DSP1 regulates planarian (Dugesia japonica) neural regeneration depending on CnB

Overview

Authors: Lili Gao¹, Mingyue Zheng², Ziyuzhu Kong¹, Yutong Li¹, Jiaxin Li¹, Fengtang Yang¹, Zhonghong Cao¹, Hui Zhen¹

School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China
School of Medical Laboratory, Qilu Medical University, Zibo, China

Journal: Frontiers in cell and developmental biology, volume 14, article 1932725

Dates: received 9 July 2026; accepted 25 September 2026; published online 8 October 2026

Type: Research article · Language: English

License: CC BY

Identifiers: DOI · PMCID PMC13688025

Status: data only (how the code is found and verified)

Categories: other (organism), cellular / molecular (subfield)

Methods: Statistics, Evoked potentials

Keywords: CnB, DSP1, Dugesia japonica, neural regeneration, regulate

Citations: not cited yet (Europe PMC); 41 references in the paper

Abstract

Regeneration and repair of the injured central nervous system is a major focus in biological research, yet many key questions remain unanswered, making it essential to explore the mechanisms underlying neural regeneration. Dorsal switch protein 1 (DSP1) plays critical roles in embryonic development and immune regulation in multiple invertebrates including Drosophila, whereas its vertebrate ortholog HMGB1 participates in diverse biological processes; however, its function in central nervous system regeneration has not been reported. In this study, we found that DSP1 was widely expressed in the intact planarian, predominantly in parenchyma, retropharyngeal and cephalic ganglia. Knockdown of DSP1 promoted blastema growth at one dpa, without affecting the stem cell population. Silencing of DSP1 accelerated brain regeneration and significantly increased the number of daughter cells derived from stem cells, accompanied by downregulated expression of calcineurin subunit B (CnB), a Wnt signaling pathway gene. Notably, simultaneous knockdown of DSP1 and CnB promoted blastema regeneration. These findings suggest DSP1 is associated with CnB to regulate neural regeneration in planarian. These findings provide a theoretical basis for understanding the DSP1/CnB signaling axis in regulating neural regeneration in mammals, and have important implications for the development of drugs targeting neuronal regeneration.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

Datasets cited

bioproject:PRJNA1483247, at NCBI BioProject; found in “Data availability statement”

Data availability statement

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1483247 https://www.researchsquare.com/article/rs-9686603/v1, DOI: https://doi.org/10.21203/rs.3.rs-9686603/v1.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 1, 24 October 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 8 authors, 5 keywords, 41 references.

Cite

This paper

Gao, L., Zheng, M., Kong, Z., Li, Y., Li, J., Yang, F., Cao, Z., & Zhen, H. (2026). DSP1 regulates planarian (Dugesia japonica) neural regeneration depending on CnB. Frontiers in cell and developmental biology, 14, 1932725.

BibTeX

@article{gao2026dsp1, author = {Gao, Lili and Zheng, Mingyue and Kong, Ziyuzhu and Li, Yutong and Li, Jiaxin and Yang, Fengtang and Cao, Zhonghong and Zhen, Hui}, title = {{DSP1 regulates planarian (Dugesia japonica) neural regeneration depending on CnB}}, journal = {Frontiers in cell and developmental biology}, year = {2026}, month = oct, volume = {14}, pages = {1932725}, publisher = {Frontiers Media SA}, issn = {2296-634X}, pmcid = {PMC13688025} }

RIS

TY - JOUR AU - Gao, Lili AU - Zheng, Mingyue AU - Kong, Ziyuzhu AU - Li, Yutong AU - Li, Jiaxin AU - Yang, Fengtang AU - Cao, Zhonghong AU - Zhen, Hui TI - DSP1 regulates planarian (Dugesia japonica) neural regeneration depending on CnB T2 - Frontiers in cell and developmental biology J2 - Front Cell Dev Biol PY - 2026 DA - 2026/10/24 VL - 14 SP - 1932725 SN - 2296-634X PB - Frontiers Media SA LA - en ER -

CSL-JSON

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