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Distinct synaptic mechanisms underlie NRXN1 variant and disorder background-dependent phenotypes in iPSC-derived neurons.

Overview

Authors: Jay English1,2, Danny McSweeney1,2, Jinghui Geng3,4, Ethan Howell1, Fumiko Ribbe1, Matthew Hinderhofer1,2, Lydia Proskauer1,2, Rebecca Sebastian1, Le Wang5,6, Tal Sharf3, Zhiping P Pang5,6, ChangHui Pak1,7
ORCID iDs: Jay English, ChangHui Pak
  1. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA
  2. Graduate Program in Molecular and Cellular Biology, University of Massachusetts Amherst, Amherst, MA 01003, USA
  3. Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
  4. Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
  5. Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA
  6. Center for NeuroMetabolism, Child Health Institute of New Jersey, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA
  7. Lead contact
Institutions: University of Massachusetts Amherst (United States); University of California, Santa Cruz (United States); Rutgers, The State University of New Jersey (United States)
Journal: Cell reports, volume 45, issue 4, article 117115
Dates: published online 20 March 2026; in print March 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.celrep.2026.117115 · PMID 41865373 · PMCID PMC13183171 · OpenAlex W7139997147
Open access: gold, a free copy (OpenAlex)
Status: code on request (how the code is found and verified)
Categories: extracellular electrophysiology (units, LFP) (modality), human (organism), autism (population), schizophrenia / psychosis (population), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics, Machine learning, Evoked potentials, Single-unit activity, calcium imaging
Keywords: Schizophrenia, Synapse, synaptic transmission, Autism Spectrum Disorders, Nrxn1, E-i Balance, Human Ipscs, Induced Neurons, Cp: Neuroscience, Cp: Stem Cell Research, Hd-Meas
Topic: Neuroscience and Neuropharmacology Research (Cellular and Molecular Neuroscience, Neuroscience), according to OpenAlex
Funding: NIMH NIH HHS (R01 MH122519); NIMH; NIGMS
Citations: not cited yet (Europe PMC); 52 references in the paper
Research resources: Rabbit anti-VGAT primary antibody RRID:AB_2301998, Goat anti-Rabbit 546 secondary antibody RRID:AB_2534077, Goat anti-mouse 647 secondary antibody RRID:AB_2535813, Mouse anti VGLUT1 primary Antibody RRID:AB_887877, pMDLg/pRRE RRID:Addgene_12251, pRSV-Rev RRID:Addgene_12253, pMD2.G (aka VSVG) RRID:Addgene_12259, pTetO-Ascl1-T2A-PuroR RRID:Addgene_162345, Tet-O-FUW-EGFP RRID:Addgene_30130, pTet-O-Ngn2-Puro RRID:Addgene_52047, pLV-TetO-mCherry RRID:Addgene_70273, pTet-O-FUW-Dlx2-hygroR RRID:Addgene_97330, HEK293T human cells RRID:CVCL_0063, MATLAB R2024a RRID:SCR_001622, Prism 10.5.0 RRID:SCR_002798, Imaris Software RRID:SCR_007370, RRID:SCR_011323, Office Suite RRID:SCR_016137, NIS Elements-Advanced Research RRID:SCR_027181

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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Data

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Code and data availability statement

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Read it in the paper: doi.org/10.1016/j.celrep.2026.117115.

Versions

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Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 12 authors, 11 keywords, 3 funders, 51 references, 19 RRIDs.

Cite

This paper

English, J., McSweeney, D., Geng, J., Howell, E., Ribbe, F., Hinderhofer, M., Proskauer, L., Sebastian, R., Wang, L., Sharf, T., Pang, Z. P., & Pak, C. (2026). Distinct synaptic mechanisms underlie NRXN1 variant and disorder background-dependent phenotypes in iPSC-derived neurons. Cell reports, 45(4), 117115. https://doi.org/10.1016/j.celrep.2026.117115

BibTeX

@article{english2026distinct,
author = {English, Jay and McSweeney, Danny and Geng, Jinghui and Howell, Ethan and Ribbe, Fumiko and Hinderhofer, Matthew and Proskauer, Lydia and Sebastian, Rebecca and Wang, Le and Sharf, Tal and Pang, Zhiping P and Pak, ChangHui},
title = {{Distinct synaptic mechanisms underlie NRXN1 variant and disorder background-dependent phenotypes in iPSC-derived neurons}},
journal = {Cell reports},
year = {2026},
month = mar,
volume = {45},
number = {4},
pages = {117115},
publisher = {Cell Press},
issn = {2211-1247},
doi = {10.1016/j.celrep.2026.117115},
url = {https://doi.org/10.1016/j.celrep.2026.117115},
pmid = {41865373},
pmcid = {PMC13183171}
}

RIS

TY - JOUR
AU - English, Jay
AU - McSweeney, Danny
AU - Geng, Jinghui
AU - Howell, Ethan
AU - Ribbe, Fumiko
AU - Hinderhofer, Matthew
AU - Proskauer, Lydia
AU - Sebastian, Rebecca
AU - Wang, Le
AU - Sharf, Tal
AU - Pang, Zhiping P
AU - Pak, ChangHui
TI - Distinct synaptic mechanisms underlie NRXN1 variant and disorder background-dependent phenotypes in iPSC-derived neurons
T2 - Cell reports
J2 - Cell Rep
PY - 2026
DA - 2026/03/20
VL - 45
IS - 4
SP - 117115
SN - 2211-1247
PB - Cell Press
DO - 10.1016/j.celrep.2026.117115
UR - https://doi.org/10.1016/j.celrep.2026.117115
LA - en
ER -

CSL-JSON

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