heritage: not archived
Found in: the text, “In silico analysis of ZO-1 tight junction marker”
Holds: README, environment (bin/requirements.txt), documentation, 1 notebook
Not found: license file, CITATION.cff, tests, continuous integration
Tools: NumPy (13 files), OpenCV (11 files), scikit-image (11 files), Matplotlib (8 files), SciPy (8 files), Pillow (1 file)
Availability: 1 check, the latest on 27 September 2026: the link answers
  • 27 September 2026: the link answers
58 files
At the source: github.com/StrokaLab/JAnaP

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

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  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 57 scripts, each with its path and the digest of its content;
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Data

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Data Availability

All relevant data are within the paper.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 7 MeSH terms, 1 funder, 32 references, 1 RRID.

Cite

This paper

Okamura, K., Pichlerova, K., Matsuo, T., Watanabe, D., Cutts, W. D., Kim, B. J., & Morofuji, Y. (2026). Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model. PloS one, 21(6), e0352334. https://doi.org/10.1371/journal.pone.0352334

BibTeX

@article{okamura2026dexamethasone,
author = {Okamura, Kazuaki and Pichlerova, Karoline and Matsuo, Takayuki and Watanabe, Daisuke and Cutts, William D. and Kim, Brandon J. and Morofuji, Yoichi},
title = {{Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model}},
journal = {PloS one},
year = {2026},
month = jun,
volume = {21},
number = {6},
pages = {e0352334},
publisher = {PLOS},
issn = {1932-6203},
doi = {10.1371/journal.pone.0352334},
url = {https://doi.org/10.1371/journal.pone.0352334},
pmid = {42371908},
pmcid = {PMC13313372}
}

RIS

TY - JOUR
AU - Okamura, Kazuaki
AU - Pichlerova, Karoline
AU - Matsuo, Takayuki
AU - Watanabe, Daisuke
AU - Cutts, William D.
AU - Kim, Brandon J.
AU - Morofuji, Yoichi
TI - Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model
T2 - PloS one
J2 - PLoS One
PY - 2026
DA - 2026/06/29
VL - 21
IS - 6
SP - e0352334
SN - 1932-6203
PB - PLOS
DO - 10.1371/journal.pone.0352334
UR - https://doi.org/10.1371/journal.pone.0352334
LA - en
ER -

CSL-JSON

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