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Neurological Effects of <i>Cleistocalyx nervosum</i> var. <i>paniala</i> Berry on Hippocampal Transcriptome, Neuritogenesis, and Synaptogenesis.

Overview

  1. Chulalongkorn Autism Research and Innovation Center of Excellence (ChulaACE), Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; (S.K.); (T.S.)
  2. M.Sc. Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; (W.P.); (C.P.)
  3. Ph.D. Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; (T.J.); (P.L.); (P.P.); (K.K.)
  4. Biological Science and Animal Model Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand
  5. College of Oriental Medicine, Rangsit University, Pathum Thani 12000, Thailand
  6. Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand
  7. Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC 20037, USA
  8. Center of Excellence on Natural Products for Neuroprotection and Anti-Ageing, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  9. Food Toxicology Unit, Institute of Nutrition, Mahidol University, Nakhon Pathom 73170, Thailand
Institutions: Chulalongkorn University (Thailand); Mahidol University (Thailand); Rangsit University (Thailand); George Washington University (United States)
Journal: Nutrients, volume 18, issue 8, article 1200
Dates: received 3 March 2026; accepted 7 April 2026; published online 10 April 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.3390/nu18081200 · PMID 42075012 · PMCID PMC13119000 · OpenAlex W7153261440
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), rat (organism), cellular / molecular (subfield)
Methods: Statistics, Connectivity
Keywords: Cleistocalyx nervosum var. paniala, sex-specific effects, neuritogenesis, synaptogenesis, transcriptome, molecular docking
MeSH: Fruit*, Hippocampus*, Neurites*, Neurogenesis*, Plant Extracts*, Synapses*, Transcriptome*, Animals, Anthocyanins, Cells, Cultured, Female, Male, Molecular Docking Simulation, Neurodevelopment, Neurons, Neuroprotective Agents, Rats, Rats, Wistar (* major topic)
Topic: Neuroinflammation and Neurodegeneration Mechanisms (Neurology, Neuroscience), according to OpenAlex
Funding: National Research Council of Thailand (695259/2018NRCT32900); Thailand Science Research and Innovation Fund, Chulalongkorn University (HEAF67370092 and HEA_FF_68_083_3700_006); The Second Century Fund (C2F), Chulalongkorn University, Bangkok, Thailand; Chulalongkorn University Laboratory Animal Center (CULAC) (Animal Use Protocol No. 2273007); Chulalongkorn University (CE66_046_3700_003 to CE68_080_3700_001); Mahidol University (RSPG 02/2564)
Citations: not cited yet (Europe PMC); 123 references in the paper

Abstract

Background/Objectives: Neuritogenesis and synaptogenesis support learning and cognitive function, and hippocampal neurons play central roles in these processes. Cleistocalyx nervosum var. paniala (CNP), a Southeast Asian berry, has reported neuroprotective activities, but its direct effects on hippocampal neurons remain unclear. We investigated whether CNP extract modulates hippocampal neuronal transcriptomes, neuritogenesis, and synaptogenesis. Methods: Primary hippocampal neurons isolated from male and female Wistar rat pups were treated with CNP extract in vitro. Cytotoxicity was assessed to define non-cytotoxic concentrations. Transcriptomic responses were profiled by RNA sequencing and validated by RT-qPCR. Neuritogenesis was quantified by neurite morphology and Sholl analysis. Synaptogenesis was evaluated by synaptic immunocytochemistry. Molecular docking of cyanidin-3-glucoside (C3G) and resveratrol was used to generate mechanistic hypotheses. Results: At 0.1–10 µg/mL, CNP was non-cytotoxic, whereas a 100 µg/mL dose reduced viability; therefore, 10 µg/mL was used in subsequent experiments. Exploratory RNA-seq profiling identified thousands of differentially expressed genes enriched in synapse- and neurite-related pathways, including synaptogenesis signaling, axon guidance, and neuritogenesis. RT-qPCR showed upregulation of Igf1 in males and Glul in females, with sex-dependent modulation of Bdnf and Cask. CNP increased neurite length, branching, and Sholl complexity in both sexes, with a more pronounced effect in males. A male-biased effect was also observed in synapse-related marker colocalization, with increased Syn1–Psd95 colocalization detected in males. Docking suggested plausible interactions of C3G and resveratrol with regulators such as MYC, TP53, and CREB1. Conclusions: CNP extract alters transcriptional networks and enhances neurite outgrowth in primary hippocampal neurons in a sex-dependent manner, with male-biased effects on Syn1–Psd95 colocalization. These findings support further dose–response, mechanistic, and sex-stratified in vivo studies to evaluate its neurobiological potential.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data

Datasets cited

Data Availability Statement

The datasets analyzed during the current study are available in the GEO DataSets repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE284729], accessed date: 2 May 2024, and are available from the corresponding author on reasonable request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 15 authors, 6 keywords, 18 MeSH terms, 6 funders, 118 references.

Cite

This paper

Kanlayaprasit, S., Parnich, W., Jantheang, T., Lertpeerapan, P., Panjabud, P., Kasitipradit, K., Poolcharoen, C., Saeliw, T., Muangnoi, C., Plaingam, W., Charoenkiatkul, S., Hu, V. W., Tencomnao, T., Sarachana, T., & Sukprasansap, M. (2026). Neurological Effects of <i>Cleistocalyx nervosum</i> var. <i>paniala</i> Berry on Hippocampal Transcriptome, Neuritogenesis, and Synaptogenesis. Nutrients, 18(8), 1200. https://doi.org/10.3390/nu18081200

BibTeX

@article{kanlayaprasit2026neurological,
author = {Kanlayaprasit, Songphon and Parnich, Worratha and Jantheang, Thanawin and Lertpeerapan, Pattanachat and Panjabud, Pawinee and Kasitipradit, Kasidit and Poolcharoen, Chayanit and Saeliw, Thanit and Muangnoi, Chawanphat and Plaingam, Waluga and Charoenkiatkul, Somsri and Hu, Valerie W and Tencomnao, Tewin and Sarachana, Tewarit and Sukprasansap, Monruedee},
title = {{Neurological Effects of \<i\>Cleistocalyx nervosum\</i\> var. \<i\>paniala\</i\> Berry on Hippocampal Transcriptome, Neuritogenesis, and Synaptogenesis}},
journal = {Nutrients},
year = {2026},
month = apr,
volume = {18},
number = {8},
pages = {1200},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
issn = {2072-6643},
doi = {10.3390/nu18081200},
url = {https://doi.org/10.3390/nu18081200},
pmid = {42075012},
pmcid = {PMC13119000}
}

RIS

TY - JOUR
AU - Kanlayaprasit, Songphon
AU - Parnich, Worratha
AU - Jantheang, Thanawin
AU - Lertpeerapan, Pattanachat
AU - Panjabud, Pawinee
AU - Kasitipradit, Kasidit
AU - Poolcharoen, Chayanit
AU - Saeliw, Thanit
AU - Muangnoi, Chawanphat
AU - Plaingam, Waluga
AU - Charoenkiatkul, Somsri
AU - Hu, Valerie W
AU - Tencomnao, Tewin
AU - Sarachana, Tewarit
AU - Sukprasansap, Monruedee
TI - Neurological Effects of <i>Cleistocalyx nervosum</i> var. <i>paniala</i> Berry on Hippocampal Transcriptome, Neuritogenesis, and Synaptogenesis
T2 - Nutrients
J2 - Nutrients
PY - 2026
DA - 2026/04/10
VL - 18
IS - 8
SP - 1200
SN - 2072-6643
PB - Multidisciplinary Digital Publishing Institute (MDPI)
DO - 10.3390/nu18081200
UR - https://doi.org/10.3390/nu18081200
LA - en
ER -

CSL-JSON

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"language": "en",
"issued": {
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