RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System.
Overview
- Whitman Center Marine Biological Laboratory Woods Hole Massachusetts USA
- Institute of Biochemistry Charité‐Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Berlin and Berlin Institute of Health & Laboratory of Molecular Neuroscience German Center for Neurodegenerative Diseases (DZNE) Berlin Germany
- Institute of Biology Freie Universität Berlin Berlin Germany
- Department of Cell Biology Duke University Durham North Carolina USA
- Human Biology and Primate Evolution Freie Universität Berlin Berlin Germany
- Max Planck Institute for Molecular Cell Biology and Genetics Dresden Germany
- Department of Molecular Immunology Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen The Netherlands
- Department of Neuropathology Charité ‐ Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Zu Berlin and Berlin Institute of Health & German Center for Neurodegenerative Diseases (DZNE) Berlin Germany
- Goethe University Frankfurt Institute For Molecular Biosciences Frankfurt am Main Germany
- Institute For Neuro‐ and Sensory Physiology University Medical Center Göttingen Göttingen Germany
- Einstein Center for Neuroscience Charité‐Universitätsmedizin Berlin Corporate Member of Freie Universität Berlin Humboldt‐Universität Berlin and Berlin Institute of Health Berlin Germany
Abstract
Condensates at synapses organize synaptic vesicle (SV) clusters and are essential for efficient neurotransmitter release. While it is established that RNA granules traffic along axons, the function of RNA at the presynapse remains unclear. Here, we uncover a direct structural role of coding RNAs in organizing presynaptic condensates by focusing on SV clusters, condensates between synapsin‐1 and lipid vesicles. Using in vitro reconstitution systems, we show that RNA drives synapsin‐1 coacervation, with structured RNAs being more effective at promoting phase transitions. The importance of RNA was confirmed in living synapses, where acute disruption of native RNA induces a dispersion of SVs and synapsin. Conversely, ectopically expressed SV‐like condensates contained the translational machinery. The microscopy‐based in vitro translation assay demonstrates increased translation efficiency within synapsin‐1/
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
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All codes written for this study are available upon reasonable request from the corresponding authors.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data Availability Statement
Source data are provided with this paper. All data generated or analyzed for this study are available within the paper and its associated supplementary information files. All other data presented are available upon reasonable request from the corresponding authors.
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Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, pages, dates, 22 authors, 7 keywords, 8 funders, 99 references.
Cite
This paper
Rankovic, B., Geisterfer, Z. M., Chhabra, A., Jovanovic, V. M., Seim, I., Hoffmann, C., Condric, A., Aguilar Pérez, G., de Boer, R., Hofstede, L. T., Sun, J., Freitag, K., Nowick, K., Jendrach, M., Heppner, F. L., Despic, V., Müller‐McNicoll, M., Sigrist, S., van den Bogaart, G., . . . Milovanovic, D. (2026). RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System. Advanced science (Weinheim, Baden-Wurttemberg, Germany), e77115. https://
BibTeX
@article{rankovic2026rna
author = {Rankovic, Branislava and Geisterfer, Zachary M. and Chhabra, Akshita and Jovanovic, Vladimir M. and Seim, Ian and Hoffmann, Christian and Condric, Antonela and Aguilar Pérez, Gerard and de Boer, Rinse and Hofstede, Lars T. and Sun, Jingao and Freitag, Kiara and Nowick, Katja and Jendrach, Marina and Heppner, Frank L. and Despic, Vladimir and Müller‐McNicoll, Michaela and Sigrist, Stephan and van den Bogaart, Geert and Reshetniak, Sofiia and Gladfelter, Amy S. and Milovanovic, Dragomir},
title = {{RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System}},
journal = {Advanced science (Weinheim, Baden-Wurttemberg, Germany)},
year = {2026},
month = aug,
pages = {e77115},
publisher = {Wiley},
issn = {2198-3844},
doi = {10.1002/
url = {https://
pmid = {42627687},
pmcid = {PMC13496291}
}
RIS
TY - JOUR
AU - Rankovic, Branislava
AU - Geisterfer, Zachary M.
AU - Chhabra, Akshita
AU - Jovanovic, Vladimir M.
AU - Seim, Ian
AU - Hoffmann, Christian
AU - Condric, Antonela
AU - Aguilar Pérez, Gerard
AU - de Boer, Rinse
AU - Hofstede, Lars T.
AU - Sun, Jingao
AU - Freitag, Kiara
AU - Nowick, Katja
AU - Jendrach, Marina
AU - Heppner, Frank L.
AU - Despic, Vladimir
AU - Müller‐McNicoll, Michaela
AU - Sigrist, Stephan
AU - van den Bogaart, Geert
AU - Reshetniak, Sofiia
AU - Gladfelter, Amy S.
AU - Milovanovic, Dragomir
TI - RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System
T2 - Advanced science (Weinheim, Baden-Wurttemberg, Germany)
J2 - Adv Sci (Weinh)
PY - 2026
DA - 2026/
SP - e77115
SN - 2198-3844
PB - Wiley
DO - 10.1002/
UR - https://
LA - en
ER -
CSL-JSON
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