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Dichotomy between extracellular signatures of active dendritic chemical synapses and gap junctions.

Code ↔ Paper

4 matches between paragraphs of the paper and lines of its authors' code, computed by the harvester (lexical-v1). Click a colored paragraph or line to see its counterpart.

The 4 matches · 1 of them tie a paragraph to a whole file, not to given lines: a weak match, whose lines are not tinted
  1. [1] § Methods › Analysis of extracellular field potentials ↔ Code/Matlab/Fig5.m, the whole file · a weak match · score 0.72 · Fourier transform, LFP waveform, cutoff, window, wavelet, bandpass
  2. [2] § Results › Synchronous inputs: outward transmembrane currents from active dendrites contribute to positive deflection in extracellular potentials associated with gap junctional inputs ↔ Code/Matlab/Fig1.m, lines 42–104 · score 0.62 · 190–300 um, 50–100 um, proximal electrode, 190 um, gap junctions, distal
  3. [3] § Methods › Analysis of extracellular field potentials ↔ Code/Matlab/Fig1.m, lines 4–41 · score 0.57 · bandpass filtered, cutoff, window, signals, EFP, electrodes
  4. [4] § Results › Synchronous inputs: contrasting patterns of extracellular signatures associated with active dendritic chemical synapses vs. gap junctions ↔ Code/Matlab/Fig1.m, lines 42–104 · score 0.55 · 190–300 um, 50–100 um, 190 um, gap junctions, distal, traces

Paper

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The authors' code

MATLAB · 258 lines · 8.6 KB · no license · 3 matches

  1. % GJLFP, eLife 2026. Richa Sirmaur and Rishikesh Narayanan.
  2. % Figure 1: waveforms (A-B),boxplots active vs passive (C-D), waveforms across entire neuron (E)
  3. %% Load dataset and bandpass filter EFP signals
  4. % loading the EFP data from all 7 arrays, all having 49 electrodes
  5. dataFiles = {
  6. 'S1_allLFP_E0-E48.xlsx'
  7. 'S2_allLFP_E0-E48.xlsx'
  8. 'S3_allLFP_E0-E48.xlsx'
  9. 'S4_allLFP_E0-E48.xlsx'
  10. 'S5_allLFP_E0-E48.xlsx'
  11. 'S6_allLFP_E0-E48.xlsx'
  12. 'S7_allLFP_E0-E48.xlsx'
  13. };
  14. dataRange = 'A1:AW80000';
  15. nFiles = numel(dataFiles); % seven
  16. % FILTER PARAMETERS ----------------
  17. fs = 40000; % sampling frequency (Hz)
  18. fc1 = 0.5; % low cutoff (Hz)
  19. fc2 = 300; % high cutoff (Hz)
  20. Wn = [fc1 fc2] / (fs/2); % cutoff
  21. n = 2; % filter order
  22. [b,a] = butter(n, Wn, 'bandpass');
  23. % LOAD + FILTER ----------------
  24. lfpData = [];
  25. for f = 1:nFiles
  26. Mat = xlsread(dataFiles{f}, dataRange); % Load dataset
  27. lfpMat = zeros(size(Mat)); % filtered matrix
  28. % Filter each electrode trace
  29. for i = 1:size(Mat,2)
  30. lfpMat(:,i) = filtfilt(b, a, Mat(:,i)); %forward-backward filtering for no time shift
  31. end
  32. lfpData{f} = lfpMat;
  33. end
  34. filteredmat = cat(2, lfpData{:}); % concatenating all arrays column-wise (7 arrays each with 49 electrodes) in one matrix
  35. dt = 1/fs;
  36. T = (0:size(Mat,1)-1)' * dt;
  37. % T = time(22001:34001); % specific plotting window
  38. %% A-B plot example waveforms
  39. % ####### A:
  40. basePath = '/chemsyn/synchro/passive/EFP'; % change to '../active/EFP' for active models
  41. % ===================== PROXIMAL (50–100 µm) =====================
  42. cd(fullfile(basePath, 'electrode_numbers_50-100'));
  43. % Load electrode indices
  44. q = []; % if already loaded and saved call the mat file: load('electrode_numbers.mat'); q = electrode_numbers (n=15)
  45. % combine proximal electrode data for traces
  46. data1 = [];
  47. for k = 1:numel(q)
  48. data1(:,k) = lfpMat1(:, q(k)); % arrays whose electrode numbers are proximal
  49. end
  50. data2 = [];
  51. for k = 1:numel(q)
  52. data2(:,k) = lfpMat2(:, q(k)); % arrays whose electrode numbers are proximal
  53. end
  54. LFP_prox = [data1, data2]; % Combine
  55. avg_prox = mean(LFP_prox, 2); % Average across proximal electrodes
  56. figure; % Plot
  57. hold on;
  58. for j = 1:15
  59. plot(T, LFP_prox(:,j), 'b', 'LineWidth', 1.5); % 'r' for active
  60. end
  61. plot(T, avg_prox, 'k', 'LineWidth', 3);
  62. title('LFPs: 50–100 µm (proximal- passive)');
  63. xlabel('Time');
  64. ylabel('LFP (uV)');
  65. hold off;
  66. % ===================== distal (190-300 µm) =====================
  67. cd(fullfile(basePath, 'electrode_numbers_190-300'));
  68. % Load electrode indices
  69. q = []; % load('electrode_numbers.mat'); q = electrode_numbers (n=21)
  70. % combine distal electrode data for traces
  71. data1 = [];
  72. for k = 1:numel(q)
  73. data1(:,k) = lfpMat6(:, q(k)); % arrays whose electrode numbers are picked as distal
  74. end
  75. data2 = [];
  76. for k = 1:numel(q)
  77. data2(:,k) = lfpMat7(:, q(k)); % arrays whose electrode numbers are picked as distal
  78. end
  79. LFP_dist = [data1, data2]; % Combine
  80. avg_dist = mean(LFP_dist, 2); % Average across distal electrodes
  81. figure; % Plot
  82. hold on;
  83. for j = 1:21
  84. plot(T, LFP_dist(:,j), 'b', 'LineWidth', 1.5); % 'r' for active
  85. end
  86. plot(T, avg_dist, 'k', 'LineWidth', 3);
  87. title('LFPs: 190-300 µm (distal- passive)'); % change to active
  88. xlabel('Time (ms)');
  89. ylabel('LFP (uV)');
  90. hold off;
  91. % ####### B:
  92. % SAME AS 'A' but for gap junctions: /gapj/synchro/passive/EFP, and /gapj/synchro/active/EFP
  93. %% Boxplot- active vs passive
  94. nBins = 10;
  95. activePath = '/sync/active/Negativeamps'; % files have negative amplitudes (negative peaks-baseline) from each electrodes divided into 10 bins based on radial distance from soma
  96. passivePath = '/sync/passive/Negativeamps';
  97. colors = [
  98. 190/255, 30/255, 45/255; % active
  99. 27/255, 117/255, 187/255 % passive
  100. ];
  101. activeColor = colors(1,:);
  102. passiveColor = colors(2,:);
  103. activeData = cell(nBins,1);
  104. passiveData = cell(nBins,1);
  105. for i = 1:nBins
  106. fname = sprintf('Bin%d.txt', i);
  107. activeData{i} = load(fullfile(activePath, fname));
  108. passiveData{i} = load(fullfile(passivePath, fname));
  109. end
  110. meanActive = cellfun(@mean, activeData);
  111. meanPassive = cellfun(@mean, passiveData);
  112. x = 1:nBins;
  113. xActive = x - 0.2;
  114. xPassive = x + 0.2;
  115. figure; hold on;
  116. boxWidth = 0.35;
  117. % boxplots:
  118. for i = 1:nBins
  119. % ---- ACTIVE ----
  120. boxplot(activeData{i}, 'Positions', xActive(i), ...
  121. 'Widths', boxWidth, ...
  122. 'Colors', activeColor, ...
  123. 'Symbol', '');
  124. h = findobj(gca,'Tag','Box');
  125. patch(get(h(1),'XData'), get(h(1),'YData'), ...
  126. activeColor, 'FaceAlpha', 0.2, ...
  127. 'EdgeColor', activeColor, 'LineWidth', 2);
  128. % ---- PASSIVE ----
  129. boxplot(passiveData{i}, 'Positions', xPassive(i), ...
  130. 'Widths', boxWidth, ...
  131. 'Colors', passiveColor, ...
  132. 'Symbol', '');
  133. h = findobj(gca,'Tag','Box');
  134. patch(get(h(1),'XData'), get(h(1),'YData'), ...
  135. passiveColor, 'FaceAlpha', 0.2, ...
  136. 'EdgeColor', passiveColor, 'LineWidth', 2);
  137. end
  138. % set(findobj(gca,'Type','line'),'LineStyle','-');
  139. xticks(linspace(0,250,6));
  140. xticklabels(string(linspace(0,250,6)));
  141. xlabel('Radial distance (µm)');
  142. ylabel('LFP');
  143. title('Active vs Passive LFP');
  144. set(gca, 'YScale', 'log');
  145. box off;
  146. hold off;
  147. % Inset: line plot:
  148. fogure; hold on;
  149. plot(x, meanActive, '-', ...
  150. 'Color', activeColor, ...
  151. 'LineWidth', 2);
  152. plot(x, meanPassive, '-', ...
  153. 'Color', passiveColor, ...
  154. 'LineWidth', 2);
  155. set(gca, 'XLim', [0 250]);
  156. box off;
  157. hold off;
  158. %% waveforms spanning the entire neuron (E)
  159. % basal waveforms from above mentioned filtered mat, load and filter EFP recorded from apical dendritic side
  160. dataFiles_apical = {
  161. 'S1_allLFP_E0-E48.xlsx'
  162. 'S2_allLFP_E0-E48.xlsx'
  163. 'S3_allLFP_E0-E48.xlsx'
  164. 'S4_allLFP_E0-E48.xlsx'
  165. 'S5_allLFP_E0-E48.xlsx'
  166. 'S6_allLFP_E0-E48.xlsx'
  167. 'S7_allLFP_E0-E48.xlsx'
  168. };
  169. dataRange = 'A1:AW80000';
  170. nFiles = numel(dataFiles); % seven
  171. lfpData_apical = [];
  172. for f = 1:nFiles
  173. Mat_apical = xlsread(dataFiles_apical{f}, dataRange); % Load dataset
  174. lfpMat = zeros(size(Mat_apical)); % filtered matrix
  175. % Filter each electrode trace
  176. for i = 1:size(Mat_apical,2)
  177. lfpMat(:,i) = filtfilt(b, a, Mat_apical(:,i)); %forward-backward filtering for no time shift
  178. end
  179. lfpData_apical{f} = lfpMat_apical;
  180. end
  181. filteredmat_apical = cat(2, lfpData_apical{:}); % concatenating all arrays column-wise (7 arrays each with 49 electrodes) in one matrix
  182. T = T(4001:48001,1);
  183. % basal:
  184. waveform_1 = FilteredMat2(4001:48001,32); waveforms(:,1) = waveform_1;
  185. waveform_2 = FilteredMat3(4001:48001,32); waveforms(:,2) = waveform_2;
  186. waveform_3 = FilteredMat3(4001:48001,39); waveforms(:,3) = waveform_3;
  187. waveform_4 = FilteredMat1(4001:48001,34); waveforms(:,4) = waveform_4;
  188. waveform_5 = FilteredMat3(4001:48001,45); waveforms(:,5) = waveform_5;
  189. waveform_6 = FilteredMat1(4001:48001,36); waveforms(:,6) = waveform_6;
  190. waveform_7 = FilteredMat6(4001:48001,47); waveforms(:,7) = waveform_7;
  191. % waveform_8 = FilteredMat2(4001:48001,4); waveforms(:,8) = waveform_8;
  192. % waveform_9 = FilteredMat3(4001:48001,1); waveforms(:,9) = waveform_9;
  193. % waveform_10 = FilteredMat6(4001:48001,7); waveforms(:,10) = waveform_10;
  194. % apical
  195. Ap_waveforms_A = zeros(length(T), num_Ap_waveforms);
  196. Ap_waveform_11 = filteredmat_apical2(4001:48001,32); Ap_waveforms_A(:,1) = Ap_waveform_11;
  197. Ap_waveform_12 = filteredmat_apical3(4001:48001,32); Ap_waveforms_A(:,2) = Ap_waveform_12;
  198. Ap_waveform_13 = filteredmat_apical3(4001:48001,39); Ap_waveforms_A(:,3) = Ap_waveform_13;
  199. Ap_waveform_14 = filteredmat_apical1(4001:48001,34); Ap_waveforms_A(:,4) = Ap_waveform_14;
  200. Ap_waveform_15 = filteredmat_apical3(4001:48001,45); Ap_waveforms_A(:,5) = Ap_waveform_15;
  201. Ap_waveform_16 = filteredmat_apical1(4001:48001,36); Ap_waveforms_A(:,6) = Ap_waveform_16;
  202. Ap_waveform_17 = filteredmat_apical6(4001:48001,47); Ap_waveforms_A(:,7) = Ap_waveform_17;
  203. % Ap_waveform_18 = filteredmat_apical2(4001:48001,4); Ap_waveforms_A(:,8) = Ap_waveform_18;
  204. % Ap_waveform_19 = filteredmat_apical3(4001:48001,1); Ap_waveforms_A(:,9) = Ap_waveform_19;
  205. % Ap_waveform_20 = filteredmat_apical6(4001:48001,7); Ap_waveforms_A(:,10) = Ap_waveform_20;
  206. figure;
  207. num_waveforms = 7; % Number of waveforms in each set (taking 7 for plotting)
  208. total_waveforms = 14; % Total number
  209. for i = 1:total_waveforms
  210. subplot(total_waveforms, 1, i);
  211. if i <= num_waveforms
  212. % Plot the first 7 waveforms from waveforms (basal)
  213. plot(T, waveforms(:,i));
  214. else
  215. % Plot the next 7 waveforms from Ap_waveforms (apical)
  216. plot(T, Ap_waveforms_(:,i - num_waveforms));
  217. end
  218. xlabel('Time');
  219. ylabel('Amplitude');
  220. % title(['Waveform ', num2str(i)]); % Uncomment this line if you want to add titles
  221. % xlim([100 1200]);
  222. % ylim([-100 100]);
  223. box off;
  224. end
  225. % do the same for gap junctional EFPs for right side waveforms.

Fig1.m, no license · at the source

Overview

  1. Cellular Neurophysiology Laboratory, Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
Journal: eLife, volume 14, article RP103046
Dates: published online 30 July 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.7554/elife.103046 · PMID 42528398 · PMCID PMC13423354 · OpenAlex W4407969355
Open access: gold, a free copy (OpenAlex)
Status: code verified
Methods: Spectral & time-frequency, Statistics, Smoothing, state filtering, decompositions, Machine learning, Preprocessing
Keywords: None
MeSH: Dendrites*, Gap Junctions*, Synapses*, Action Potentials, Animals, Local Field Potential Measurement (* major topic)
Topic: Neuroscience and Neuropharmacology Research (Cellular and Molecular Neuroscience, Neuroscience), according to OpenAlex
Funding: DBT/Wellcome Trust India Alliance (Senior fellowship IA/S/16/2/502727, IA/S/16/2/502727); Ministry of Education, India (Scholarship and research funds)
Citations: not cited yet (Europe PMC); 129 references in the paper
Research resources: Igor Pro RRID:SCR_000325, MATLAB RRID:SCR_001622, R Project for Statistical Computing RRID:SCR_001905, NEURON RRID:SCR_005393, Python Programming Language RRID:SCR_008394, LFPy RRID:SCR_014805, Jupyter Notebook RRID:SCR_018315

Abstract

Local field potentials (LFPs) are compound signals that represent the dynamic flow of information across the brain, which have been historically associated with chemical synaptic inputs. How do gap junctional inputs onto active compartments shape LFPs? We developed a methodology to record extracellular potentials associated with different patterns of gap junctional inputs onto conductance-based models. We found that synchronous inputs through chemical synapses yielded a negative deflection in proximal extracellular electrodes whereas those onto gap junctions manifested a positive deflection. Importantly, we observed extracellular dipoles only when inputs arrived through chemical synapses but not with gap junctions. Remarkably, hyperpolarization-activation cyclic nucleotide-gated channels, which typically conduct inward currents, mediated outward currents triggered by the fast voltage transition caused by synchronous inputs. With rhythmic inputs at different frequencies arriving through gap junctions, we found strong suppression of LFP power at higher frequencies as well as frequency-dependent differences in the spike phase associated with the LFP when compared to respective chemical synaptic counterparts. All observed differences in LFP were mediated by the relative dominance of synaptic currents vs. voltage-driven transmembrane currents with chemical synapses vs. gap junctions, respectively. Our analyses unveil a hitherto unknown role for active dendritic gap junctions in shaping extracellular potentials.

Reproduced under the paper's license (CC BY), from the paper cited above.

Repositories

Its files are read in the Code ↔ Paper reader above, with 4 matches between paragraphs and lines of code.

Zenodo 21312084

License: CC-BY-4.0
State: the link answers, verified on 27 September 2026
Evidence: files inventoried
Size: 1 file
Software Heritage: not checked
Found in: “Data availability”
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Tools: NEURON (50 files), Signal Processing Toolbox (7 files), LFPy (6 files), Matplotlib (6 files), NumPy (6 files), Statistics and Machine Learning Toolbox (2 files), Wavelet Toolbox (2 files)
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
  • 27 September 2026: the link answers (HTTP 200)
58 files

supp:PMC13423354/elife-103046-code1.zip

License: none: the authors keep all their rights
State: the link answers, verified on 27 September 2026
Evidence: files inventoried
Languages: NEURON (44), C (36), MATLAB (8), Python (6), C++ (2)
Size: 96 files, 96 scripts
Software Heritage: not checked
Found in: the supplementary material
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Tools: NEURON (50 files), Signal Processing Toolbox (7 files), LFPy (6 files), Matplotlib (6 files), NumPy (6 files), Statistics and Machine Learning Toolbox (2 files), Wavelet Toolbox (2 files)
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
  • 27 September 2026: the link answers (HTTP 200)
96 files

The paper's code and data availability statement is in the Data section.

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  • 2 repositories of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 154 scripts, each with its path and the digest of its content;
  • 4 matches between paragraphs of the paper and lines of the code (method lexical-v1);
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Data availability

The current manuscript is a computational study, so no data have been generated for this manuscript. All source codes for simulation and analyses are publicly available at https://doi.org/10.5281/zenodo.21312084.

The following dataset was generated:

Sirmaur R, Narayanan R. 2026. Codes. Zenodo.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 2 authors, 1 keyword, 6 MeSH terms, 2 funders, 127 references, 7 RRIDs.

Cite

This paper

Sirmaur, R., & Narayanan, R. (2026). Dichotomy between extracellular signatures of active dendritic chemical synapses and gap junctions. eLife, 14, RP103046. https://doi.org/10.7554/elife.103046

BibTeX

@article{sirmaur2026dichotomy,
author = {Sirmaur, Richa and Narayanan, Rishikesh},
title = {{Dichotomy between extracellular signatures of active dendritic chemical synapses and gap junctions}},
journal = {eLife},
year = {2026},
month = jul,
volume = {14},
pages = {RP103046},
publisher = {eLife Sciences Publications, Ltd},
issn = {2050-084X},
doi = {10.7554/elife.103046},
url = {https://doi.org/10.7554/elife.103046},
pmid = {42528398},
pmcid = {PMC13423354}
}

RIS

TY - JOUR
AU - Sirmaur, Richa
AU - Narayanan, Rishikesh
TI - Dichotomy between extracellular signatures of active dendritic chemical synapses and gap junctions
T2 - eLife
J2 - eLife
PY - 2026
DA - 2026/07/30
VL - 14
SP - RP103046
SN - 2050-084X
PB - eLife Sciences Publications, Ltd
DO - 10.7554/elife.103046
UR - https://doi.org/10.7554/elife.103046
LA - en
ER -

CSL-JSON

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"title": "Dichotomy between extracellular signatures of active dendritic chemical synapses and gap junctions",
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"container-title-short": "eLife",
"volume": "14",
"page": "RP103046",
"DOI": "10.7554/elife.103046",
"PMID": "42528398",
"PMCID": "PMC13423354",
"ISSN": "2050-084X",
"publisher": "eLife Sciences Publications, Ltd",
"URL": "https://doi.org/10.7554/elife.103046",
"language": "en",
"issued": {
"date-parts": [
[
2026,
7,
30
]
]
}
}

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