MiR-30a-5p mediates epileptogenesis in epilepsy models by targeting SOX4 to regulate the Wnt/β-catenin pathway.
The 1 match
- [1] § RESULTS › Bioinformatics prediction indicates that miR‐30a‐5p participates in the pathogenesis of epilepsy by regulating the Wnt/β‐catenin signaling pathway through targeting SOX4 ↔ EPI4-11-983-s003.r, lines 207–255 · score 0.57 · Cellular components, Molecular functions, Biological processes, pathway, KEGG
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The authors' code
R · 255 lines · 10 KB · no license · 1 match
- # 安装必要的包(如果未安装)
- if (!require("BiocManager", quietly = TRUE))
- install.packages("BiocManager")
- required_packages <- c("clusterProfiler", "org.Hs.eg.db", "enrichplot",
- "ggplot2", "DOSE", "stringr", "dplyr", "tidyr")
- for (pkg in required_packages) {
- if (!require(pkg, character.only = TRUE)) {
- BiocManager::install(pkg)
- }
- library(pkg, character.only = TRUE)
- }
- # 读取基因列表(从你的数据)
- # 假设你的基因列表在一个名为"genes.txt"的文件中,每行一个基因
- # 或者你可以直接使用向量
- # 这里我创建了基因列表向量(从你提供的数据)
- gene_list <- c(
- "TWF1", "KLHL20", "PPARGC1B", "MKRN3", "B3GNT5", "RORA", "FZD3", "BRWD3", "CELSR3",
- "XPO1", "PDE7A", "NT5E", "EED", "RFX6", "RFX7", "KLHL28", "TNRC6A", "SCN9A", "CTNNB1",
- "MTDH", "NEDD4", "CHD1", "LMBR1", "PLAGL2", "FRZB", "SETD5", "SOX9", "EML1", "REEP3",
- "RUNX1", "FOXG1", "STK39", "VIM", "KLF10", "NFIB", "SPEN", "SH2B3", "NECAP1", "STIM2",
- "ITGA6", "RUNX2", "OTUD6B", "SAMD8", "SOCS1", "GOLGA1", "AZIN1", "CNKSR2", "CFL2",
- "PFN2", "COL13A1", "SOCS3", "ANKRD17", "EEA1", "CLOCK", "TNRC6B", "SEC23A", "TMOD2",
- "RAB8A", "WNT3A", "PSMD7", "BNIP3L", "SH3RF1", "RAPGEF4", "RAP1B", "CAMK2D", "LRRC8C",
- "KMT2C", "PAWR", "PGM1", "LPP", "TAOK1", "SCN1A", "PRLR", "ERLIN1", "SACS", "OXR1",
- "OSBPL8", "VPS26B", "PICALM", "ZNF711", "PPARGC1A", "MCF2L", "RASA1", "CAND1", "GATM",
- "PER2", "SIX4", "LRRC8D", "ARHGEF6", "MYO5A", "CALU", "PRKAA2", "DNMT3A", "EDNRA",
- "TENM3", "CBX2", "STX16", "ACTR1A", "NRIP1", "RAB23", "PDE4D", "ABL1", "LOX", "EPG5",
- "VAT1", "NDEL1", "ZDHHC17", "RHOB", "DLGAP1", "MAP3K5", "OSTM1", "SLC25A36", "PPP3CA",
- "FLVCR1", "CHD7", "EDEM3", "TMEM87A", "SNX10", "ELAVL2", "HOXA1", "NAA25", "PPP1R12A",
- "GJA1", "BCL9", "IDH1", "KMT2A", "PIP4K2B", "HIPK2", "MAML1", "ACVR1", "BCOR", "DDIT4",
- "ARID1A", "SLC38A2", "GLI2", "GALNT2", "DOCK7", "ATXN1", "ATP2A2", "SNAI1", "CSNK1G1",
- "NF1", "DSTYK", "CADPS", "ELOVL5", "GOLGA4", "IRF4", "NR4A2", "JOSD1", "NLGN1", "EFNA3",
- "CHMP2B", "PGM3", "IFNAR2", "RNF220", "SLC9A8", "PGP", "ST8SIA4", "IRS1", "ITGB3",
- "LRP6", "ZBTB18", "REEP1", "EPB41", "LIFR", "DNAJC13", "PDCD10", "INPP4A", "AP4E1",
- "MIA3", "EDC3", "DPYSL2", "UBE3C", "GCLC", "PI4K2B", "SLC4A7", "NUS1", "CAMK4", "PDGFRB",
- "RAPGEF2", "RTN4R", "TBC1D2B", "STAG2", "MAPK8", "SP4", "MAP3K7", "TRIO", "FAM13A",
- "ATP2B1", "ATL2", "GRB10", "PRPF40A", "CRKL", "IGF2R", "STT3B", "SLC5A3", "ZEB2",
- "PIGA", "TMCC1", "EPC2", "SLC6A6", "FIGN", "KCTD5", "TRAF3", "SBF1", "CADM2", "RASGEF1B",
- "NR3C1", "NEDD4L", "NAPG", "B4GALT6", "KRAS", "PAX9", "TNPO3", "RPS6KA2", "USP45",
- "LEPR", "SIK3", "AGO1", "ASCC3", "SLC7A6", "HNRNPC", "ATP2B2", "GCNT2", "QKI", "ITSN1",
- "MTTP", "CLCF1", "UGT8", "GALNT3", "AP3S1", "FXR1", "PCDH10", "HNRNPA2B1", "USP2",
- "MBNL1", "SALL4", "CPEB4", "LRCH2", "KCTD20", "BTBD10", "SLC6A9", "PHF6", "PLXNA2",
- "FAM217B", "TET1", "WDR26", "PPFIA2", "AFF3", "HNRNPA3", "IRS2", "MBNL2", "SETD3",
- "SLC7A11", "PPIP5K2", "SOS1", "WDFY3", "SEC23IP", "KCTD3", "SLC35A5", "TRPS1", "FYCO1",
- "TNKS", "NFIA", "ARF4", "SNAPIN", "LCOR", "LARP4", "UBE2D3", "HERC2", "JARID2", "ALPK3",
- "ZNF746", "GLUD1", "ZFYVE26", "NID1", "PBRM1", "PLXNA1", "SLC38A1", "SATB1", "DGKZ",
- "USP22", "UNKL", "PGM2L1", "CEP170B", "SON", "ZNF148", "MAN1B1", "ZBTB7A", "SIRT1",
- "MTF2", "YES1", "NCOA3", "SOX4", "RAB10", "SLC29A3", "H6PD", "NOVA1", "RNMT", "ACTN1",
- "NSD1", "CCNY", "PITX1", "JAG2", "USP15", "BAZ1A", "AGO2", "ADAM10", "MINPP1", "MIB1",
- "TIMP2", "TXNDC5", "SNX27", "FUCA1", "CHKA", "FOXP4", "SUCLG2", "CHD9", "GNAQ", "TBL1X",
- "APC", "RAB11A", "TIA1", "EPB41L4B", "DOC2A", "FRMD4A", "MMP9", "Axin 2", "CTH", "MECP2",
- "SMARCD2", "Cyclin D1", "TUBGCP3", "Axin 1"
- )
- # 注意:基因符号需要标准化(去除空格等)
- gene_list <- gsub(" ", "", gene_list) # 去除空格
- gene_list <- unique(gene_list) # 去重
- cat("基因列表数量:", length(gene_list), "\n")
- # 将基因符号转换为ENTREZID
- gene_entrez <- bitr(gene_list,
- fromType = "SYMBOL",
- toType = "ENTREZID",
- OrgDb = org.Hs.eg.db)
- cat("成功转换的基因数量:", nrow(gene_entrez), "\n")
- if (nrow(gene_entrez) == 0) {
- stop("没有基因被成功转换,请检查基因符号是否正确")
- }
- # 1. GO富集分析
- go_bp <- enrichGO(gene = gene_entrez$ENTREZID,
- OrgDb = org.Hs.eg.db,
- keyType = "ENTREZID",
- ont = "BP", # BP:生物过程,MF:分子功能,CC:细胞组分
- pvalueCutoff = 0.05,
- qvalueCutoff = 0.2,
- readable = TRUE)
- go_mf <- enrichGO(gene = gene_entrez$ENTREZID,
- OrgDb = org.Hs.eg.db,
- keyType = "ENTREZID",
- ont = "MF",
- pvalueCutoff = 0.05,
- qvalueCutoff = 0.2,
- readable = TRUE)
- go_cc <- enrichGO(gene = gene_entrez$ENTREZID,
- OrgDb = org.Hs.eg.db,
- keyType = "ENTREZID",
- ont = "CC",
- pvalueCutoff = 0.05,
- qvalueCutoff = 0.2,
- readable = TRUE)
- # 2. KEGG富集分析
- kegg <- enrichKEGG(gene = gene_entrez$ENTREZID,
- organism = 'hsa', # 人类
- pvalueCutoff = 0.05,
- qvalueCutoff = 0.2)
- # 将KEGG结果转换为可读的基因符号
- if (!is.null(kegg)) {
- kegg <- setReadable(kegg, OrgDb = org.Hs.eg.db, keyType = "ENTREZID")
- }
- # 保存结果到文件
- output_dir <- "enrichment_results"
- if (!dir.exists(output_dir)) {
- dir.create(output_dir)
- }
- # 保存GO结果
- if (nrow(go_bp) > 0) {
- write.csv(go_bp, file.path(output_dir, "GO_BP_enrichment.csv"), row.names = FALSE)
- cat("GO BP结果已保存到", file.path(output_dir, "GO_BP_enrichment.csv"), "\n")
- }
- if (nrow(go_mf) > 0) {
- write.csv(go_mf, file.path(output_dir, "GO_MF_enrichment.csv"), row.names = FALSE)
- cat("GO MF结果已保存到", file.path(output_dir, "GO_MF_enrichment.csv"), "\n")
- }
- if (nrow(go_cc) > 0) {
- write.csv(go_cc, file.path(output_dir, "GO_CC_enrichment.csv"), row.names = FALSE)
- cat("GO CC结果已保存到", file.path(output_dir, "GO_CC_enrichment.csv"), "\n")
- }
- # 保存KEGG结果
- if (!is.null(kegg) && nrow(kegg) > 0) {
- write.csv(kegg, file.path(output_dir, "KEGG_enrichment.csv"), row.names = FALSE)
- cat("KEGG结果已保存到", file.path(output_dir, "KEGG_enrichment.csv"), "\n")
- }
- # 3. 可视化
- # 创建可视化目录
- vis_dir <- file.path(output_dir, "visualization")
- if (!dir.exists(vis_dir)) {
- dir.create(vis_dir)
- }
- # GO富集条形图(BP为例)
- if (nrow(go_bp) > 0) {
- pdf(file.path(vis_dir, "GO_BP_barplot.pdf"), width = 12, height = 8)
- print(barplot(go_bp, showCategory = 20, title = "GO Biological Process Enrichment"))
- dev.off()
- # 点图
- pdf(file.path(vis_dir, "GO_BP_dotplot.pdf"), width = 12, height = 8)
- print(dotplot(go_bp, showCategory = 20, title = "GO Biological Process Enrichment"))
- dev.off()
- # 网络图(显示基因与GO term的关系)
- if (nrow(go_bp) >= 5) {
- pdf(file.path(vis_dir, "GO_BP_cnetplot.pdf"), width = 14, height = 10)
- print(cnetplot(go_bp, categorySize = "pvalue", foldChange = NULL,
- showCategory = 10, node_label = "all"))
- dev.off()
- }
- }
- # KEGG可视化
- if (!is.null(kegg) && nrow(kegg) > 0) {
- pdf(file.path(vis_dir, "KEGG_barplot.pdf"), width = 12, height = 8)
- print(barplot(kegg, showCategory = 20, title = "KEGG Pathway Enrichment"))
- dev.off()
- pdf(file.path(vis_dir, "KEGG_dotplot.pdf"), width = 12, height = 8)
- print(dotplot(kegg, showCategory = 20, title = "KEGG Pathway Enrichment"))
- dev.off()
- # KEGG通路图(需要安装pathview)
- tryCatch({
- if (!require("pathview", quietly = TRUE)) {
- BiocManager::install("pathview")
- }
- library(pathview)
- # 为每个显著富集的KEGG通路生成通路图
- sig_kegg_ids <- kegg@result$ID[1:min(5, nrow(kegg))]
- for (pid in sig_kegg_ids) {
- tryCatch({
- pathview(gene.data = gene_entrez$ENTREZID,
- pathway.id = pid,
- species = "hsa",
- limit = list(gene = 5, cpd = 1))
- }, error = function(e) {
- cat("无法生成通路图", pid, ":", e$message, "\n")
- })
- }
- # 移动生成的PNG文件到可视化目录
- png_files <- list.files(pattern = "hsa.*\\.png$")
- if (length(png_files) > 0) {
- file.copy(png_files, vis_dir)
- file.remove(png_files)
- }
- }, error = function(e) {
- cat("路径可视化失败:", e$message, "\n")
- })
- }
- # 4. 结果汇总
- cat("\n========== 富集分析结果汇总 ==========\n")
- cat("输入基因总数:", length(gene_list), "\n")
- cat("成功转换的基因数:", nrow(gene_entrez), "\n")
- cat("\nGO富集结果:\n")
- cat(" Biological Process:", ifelse(nrow(go_bp) > 0, paste(nrow(go_bp), "个显著条目"), "无显著条目"), "\n")
- cat(" Molecular Function:", ifelse(nrow(go_mf) > 0, paste(nrow(go_mf), "个显著条目"), "无显著条目"), "\n")
- cat(" Cellular Component:", ifelse(nrow(go_cc) > 0, paste(nrow(go_cc), "个显著条目"), "无显著条目"), "\n")
- cat("\nKEGG富集结果:\n")
- cat(" 显著通路:", ifelse(!is.null(kegg) && nrow(kegg) > 0, paste(nrow(kegg), "个"), "无"), "\n")
- cat("\n结果文件保存位置:\n")
- cat(" 数据文件:", output_dir, "\n")
- cat(" 可视化文件:", vis_dir, "\n")
- # 5. 显示部分结果
- if (nrow(go_bp) > 0) {
- cat("\nTop 10 GO Biological Process:\n")
- print(head(go_bp@result[, c("Description", "pvalue", "qvalue", "Count")], 10))
- }
- if (!is.null(kegg) && nrow(kegg) > 0) {
- cat("\nTop 10 KEGG Pathways:\n")
- print(head(kegg@result[, c("Description", "pvalue", "qvalue", "Count")], 10))
- }
- # 6. 可选:生成HTML报告
- tryCatch({
- if (!require("rmarkdown", quietly = TRUE)) {
- install.packages("rmarkdown")
- }
- # 创建简化的报告
- report_file <- file.path(output_dir, "enrichment_report.Rmd")
- report_content <- '---
- title: "基因富集分析报告"
- output: html_document
- date: "`r Sys.Date()`"
- ---
- ```{r setup, include=FALSE}
- knitr::opts_chunk$set(echo = FALSE, warning = FALSE, message = FALSE)
- library(clusterProfiler)
- library(org.Hs.eg.db)
- library(enrichplot)
- library(ggplot2)
EPI4-11-983-s003.r, no license · at the source
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The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.
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Its files are read in the Code ↔ Paper reader above, with 1 match between paragraphs and lines of code.
supp:PMC13238734/EPI4-11-983-s003.r
Availability: 1 check, the latest on 30 September 2026: the link answers (HTTP 200)
- 30 September 2026: the link answers (HTTP 200)
1 file
- EPI4-11-983-s003.r, R, 255 lines, 1 match
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Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
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- it says that the data are available on request
Read it in the paper: doi.org/10.1002/epi4.70270.
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 5 authors, 5 keywords, 13 MeSH terms, 2 funders, 45 references.
Cite
This paper
Yang, Z., Zhang, X., Guo, J., Wei, Y., & Li, J. (2026). MiR-30a-5p mediates epileptogenesis in epilepsy models by targeting SOX4 to regulate the Wnt/
BibTeX
@article{yang2026mir,
author = {Yang, Zhenlin and Zhang, Xu and Guo, Jingjing and Wei, Yuanxin and Li, Jinzi},
title = {{MiR-30a-5p mediates epileptogenesis in epilepsy models by targeting SOX4 to regulate the Wnt/
journal = {Epilepsia open},
year = {2026},
month = apr,
volume = {11},
number = {3},
pages = {983--1002},
publisher = {Wiley},
issn = {2470-9239},
doi = {10.1002/
url = {https://
pmid = {42029101},
pmcid = {PMC13238734}
}
RIS
TY - JOUR
AU - Yang, Zhenlin
AU - Zhang, Xu
AU - Guo, Jingjing
AU - Wei, Yuanxin
AU - Li, Jinzi
TI - MiR-30a-5p mediates epileptogenesis in epilepsy models by targeting SOX4 to regulate the Wnt/
T2 - Epilepsia open
J2 - Epilepsia Open
PY - 2026
DA - 2026/
VL - 11
IS - 3
SP - 983
EP - 1002
SN - 2470-9239
PB - Wiley
DO - 10.1002/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1002/
"type": "article-journal",
"title": "MiR-30a-5p mediates epileptogenesis in epilepsy models by targeting SOX4 to regulate the Wnt/
"container-title": "Epilepsia open",
"author": [
{
"family": "Yang",
"given": "Zhenlin"
},
{
"family": "Zhang",
"given": "Xu"
},
{
"family": "Guo",
"given": "Jingjing"
},
{
"family": "Wei",
"given": "Yuanxin"
},
{
"family": "Li",
"given": "Jinzi"
}
],
"container-title-short":
"volume": "11",
"issue": "3",
"page": "983-1002",
"DOI": "10.1002/
"PMID": "42029101",
"PMCID": "PMC13238734",
"ISSN": "2470-9239",
"publisher": "Wiley",
"URL": "https://
"language": "en",
"issued": {
"date-parts": [
[
2026,
4,
24
]
]
}
}
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