Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils.
Overview
- Department of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany
- Department of Dermatology, University Hospital Münster, Münster, Germany
- Institute of Physical Chemistry, Göttingen University, Göttingen, Germany
- Department of Dermatology, Venereology and Allergology, University Medical Center, Göttingen University, Göttingen, Germany
- Molecular Physiology, Institute of Cardiovascular Physiology, University Medical Center, Georg‐August‐University, Göttingen, Germany
- Department of Molecular Neurobiology, Max Planck Institute For Multidisciplinary Sciences, Göttingen, Germany
- ZEMOS Center for Solvation Science Ruhr University Bochum, Bochum, Germany
- Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Münster, Germany
- Institute For Immunology, Heidelberg University Hospital Heidelberg, Heidelberg, Germany
- Biomedical Nanosensors, Fraunhofer Institute for Microelectronic Circuits and Systems, Duisburg, Germany
Abstract
Neutrophils are key inflammatory effector cells and rapidly integrate chemical signals during inflammation. They have long been suspected to use catecholamines (CAs) as immunoregulatory signals, but direct evidence for CA handling in these cells has been lacking due to the absence of suitable real‐time detection tools. Here, we combine fluorescent false neurotransmitters (FFNs), near‐infrared fluorescent single‐walled carbon nanotube (SWCNT)‐based catecholamine nanosensors, dual‐color Ca2 + imaging, and transcriptomics to resolve neutrophil CA dynamics with high spatiotemporal precision. Using FFNs, we demonstrate VMAT2‐dependent vesicular uptake of catecholamines within seconds as well as CA transfer between cells. Serotonin, LPS, and activated platelets trigger calcium (Ca2+) signaling and subsequent fast transient release of CAs from neutrophils, which we directly visualize in real time using SWCNT‐based nanosensors. In human experimental endotoxemia, longitudinal transcriptomics reveal coordinated regulation of monoaminergic receptors, synthesis machinery, and transporters, suggesting adaptive tuning of neutrophils to inflammatory CA exposure. CAs suppress NET formation but enhance thrombin‐induced platelet aggregation, and serotonin‐dependent platelet–neutrophil interactions evoke CA release, establishing a paracrine feedback loop linking inflammation and coagulation. Our integrated imaging and sensing workflow provides direct evidence for rapid vesicular catecholamine communication in human neutrophils and uncovers previously unrecognized mechanistic parallels between neurons and neutrophils. It offers a broadly applicable platform to interrogate monoamine signaling in immune cells.
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
The paper links to its data, not to its authors' code: see the Data section.
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Data
Datasets cited
- geo:GSE35590, at NCBI GEO; found in the text, “Transcriptomics”
Data Availability Statement
All data are available in the main text or the supplementary materials. Raw data will be uploaded to a repository once the paper has been accepted.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 20 authors, 5 keywords, 8 MeSH terms, 2 funders, 67 references, 3 RRIDs.
Cite
This paper
Mohr, J., Schmitz, A., Dinarvand, M., Wulfert, F., Shankar, S., Hill, B. F., Wojak, M., Gretz, J., Britz, M., Neubert, E., Shumanska, M., Kaushik, S., Kartaschew, L., Bogeski, I., Daniel, J., Jung, S., Eble, J., Wabnitz, G., Erpenbeck, L., & Kruss, S. (2026). Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 13(47), e24193. https://
BibTeX
@article{mohr2026multimo
author = {Mohr, Jennifer and Schmitz, Anne and Dinarvand, Meshkat and Wulfert, Franziska and Shankar, Sangeetha and Hill, Bjoern F and Wojak, Michael and Gretz, Juliana and Britz, Marie and Neubert, Elsa and Shumanska, Magdalena and Kaushik, Sofia and Kartaschew, Linda and Bogeski, Ivan and Daniel, James and Jung, Sebastian and Eble, Johannes and Wabnitz, Guido and Erpenbeck, Luise and Kruss, Sebastian},
title = {{Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils}},
journal = {Advanced science (Weinheim, Baden-Wurttemberg, Germany)},
year = {2026},
month = jun,
volume = {13},
number = {47},
pages = {e24193},
publisher = {Wiley},
issn = {2198-3844},
doi = {10.1002/
url = {https://
pmid = {42314056},
pmcid = {PMC13336083}
}
RIS
TY - JOUR
AU - Mohr, Jennifer
AU - Schmitz, Anne
AU - Dinarvand, Meshkat
AU - Wulfert, Franziska
AU - Shankar, Sangeetha
AU - Hill, Bjoern F
AU - Wojak, Michael
AU - Gretz, Juliana
AU - Britz, Marie
AU - Neubert, Elsa
AU - Shumanska, Magdalena
AU - Kaushik, Sofia
AU - Kartaschew, Linda
AU - Bogeski, Ivan
AU - Daniel, James
AU - Jung, Sebastian
AU - Eble, Johannes
AU - Wabnitz, Guido
AU - Erpenbeck, Luise
AU - Kruss, Sebastian
TI - Multimodal Imaging Reveals Rapid Catecholamine Uptake and Release by Neutrophils
T2 - Advanced science (Weinheim, Baden-Wurttemberg, Germany)
J2 - Adv Sci (Weinh)
PY - 2026
DA - 2026/
VL - 13
IS - 47
SP - e24193
SN - 2198-3844
PB - Wiley
DO - 10.1002/
UR - https://
LA - en
ER -
CSL-JSON
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