OSCR

Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.

Overview

Authors: Yan Jiang1, Yuhu Feng1, Xinyu Li1, Bing Zhao2, Zuisu Yang1, Ruling Wei3, Falei Yuan1
ORCID iDs: Falei Yuan
  1. Department of Pharmacy, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China
  2. Department of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
  3. Department of Physical Education, Zhejiang Ocean University, Zhoushan, China
Journal: PloS one, volume 21, issue 8, article e0356781
Dates: received 6 February 2026; accepted 7 August 2026; published online 26 August 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1371/journal.pone.0356781 · PMID 42647552 · PMCID PMC13513980 · OpenAlex W7204247593
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: histology / microscopy (modality), mouse (organism), other (organism), cellular / molecular (subfield)
Methods: Statistics
MeSH: Brain*, Lipopolysaccharides*, Serum Albumin, Bovine*, Animals, Antigens, CD, Blood-Brain Barrier, Capillary Permeability, Cattle, CD68 Molecule, Male, Mice (* major topic)
Journal subjects: Biology and Life Sciences, Biochemistry, Proteins, Albumins, Medicine and Health Sciences, Clinical Medicine, Signs and Symptoms, Sepsis, Lipids, Research and Analysis Methods, Specimen Preparation and Treatment, Staining, Immunostaining, Precipitation Techniques, Immunoprecipitation, Vascular Medicine, Vascular Permeability, Enzymology, Enzymes, Peroxidases, Immunofluorescence Staining
Topic: Protein Interaction Studies and Fluorescence Analysis (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: National Natural Science Foundation of China (National Science Foundation of China) (82101382); Zhejiang Education '14th Five-Year Plan' Second Batch of Teaching Reform Project: Research on the Integration of Sports and Medicine in Medical Vocational Colleges Empowered by Digital Intelligence (jg20240009)
Citations: not cited yet (Europe PMC); 56 references in the paper

Abstract

This study examined the role of bovine serum albumin (BSA) in lipopolysaccharide (LPS)-induced brain injury. Mice were intraperitoneally injected with either LPS or LPS coupled with BSA. Brain tissues were analyzed for autofluorescence, vascular cell permeability, blood-brain barrier integrity, and pyroptosis. Co-immunoprecipitation and immunostaining were performed to assess the interaction between albumin and LPS. The results showed that, compared to the 5 mg/kg LPS group, the 5 mg/kg BSA-LPS group exhibited enhanced autofluorescence and an increased number of cells with co-localization of propidium iodide (PI) with cluster of differentiation 31 (CD31) and CD13. However, no significant differences were observed between the 25 mg/kg LPS and 25 mg/kg BSA-LPS groups. Horseradish peroxidase (HRP) labeling revealed higher HRP leakage in the 5 mg/kg BSA-LPS group compared to the 5 mg/kg LPS group, a difference that disappeared at the 25 mg/kg dose. Peroxidase staining showed a similar trend. Immunostaining for gasdermin D and CD11b showed significant differences only between the 5 mg/kg LPS and 5 mg/kg BSA-LPS groups. CD68 immunostaining exhibited a similar trend. Furthermore, the study confirmed that albumin binds to LPS in the brain, with more abundant Lipid A+ signals detected in the 5 mg/kg BSA-LPS group compared to the 5 mg/kg LPS group. These findings collectively suggest that albumin boosts the effect of LPS in the brain, probably by binding with LPS and increasing its entry into the brain parenchyma.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data

Datasets cited

Data Availability

The raw data is stored in the Figshare, and the link is https://doi.org/10.6084/m9.figshare.32668677.v2, https://doi.org/10.6084/m9.figshare.32669166.v2, https://doi.org/10.6084/m9.figshare.32669916 and https://doi.org/10.6084/m9.figshare.32669013.v2.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 7 authors, 11 MeSH terms, 2 funders, 56 references.

Cite

This paper

Jiang, Y., Feng, Y., Li, X., Zhao, B., Yang, Z., Wei, R., & Yuan, F. (2026). Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides. PloS one, 21(8), e0356781. https://doi.org/10.1371/journal.pone.0356781

BibTeX

@article{jiang2026bovine,
author = {Jiang, Yan and Feng, Yuhu and Li, Xinyu and Zhao, Bing and Yang, Zuisu and Wei, Ruling and Yuan, Falei},
title = {{Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides}},
journal = {PloS one},
year = {2026},
month = aug,
volume = {21},
number = {8},
pages = {e0356781},
publisher = {PLOS},
issn = {1932-6203},
doi = {10.1371/journal.pone.0356781},
url = {https://doi.org/10.1371/journal.pone.0356781},
pmid = {42647552},
pmcid = {PMC13513980}
}

RIS

TY - JOUR
AU - Jiang, Yan
AU - Feng, Yuhu
AU - Li, Xinyu
AU - Zhao, Bing
AU - Yang, Zuisu
AU - Wei, Ruling
AU - Yuan, Falei
TI - Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides
T2 - PloS one
J2 - PLoS One
PY - 2026
DA - 2026/08/26
VL - 21
IS - 8
SP - e0356781
SN - 1932-6203
PB - PLOS
DO - 10.1371/journal.pone.0356781
UR - https://doi.org/10.1371/journal.pone.0356781
LA - en
ER -

CSL-JSON

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