OSCR

<i>Roseburia</i>-Associated Gut-Brain Axis Alterations in Relapsing-Remitting Multiple Sclerosis: Evidence from a Household-Matched Case-Control Study.

Overview

Authors: Alen Zollo1,2, Matteo Domenico Marsiglia1, Andrea Corona1,2, Emerenziana Ottaviano1, Maria Laura Terzi Mazzieri1, Alessandra Mingione1,2, Silvia Ancona1, Alberto Priori1,2,3, Elisa Borghi1, Filippo Martinelli-Boneschi1,2,3
ORCID iDs: Alen Zollo, Matteo Domenico Marsiglia, Andrea Corona, Emerenziana Ottaviano, Alessandra Mingione, Silvia Ancona, Alberto Priori, Elisa Borghi, Filippo Martinelli-Boneschi
  1. Department of Health Sciences, University of Milan, 20142 Milan, Italy; (A.Z.); (M.D.M.); (A.C.); (E.O.); (M.L.T.M.); (A.M.); (S.A.); (A.P.); (M.-B.F.)
  2. CRC “Aldo Ravelli” for Experimental Brain Therapeutics, University of Milan, 20142 Milan, Italy
  3. Clinical Neurology Unit, San Paolo Hospital, ASST Santi Paolo e Carlo, 20142 Milan, Italy
Institutions: University of Milan (Italy); Ospedale San Paolo (Italy); Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo (Italy)
Journal: Nutrients, volume 18, issue 7, article 1117
Dates: received 4 February 2026; accepted 26 March 2026; published online 31 March 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.3390/nu18071117 · PMID 41978167 · PMCID PMC13074294 · OpenAlex W7144047456
Open access: gold, a free copy (OpenAlex)
Status: data only (how the code is found and verified)
Categories: human (organism), multiple sclerosis (population), clinical / translational (subfield)
Methods: Statistics, Machine learning
Keywords: multiple sclerosis, microbiota–gut–brain axis, Roseburia, clinical severity
MeSH: Brain-Gut Axis*, Dysbiosis*, Eubacteriales*, Gastrointestinal Microbiome*, Multiple Sclerosis, Relapsing-Remitting*, Adult, Case-Control Studies, Fatty Acids, Volatile, Feces, Female, Humans, Male, Middle Aged, RNA, Ribosomal, 16S, Severity of Illness Index (* major topic)
Topic: Gut microbiota and health (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Italian Ministry of Research (# PNC0000001)
Citations: not cited yet (Europe PMC); 34 references in the paper

Abstract

Background/Objectives: Gut microbiota (GM) dysbiosis has been implicated in multiple sclerosis (MS) pathogenesis, influencing inflammation and neurodegeneration, but findings remain inconsistent due to environmental and methodological variability. This study aimed to identify possible microbial biomarkers of MS status and disease severity by profiling gut microbiota and short-chain fatty acid (SCFA) patterns in people with relapsing–remitting MS (pwRRMS), using household-matched healthy controls (HC) to minimize environmental variability. Methods: Twenty-four pwRRMS and their respective household-matched healthy controls (HC) were enrolled, with dietary and lifestyle habits monitored. GM composition was assessed by 16S rRNA gene sequencing, and fecal SCFAs were quantified using gas chromatography–mass spectrometry. PwRRMS were stratified by Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results: β-diversity did not differ between groups. However, α-diversity was significantly reduced in pwRRMS, particularly in those with greater disability. Reduced diversity was associated with lower abundance of butyrate-producing genera (Roseburia, Faecalibacterium, Coprococcus) and enrichment of Oscillibacter and UBA1819, alongside a downward trend in fecal butyrate and propionate levels. Conclusions: RRMS and greater disease severity are associated with gut microbial alterations characterized by reduced SCFA-producing bacteria. Despite limitations including small sample size and sex imbalance, the household-matched design strengthens internal validity. Our findings highlight the potential of targeting the gut microbiota, an accessible compartment within the gut–brain axis, for MS management.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper links to its data and to no code of its authors: see the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none, so it has no map.

Data

Data links

Data Availability Statement

The data presented in this study are openly available in the NCBI Short Read Archive (SRA, http://www.ncbi.nlm.nih.gov/sra, accessed on 27 March 2026) under accession number PRJNA1295419.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 10 authors, 4 keywords, 15 MeSH terms, 1 funder, 33 references.

Cite

This paper

Zollo, A., Marsiglia, M. D., Corona, A., Ottaviano, E., Mazzieri, M. L. T., Mingione, A., Ancona, S., Priori, A., Borghi, E., & Martinelli-Boneschi, F. (2026). <i>Roseburia</i>-Associated Gut-Brain Axis Alterations in Relapsing-Remitting Multiple Sclerosis: Evidence from a Household-Matched Case-Control Study. Nutrients, 18(7), 1117. https://doi.org/10.3390/nu18071117

BibTeX

@article{zollo2026lt,
author = {Zollo, Alen and Marsiglia, Matteo Domenico and Corona, Andrea and Ottaviano, Emerenziana and Mazzieri, Maria Laura Terzi and Mingione, Alessandra and Ancona, Silvia and Priori, Alberto and Borghi, Elisa and Martinelli-Boneschi, Filippo},
title = {{\<i\>Roseburia\</i\>-Associated Gut-Brain Axis Alterations in Relapsing-Remitting Multiple Sclerosis: Evidence from a Household-Matched Case-Control Study}},
journal = {Nutrients},
year = {2026},
month = mar,
volume = {18},
number = {7},
pages = {1117},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
issn = {2072-6643},
doi = {10.3390/nu18071117},
url = {https://doi.org/10.3390/nu18071117},
pmid = {41978167},
pmcid = {PMC13074294}
}

RIS

TY - JOUR
AU - Zollo, Alen
AU - Marsiglia, Matteo Domenico
AU - Corona, Andrea
AU - Ottaviano, Emerenziana
AU - Mazzieri, Maria Laura Terzi
AU - Mingione, Alessandra
AU - Ancona, Silvia
AU - Priori, Alberto
AU - Borghi, Elisa
AU - Martinelli-Boneschi, Filippo
TI - <i>Roseburia</i>-Associated Gut-Brain Axis Alterations in Relapsing-Remitting Multiple Sclerosis: Evidence from a Household-Matched Case-Control Study
T2 - Nutrients
J2 - Nutrients
PY - 2026
DA - 2026/03/31
VL - 18
IS - 7
SP - 1117
SN - 2072-6643
PB - Multidisciplinary Digital Publishing Institute (MDPI)
DO - 10.3390/nu18071117
UR - https://doi.org/10.3390/nu18071117
LA - en
ER -

CSL-JSON

{
"id": "10.3390/nu18071117",
"type": "article-journal",
"title": "<i>Roseburia</i>-Associated Gut-Brain Axis Alterations in Relapsing-Remitting Multiple Sclerosis: Evidence from a Household-Matched Case-Control Study",
"container-title": "Nutrients",
"author": [
{
"family": "Zollo",
"given": "Alen"
},
{
"family": "Marsiglia",
"given": "Matteo Domenico"
},
{
"family": "Corona",
"given": "Andrea"
},
{
"family": "Ottaviano",
"given": "Emerenziana"
},
{
"family": "Mazzieri",
"given": "Maria Laura Terzi"
},
{
"family": "Mingione",
"given": "Alessandra"
},
{
"family": "Ancona",
"given": "Silvia"
},
{
"family": "Priori",
"given": "Alberto"
},
{
"family": "Borghi",
"given": "Elisa"
},
{
"family": "Martinelli-Boneschi",
"given": "Filippo"
}
],
"container-title-short": "Nutrients",
"volume": "18",
"issue": "7",
"page": "1117",
"DOI": "10.3390/nu18071117",
"PMID": "41978167",
"PMCID": "PMC13074294",
"ISSN": "2072-6643",
"publisher": "Multidisciplinary Digital Publishing Institute (MDPI)",
"URL": "https://doi.org/10.3390/nu18071117",
"language": "en",
"issued": {
"date-parts": [
[
2026,
3,
31
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.1128/msystems.00416-26 [code]
Integrative multicohort analysis reveals consistent sex differences in gut microbiota of multiple sclerosis patients.
Journal: mSystems
In common: multiple sclerosis, clinical / translational, 4 references
[2] doi:10.3389/fnins.2025.1655456
Gut microbiota signatures in tuberous sclerosis complex and epilepsy: a pilot study.
Journal: Frontiers in neuroscience
In common: 1 reference, author Matteo Domenico Marsiglia
[3] doi:10.1038/s41531-022-00399-4
Amplitude and frequency modulation of subthalamic beta oscillations jointly encode the dopaminergic state in Parkinson's disease.
Journal: NPJ Parkinson's disease
In common: author Alberto Priori
[4] doi:10.64898/2026.03.16.711194
Female-enriched <i>Eggerthella lenta</i> drives neuroinflammation and IFN-γ via host receptor TLR2
Journal: bioRxiv (preprint)
In common: multiple sclerosis, 2 references
[5] doi:10.1038/s41598-024-56989-0
Gut microbiota profile in CDKL5 deficiency disorder patients.
Journal: Scientific reports
In common: clinical / translational, 2 references
[6] doi:10.1038/s41467-025-56829-3 [code]
Machine learning-based meta-analysis reveals gut microbiome alterations associated with Parkinson's disease.
Journal: Nature communications
In common: clinical / translational, 2 references
[7] doi:10.1038/s41467-023-38248-4 [code]
Gut microbiome dysbiosis across early Parkinson's disease, REM sleep behavior disorder and their first-degree relatives.
Journal: Nature communications
In common: clinical / translational, 2 references
[8] doi:10.1128/spectrum.00609-26
Integrated multi-omics analysis reveals gut dysbiosis and altered energy metabolism in Chinese ALS patients.
Journal: Microbiology spectrum
In common: 2 references
[9] doi:10.1186/s40168-025-02095-w [code]
Distinct gut microbiome characteristics and dynamics in patients with Parkinson's disease based on the presence of premotor rapid-eye movement sleep behavior disorders.
Journal: Microbiome
In common: clinical / translational, 2 references
[10] doi:10.1136/gpsych-2023-101374
Gut microbiota-derived short-chain fatty acids and depression: deep insight into biological mechanisms and potential applications.
Journal: General psychiatry
In common: 2 references

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: code errors and mismatches between the code and the paper about this paper and its code, from readers and its authors, beside the paper and its tracing map.

Reproductions: reports from readers who ran the authors' code and did not get the paper's result, what they ran, with which environment, commit and data.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.