Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research.

Overview

Authors: Estera Jeruzalska¹, Carolin Ketteler², Elisa Stützenberger¹, Sandra Burczyk¹, Larissa Möller¹, Dierk Niessing^1,2

ORCID iDs: Dierk Niessing

Institute of Pharmaceutical Biotechnology, Ulm University, James-Franck-Ring N27, Ulm, 89081, Germany
Molecular Targets and Therapeutics Center, Institute of Structural Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr. 1, Neuherberg, 85764, Germany

Institutions: Universität Ulm (Germany); Helmholtz Munich (Germany)

Journal: Biochemistry and biophysics reports, volume 46, article 102655

Dates: received 8 January 2026; accepted 27 May 2026; published online 2 June 2026

Type: Brief report · Language: English

License: CC BY

Identifiers: DOI 10.1016/j.bbrep.2026.102655 · PMID 42282851 · PMCID PMC13251743 · OpenAlex W7163189724

Open access: gold, a free copy (OpenAlex)

Status: data only

Categories: human (organism)

Methods: Statistics, Evoked potentials

Keywords: Human neural stem cells, Immortalized cell line, Neurodegeneration, Neurodevelopment, Astrocytes, Neurons, Oligodendrocytes, hNS1

Topic: Neurogenesis and neuroplasticity mechanisms (Developmental Neuroscience, Neuroscience), according to OpenAlex

Funding: Deutsche Forschungsgemeinschaft (541627224, NI 2094/11-1)

Citations: not cited yet (Europe PMC); 37 references in the paper

Abstract

Studying neural-related questions is inherently challenging due to the limited number of suitable cell models. Here, we characterize a previously reported immortalized human neural stem cell line, HNSC.100, serving as a robust model for a wide range of neurobiological research questions. The cell line expresses key neural stem cell markers, including SOX2, vimentin, nestin, and allows for efficient genetic manipulation. Furthermore, HNSC.100 cells can be differentiated into neurons, astrocytes, and oligodendrocytes, thereby covering a wide spectrum of major neural cell types. We adapted corresponding differentiation protocols and established a comprehensive panel of molecular markers to validate successful differentiation, enabling precise characterization of the resulting cell population. In addition, we provide a complete dataset of RNA expression levels for all detectable genes in HNSC.100 cells. Based on this dataset, we assembled a list of expressed genes implicated in neural disorders that can be studied with this cell line. Together, we present a detailed characterization of the HNSC.100 cell line and provide new tools and reference data to facilitate its use. This resource enables researchers to evaluate the line's suitability for specific applications and to rapidly integrate HNSC.100 cells into their experimental workflows.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data

Datasets cited

geo:GSE317784, at NCBI GEO; found in the text, “RNA sequencing”

Data availability

Data will be made available on request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

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Version 2, 28 September 2026

Authors: added Dierk Niessing (0000-0002-5589-369X); removed Dierk Niessing

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 6 authors, 8 keywords, 1 funder, 36 references.

Cite

This paper

Jeruzalska, E., Ketteler, C., Stützenberger, E., Burczyk, S., Möller, L., & Niessing, D. (2026). Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research. Biochemistry and biophysics reports, 46, 102655. https://doi.org/10.1016/j.bbrep.2026.102655

BibTeX

@article{jeruzalska2026comprehensive, author = {Jeruzalska, Estera and Ketteler, Carolin and Stützenberger, Elisa and Burczyk, Sandra and Möller, Larissa and Niessing, Dierk}, title = {{Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research}}, journal = {Biochemistry and biophysics reports}, year = {2026}, month = jun, volume = {46}, pages = {102655}, publisher = {Elsevier}, issn = {2405-5808}, doi = {10.1016/j.bbrep.2026.102655}, url = {https://doi.org/10.1016/j.bbrep.2026.102655}, pmid = {42282851}, pmcid = {PMC13251743} }

RIS

TY - JOUR AU - Jeruzalska, Estera AU - Ketteler, Carolin AU - Stützenberger, Elisa AU - Burczyk, Sandra AU - Möller, Larissa AU - Niessing, Dierk TI - Comprehensive characterization of the human neural stem cell line HNSC.100 as a versatile model for neurobiological research T2 - Biochemistry and biophysics reports J2 - Biochem Biophys Rep PY - 2026 DA - 2026/06/02 VL - 46 SP - 102655 SN - 2405-5808 PB - Elsevier DO - 10.1016/j.bbrep.2026.102655 UR - https://doi.org/10.1016/j.bbrep.2026.102655 LA - en ER -

CSL-JSON

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