Altered brain vascularization and transcriptional changes in embryos lacking ABCA1 support a role of cholesterol in brain angiogenesis.
Overview
- Instituto de Ciencias de la Salud, Universidad de O´Higgins, Rancagua, Chile
- Centro de Investigación e Innovación Biomédica, Universidad de los Andes, Santiago, Chile
- Laboratory of Stem Cells and Developmental Biology, Faculty of Sciences, Universidad de Chile, Santiago, Chile
- Department of Pediatrics, University of California, San Francisco, CA, United States
- Newborn Brain Research Institute, University of California, San Francisco, CA, United States
Abstract
Introduction: Lipoproteins are the main lipid carriers in extracellular fluids and mediate the exchange of hydrophobic molecules between cells and their environment in animals, including cholesterol. Lipoprotein metabolism has been implicated in the regulation of angiogenesis, suggesting that cholesterol is important for this process, but a direct role for this lipid has not been unequivocally demonstrated, particularly in the central nervous system (CNS).
Methods: Here, we used genetic and pharmacological models to address this issue in different models of CNS angiogenesis.
Results: We show that inactivation of ABCA1, one of the main cholesterol efflux pumps, leads to altered brain vascularization and a longer angiogenic window, while inactivation of the bidirectional cholesterol transporter SR-B1 has no effect. These functional changes are accompanied by consistent transcriptomic changes in genes involved in cholesterol synthesis and angiogenesis. In support of the role of cholesterol, experimental reduction of this lipid in cultured brain endothelial cells leads to a transcriptomic signature showing the opposite direction in those genes.
Discussion: These studies support a role for ABCA1 and cholesterol in regulating a trascriptional program governing angiogenesis in the brain.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- geo:GSE73721, at NCBI GEO; found in the text, “RNA sequencing”
Data availability statement
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Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 29 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 13 authors, 5 keywords, 1 funder, 35 references.
Cite
This paper
Becerra, B., Ramírez-Herrera, O., Barrios, J., Madrid, J., Calfunao, S., Saavedra, F., Romo-Toledo, P., Juárez-Balarezo, J., Casanova-Maldonado, I., Arnold, T., Palma, V., Busso, D., & Santander, N. (2026). Altered brain vascularization and transcriptional changes in embryos lacking ABCA1 support a role of cholesterol in brain angiogenesis. Frontiers in cell and developmental biology, 14, 1783696. https://
BibTeX
@article{becerra2026alte
author = {Becerra, Brenda and Ramírez-Herrera, Oriana and Barrios, Javiera and Madrid, Josefina and Calfunao, Susan and Saavedra, Fujiko and Romo-Toledo, Patricia and Juárez-Balarezo, Jesús and Casanova-Maldonado, Ignacio and Arnold, Thomas and Palma, Verónica and Busso, Dolores and Santander, Nicolas},
title = {{Altered brain vascularization and transcriptional changes in embryos lacking ABCA1 support a role of cholesterol in brain angiogenesis}},
journal = {Frontiers in cell and developmental biology},
year = {2026},
month = apr,
volume = {14},
pages = {1783696},
publisher = {Frontiers Media SA},
issn = {2296-634X},
doi = {10.3389/
url = {https://
pmid = {42099390},
pmcid = {PMC13143920}
}
RIS
TY - JOUR
AU - Becerra, Brenda
AU - Ramírez-Herrera, Oriana
AU - Barrios, Javiera
AU - Madrid, Josefina
AU - Calfunao, Susan
AU - Saavedra, Fujiko
AU - Romo-Toledo, Patricia
AU - Juárez-Balarezo, Jesús
AU - Casanova-Maldonado, Ignacio
AU - Arnold, Thomas
AU - Palma, Verónica
AU - Busso, Dolores
AU - Santander, Nicolas
TI - Altered brain vascularization and transcriptional changes in embryos lacking ABCA1 support a role of cholesterol in brain angiogenesis
T2 - Frontiers in cell and developmental biology
J2 - Front Cell Dev Biol
PY - 2026
DA - 2026/
VL - 14
SP - 1783696
SN - 2296-634X
PB - Frontiers Media SA
DO - 10.3389/
UR - https://
LA - en
ER -
CSL-JSON
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