OSCR

Zebrafish study provides evidence for <i>Porphyromonas gingivalis</i> outer membrane vesicles eliciting Alzheimer's disease-like pathologies.

Overview

Authors: Jianbin Guo1, Kaijin Lin1,2, Ruiyun Liang1,2, Lingxia Jiang3, Xiaozhen He3, Jiawei Chen1,2, Minqian Zheng1,2
ORCID iDs: Xiaozhen He
  1. Clinical Research Center for Oral Tissue Deficiency Diseases of Fujian Province & Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China
  2. Stomatological Key laboratory of Fujian College and University & Institute of Stomatology & Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China
  3. Institute of Life Sciences, College of Biological Science and Engineering, Fuzhou University, Fuzhou, China
Institutions: Fujian Medical University (China); Fuzhou University (China)
Journal: Frontiers in cellular and infection microbiology, volume 16, article 1761068
Dates: received 5 December 2025; accepted 18 February 2026; published online 6 March 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.3389/fcimb.2026.1761068 · PMID 41868145 · PMCID PMC13002584 · OpenAlex W7134031534
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), zebrafish (organism), Alzheimer's / dementia (population), cellular / molecular (subfield)
Methods: Statistics
Keywords: periodontitis, alzheimer’s disease, porphyromonas gingivalis, outer membrane vesicle, Zebrafish
MeSH: Alzheimer Disease*, Bacterial Outer Membrane*, Porphyromonas gingivalis*, Acetylcholinesterase, Amyloid beta-Peptides, Animals, Brain, Disease Models, Animal, Gene Expression Profiling, Larva, Peptide Fragments, Periodontitis, Proteomics, Zebrafish (* major topic)
Topic: Oral microbiology and periodontitis research (Periodontics, Dentistry), according to OpenAlex
Funding: Fujian Provincial Health Technology Project ((2021GGB016) (2022GGA041) (2023QNA068)（2021GGB016）（2022GGA041）（2023QNA068）)
Citations: not cited yet (Europe PMC); 42 references in the paper

Abstract

Introduction: Periodontitis has been epidemiologically linked to an increased risk of Alzheimer’s disease (AD), yet the mechanistic contribution of periodontal pathogens remains insufficiently understood. Building on our previous findings that Porphyromonas gingivalis outer membrane vesicles (OMVs) induce cardiovascular dysfunction, this study investigates whether these vesicles also drive AD‐related pathology using the zebrafish model.

Methods: We microinjected P. gingivalis OMVs into the common cardinal vein of zebrafish larvae to evaluate locomotor behavior, brain injury, and neuroinflammatory responses. Integrated proteomic and transcriptomic analyses were performed to identify alterations in AD‐associated pathways, and acetylcholinesterase activity along with Aβ1–42 plaque accumulation were quantified to validate hallmark AD phenotypes.

Results: OMV exposure resulted in significant neurotoxicity, locomotor deficits, and robust neuroinflammation, accompanied by pronounced dysregulation of AD-related molecular pathways. Notably, OMVs markedly increased acetylcholinesterase activity and promoted Aβ1–42 deposition in larval brains.

Discussion: These findings demonstrate that P. gingivalis OMVs act as potent inducers of neuronal damage and AD-like pathological features in vivo, providing mechanistic insight into how periodontal pathogens may contribute to neurodegenerative disease progression.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data, not to its authors' code: see the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none, so it has no map.

Data

Datasets cited

Data availability statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 7 authors, 5 keywords, 14 MeSH terms, 1 funder, 42 references.

Cite

This paper

Guo, J., Lin, K., Liang, R., Jiang, L., He, X., Chen, J., & Zheng, M. (2026). Zebrafish study provides evidence for <i>Porphyromonas gingivalis</i> outer membrane vesicles eliciting Alzheimer's disease-like pathologies. Frontiers in cellular and infection microbiology, 16, 1761068. https://doi.org/10.3389/fcimb.2026.1761068

BibTeX

@article{guo2026zebrafish,
author = {Guo, Jianbin and Lin, Kaijin and Liang, Ruiyun and Jiang, Lingxia and He, Xiaozhen and Chen, Jiawei and Zheng, Minqian},
title = {{Zebrafish study provides evidence for \<i\>Porphyromonas gingivalis\</i\> outer membrane vesicles eliciting Alzheimer's disease-like pathologies}},
journal = {Frontiers in cellular and infection microbiology},
year = {2026},
month = mar,
volume = {16},
pages = {1761068},
publisher = {Frontiers Media SA},
issn = {2235-2988},
doi = {10.3389/fcimb.2026.1761068},
url = {https://doi.org/10.3389/fcimb.2026.1761068},
pmid = {41868145},
pmcid = {PMC13002584}
}

RIS

TY - JOUR
AU - Guo, Jianbin
AU - Lin, Kaijin
AU - Liang, Ruiyun
AU - Jiang, Lingxia
AU - He, Xiaozhen
AU - Chen, Jiawei
AU - Zheng, Minqian
TI - Zebrafish study provides evidence for <i>Porphyromonas gingivalis</i> outer membrane vesicles eliciting Alzheimer's disease-like pathologies
T2 - Frontiers in cellular and infection microbiology
J2 - Front Cell Infect Microbiol
PY - 2026
DA - 2026/03/06
VL - 16
SP - 1761068
SN - 2235-2988
PB - Frontiers Media SA
DO - 10.3389/fcimb.2026.1761068
UR - https://doi.org/10.3389/fcimb.2026.1761068
LA - en
ER -

CSL-JSON

{
"id": "10.3389/fcimb.2026.1761068",
"type": "article-journal",
"title": "Zebrafish study provides evidence for <i>Porphyromonas gingivalis</i> outer membrane vesicles eliciting Alzheimer's disease-like pathologies",
"container-title": "Frontiers in cellular and infection microbiology",
"author": [
{
"family": "Guo",
"given": "Jianbin"
},
{
"family": "Lin",
"given": "Kaijin"
},
{
"family": "Liang",
"given": "Ruiyun"
},
{
"family": "Jiang",
"given": "Lingxia"
},
{
"family": "He",
"given": "Xiaozhen"
},
{
"family": "Chen",
"given": "Jiawei"
},
{
"family": "Zheng",
"given": "Minqian"
}
],
"container-title-short": "Front Cell Infect Microbiol",
"volume": "16",
"page": "1761068",
"DOI": "10.3389/fcimb.2026.1761068",
"PMID": "41868145",
"PMCID": "PMC13002584",
"ISSN": "2235-2988",
"publisher": "Frontiers Media SA",
"URL": "https://doi.org/10.3389/fcimb.2026.1761068",
"language": "en",
"issued": {
"date-parts": [
[
2026,
3,
6
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.3389/fnagi.2026.1761218
Integrated transcriptomic profiling combined with <i>in vitro</i> validation reveals the involvement of TMEM140 in the link between periodontitis and brain aging.
Journal: Frontiers in aging neuroscience
In common: Alzheimer's / dementia, genetics / omics, cellular / molecular, 4 references
[2] doi:10.1002/alz.71433
Genetic correlation analysis of Alzheimer's disease and stroke implicates PHLPP1 as a shared locus in individuals of African ancestry.
Journal: Alzheimer's & dementia : the journal of the Alzheimer's Association
In common: Alzheimer's / dementia, genetics / omics, cellular / molecular, 2 references
[3] doi:10.3390/ijms27083430
Metatranscriptomic Reanalysis of Alzheimer's Brains Identifies Low-Biomass Microbial Signals Including Enrichment of <i>Acinetobacter radioresistens</i>.
Journal: International journal of molecular sciences
In common: Alzheimer's / dementia, genetics / omics, 2 references
[4] doi:10.3390/ijms27177542
Assessment of the Association of Periodontitis and Diabetes Mellitus with Alzheimer's Disease in a Mouse Model.
Journal: International journal of molecular sciences
In common: Alzheimer's / dementia, cellular / molecular, 2 references
[5] doi:10.1016/j.isci.2026.116336 [code]
Transcriptomic signatures of synaptic loss in Alzheimer's disease.
Journal: iScience
In common: Alzheimer's / dementia, genetics / omics, cellular / molecular, 2 references
[6] doi:10.1038/s43587-026-01204-0
Fibronectin mediates APOE4-driven blood-brain barrier dysfunction in Alzheimer's disease.
Journal: Nature aging
In common: Alzheimer's / dementia, genetics / omics, cellular / molecular, 1 reference
[7] doi:10.1002/alz.71471 [code]
How does type 2 diabetes modify the risk of Alzheimer's disease?
Journal: Alzheimer's & dementia : the journal of the Alzheimer's Association
In common: Alzheimer's / dementia, genetics / omics, cellular / molecular, 1 reference
[8] doi:10.1371/journal.pcbi.1014074 [code]
Interpretable integration of unpaired multi-omics for Alzheimer's diagnosis via cross-modal transformer reconstruction.
Journal: PLoS computational biology
In common: Alzheimer's / dementia, genetics / omics, 1 reference
[9] doi:10.1016/j.xpro.2026.104766 [code]
Protocol for live-imaging of blood flow and vascular permeability in zebrafish larvae.
Journal: STAR protocols
In common: zebrafish, 1 reference
[10] doi:10.1186/s12974-026-03800-8
CMV titer associations with cognition and the plasma proteome implicate FLT1 and neurovascular mechanisms as potential moderators.
Journal: Journal of neuroinflammation
In common: Alzheimer's / dementia, genetics / omics, 1 reference

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.

Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.