OSCR

GLIS3 marks a neural-like progenitor cell state that drives metastasis in pancreatic ductal adenocarcinoma.

Overview

Authors: Dennis Gong1,2,3,4, Jimmy A Guo2,5,6,4, Jennifer Su2,7, Sueda Cetinkaya6,7, Connor Hennessey6, Patrick Yu2, Ananya Jambhale6,8, Peter L Wang1,2, Nicholas J Caldwell9, Ashley Lam1,2,10, Junning Wang6, Carina Shiau1,2,11, Kevin S Kapner6, Julien Dilly6, Laleh Abbassi6, Seema Chugh6, Shatavisha Dasgupta12, Jonathan Nowak13, Brian M Wolpin6, M Lisa Zhang9, Mari Mino-Kenudson9, Tyler Jacks14, Andrew J Aguirre2,6,15, William L Hwang1,2,15,16
ORCID iDs: Jennifer Su, Ashley Lam, Andrew J Aguirre, William L Hwang
16 affiliations
  1. Center for Systems Biology, Krantz Family Center for Cancer Research, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  2. Broad Institute of MIT and Harvard, Cambridge, MA, USA
  3. Program in Health Sciences and Technology, Massachusetts Institute of Technology and Harvard University, Cambridge, MA, USA
  4. These authors contributed equally
  5. School of Medicine, University of California, San Francisco, San Francisco, CA, USA
  6. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA
  7. Harvard University, Graduate School of Arts and Sciences, Cambridge, MA, USA
  8. Grossman School of Medicine, New York University, New York, NY, USA
  9. Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  10. Department of Surgery and Interventional Science Centre for 3D Models of Health and Disease, and Department of Cell and Developmental Biology Consortium for Mitochondrial Research, University College London, London, UK
  11. Harvard Medical School, Boston, MA, USA
  12. Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA
  13. Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA
  14. Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
  15. Senior author
  16. Lead contact
Institutions: Broad Institute (United States); Harvard University (United States); Massachusetts General Hospital (United States); Center for Systems Biology (United States); Massachusetts Institute of Technology (United States); University of California, San Francisco (United States); Dana-Farber Cancer Institute (United States); Brigham and Women's Hospital (United States); New York University (United States); University College London (United Kingdom); Koch Institute for Integrative Cancer Research At MIT (United States); Dana-Farber/Harvard Cancer Center (United States)
Journal: Cell reports, volume 45, issue 5, article 117314
Dates: published online 27 April 2026; in print 26 May 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.celrep.2026.117314 · PMID 42048185 · PMCID PMC13479318 · OpenAlex W4414532433
Open access: gold, a free copy (OpenAlex)
Preprint: doi:10.1101/2025.09.18.677140
Status: code on request
Categories: human (organism), mouse (organism), other condition (population), cellular / molecular (subfield)
Methods: Statistics
Keywords: Pancreatic cancer, Chemoresistance, Residual Disease, Glis3, Cell States, Cp: Cancer, Neural-like Progenitor
MeSH: Carcinoma, Pancreatic Ductal*, Neoplastic Stem Cells*, Neural Stem Cells*, Pancreatic Neoplasms*, Transcription Factors*, Animals, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Mice, Neoplasm Metastasis (* major topic)
Topic: Pancreatic and Hepatic Oncology Research (Oncology, Medicine), according to OpenAlex
Funding: Pancreatic Cancer Action Network Inc; Burroughs Wellcome Fund; National Science Foundation; NCI NIH HHS (K08 CA270417); National Institutes of Health National Cancer Institute (K08CA270417)
Citations: not cited yet (Europe PMC); 65 references in the paper
Research resources: CD44 RRID:AB_1107649, AF488 conjugated Pan-Cytokeratin RRID:AB_1834350, Phalloidin RRID:AB_2315633, AF647 Anti-Rabbit Secondary Antibody RRID:AB_2535812, Gata6 RRID:AB_2539879, Glis3 RRID:AB_3285315, Keratin 17 RRID:AB_559766, psPAX2 RRID:Addgene_12260, lentiGuide-Puro RRID:Addgene_52963, pMD2.G (VSV-G) RRID:Addgene_8454, HEK293T RRID:CVCL_0063, AsPC-1 RRID:CVCL_0152, BxPC-3 RRID:CVCL_0186, MIAPaCa-2 RRID:CVCL_0428, PANC-1 RRID:CVCL_0480, CFPAC-1 RRID:CVCL_1119, HUPT4 RRID:CVCL_1300, KP4 RRID:CVCL_1338, Panc0203 RRID:CVCL_1633, Panc0403 RRID:CVCL_1636, PSN1 RRID:CVCL_1644, SW1990 RRID:CVCL_1723, Patu8988S RRID:CVCL_1846, Patu8988T RRID:CVCL_1847, PaCaDD119 RRID:CVCL_1848, SUIT-2 RRID:CVCL_3172, HPAC RRID:CVCL_3517, DESeq2 RRID:SCR_000154, ImageJ RRID:SCR_003070, CellProfiler RRID:SCR_007358, IncuCyte S3 Live Cell Analysis System RRID:SCR_023147, CRISPick RRID:SCR_025148

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none (its code is available on request), so it has no map.

Data

Datasets cited

Code and data availability statement

The paper has a code and data availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:

Read it in the paper: doi.org/10.1016/j.celrep.2026.117314.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 30 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 24 authors, 7 keywords, 11 MeSH terms, 5 funders, 64 references, 32 RRIDs.

Cite

This paper

Gong, D., Guo, J. A., Su, J., Cetinkaya, S., Hennessey, C., Yu, P., Jambhale, A., Wang, P. L., Caldwell, N. J., Lam, A., Wang, J., Shiau, C., Kapner, K. S., Dilly, J., Abbassi, L., Chugh, S., Dasgupta, S., Nowak, J., Wolpin, B. M., . . . Hwang, W. L. (2026). GLIS3 marks a neural-like progenitor cell state that drives metastasis in pancreatic ductal adenocarcinoma. Cell reports, 45(5), 117314. https://doi.org/10.1016/j.celrep.2026.117314

BibTeX

@article{gong2026glis3,
author = {Gong, Dennis and Guo, Jimmy A and Su, Jennifer and Cetinkaya, Sueda and Hennessey, Connor and Yu, Patrick and Jambhale, Ananya and Wang, Peter L and Caldwell, Nicholas J and Lam, Ashley and Wang, Junning and Shiau, Carina and Kapner, Kevin S and Dilly, Julien and Abbassi, Laleh and Chugh, Seema and Dasgupta, Shatavisha and Nowak, Jonathan and Wolpin, Brian M and Zhang, M Lisa and Mino-Kenudson, Mari and Jacks, Tyler and Aguirre, Andrew J and Hwang, William L},
title = {{GLIS3 marks a neural-like progenitor cell state that drives metastasis in pancreatic ductal adenocarcinoma}},
journal = {Cell reports},
year = {2026},
month = apr,
volume = {45},
number = {5},
pages = {117314},
publisher = {Cell Press},
issn = {2211-1247},
doi = {10.1016/j.celrep.2026.117314},
url = {https://doi.org/10.1016/j.celrep.2026.117314},
pmid = {42048185},
pmcid = {PMC13479318}
}

RIS

TY - JOUR
AU - Gong, Dennis
AU - Guo, Jimmy A
AU - Su, Jennifer
AU - Cetinkaya, Sueda
AU - Hennessey, Connor
AU - Yu, Patrick
AU - Jambhale, Ananya
AU - Wang, Peter L
AU - Caldwell, Nicholas J
AU - Lam, Ashley
AU - Wang, Junning
AU - Shiau, Carina
AU - Kapner, Kevin S
AU - Dilly, Julien
AU - Abbassi, Laleh
AU - Chugh, Seema
AU - Dasgupta, Shatavisha
AU - Nowak, Jonathan
AU - Wolpin, Brian M
AU - Zhang, M Lisa
AU - Mino-Kenudson, Mari
AU - Jacks, Tyler
AU - Aguirre, Andrew J
AU - Hwang, William L
TI - GLIS3 marks a neural-like progenitor cell state that drives metastasis in pancreatic ductal adenocarcinoma
T2 - Cell reports
J2 - Cell Rep
PY - 2026
DA - 2026/04/27
VL - 45
IS - 5
SP - 117314
SN - 2211-1247
PB - Cell Press
DO - 10.1016/j.celrep.2026.117314
UR - https://doi.org/10.1016/j.celrep.2026.117314
LA - en
ER -

CSL-JSON

{
"id": "10.1016/j.celrep.2026.117314",
"type": "article-journal",
"title": "GLIS3 marks a neural-like progenitor cell state that drives metastasis in pancreatic ductal adenocarcinoma",
"container-title": "Cell reports",
"author": [
{
"family": "Gong",
"given": "Dennis"
},
{
"family": "Guo",
"given": "Jimmy A"
},
{
"family": "Su",
"given": "Jennifer"
},
{
"family": "Cetinkaya",
"given": "Sueda"
},
{
"family": "Hennessey",
"given": "Connor"
},
{
"family": "Yu",
"given": "Patrick"
},
{
"family": "Jambhale",
"given": "Ananya"
},
{
"family": "Wang",
"given": "Peter L"
},
{
"family": "Caldwell",
"given": "Nicholas J"
},
{
"family": "Lam",
"given": "Ashley"
},
{
"family": "Wang",
"given": "Junning"
},
{
"family": "Shiau",
"given": "Carina"
},
{
"family": "Kapner",
"given": "Kevin S"
},
{
"family": "Dilly",
"given": "Julien"
},
{
"family": "Abbassi",
"given": "Laleh"
},
{
"family": "Chugh",
"given": "Seema"
},
{
"family": "Dasgupta",
"given": "Shatavisha"
},
{
"family": "Nowak",
"given": "Jonathan"
},
{
"family": "Wolpin",
"given": "Brian M"
},
{
"family": "Zhang",
"given": "M Lisa"
},
{
"family": "Mino-Kenudson",
"given": "Mari"
},
{
"family": "Jacks",
"given": "Tyler"
},
{
"family": "Aguirre",
"given": "Andrew J"
},
{
"family": "Hwang",
"given": "William L"
}
],
"container-title-short": "Cell Rep",
"volume": "45",
"issue": "5",
"page": "117314",
"DOI": "10.1016/j.celrep.2026.117314",
"PMID": "42048185",
"PMCID": "PMC13479318",
"ISSN": "2211-1247",
"publisher": "Cell Press",
"URL": "https://doi.org/10.1016/j.celrep.2026.117314",
"language": "en",
"issued": {
"date-parts": [
[
2026,
4,
27
]
]
}
}

Similar papers

The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.

[1] doi:10.1186/s12967-026-08447-w
Single-cell and spatial transcriptomes reveal oligodendrocyte remodeling fatty acid metabolism microenvironment of lung cancer brain metastases.
Journal: Journal of translational medicine
In common: other condition, cellular / molecular, 2 references
[2] doi:10.1172/jci195639
Therapeutic delivery of microRNAs discovered to target deregulated glioblastoma pathways inhibits tumor growth in mice.
Journal: The Journal of clinical investigation
In common: other condition, mouse, cellular / molecular, 1 reference
[3] doi:10.1038/s41467-026-72932-5
Tumor-derived GDF15 induces CCN3⁺ Schwann cells to promote cancer pain in pancreatic cancer.
Journal: Nature communications
In common: other condition, mouse, cellular / molecular, 1 reference
[4] doi:10.1038/s41467-026-72927-2
Complex I protein NDUFB9 is a metabolic vulnerability in triple negative breast cancer brain metastases.
Journal: Nature communications
In common: other condition, mouse, cellular / molecular, 1 reference
[5] doi:10.1038/s41598-026-51848-6
SPARC is associated with tumor nerve stroma interactions and perineural invasion in pancreatic ductal adenocarcinoma.
Journal: Scientific reports
In common: other condition, mouse, 1 reference
[6] doi:10.1038/s41592-026-03057-2 [code]
CREsted: modeling genomic and synthetic cell-type-specific enhancers across tissues and species.
Journal: Nature methods
In common: mouse, 1 reference
[7] doi:10.1038/s41467-026-71112-9 [code]
Modelling synaptic dysfunction in childhood dementia using human iPSC-derived cortical networks.
Journal: Nature communications
In common: cellular / molecular, 1 reference
[8] doi:10.1371/journal.pcbi.1014573 [code]
Cell-type-specific m1A dynamics are associated with microglial phenotypic transition and neuronal metabolic adaptation during spinal cord injury.
Journal: PLoS computational biology
In common: other condition, mouse, cellular / molecular, 1 reference
[9] doi:10.1016/j.omta.2026.201835
Base editing rescues a hereditary motor neuron disease in mouse and patient-derived iPSC organoid models.
Journal: Molecular therapy. Advances
In common: other condition, mouse, 1 reference
[10] doi:10.1007/s11060-026-05515-5
Human neural organoid modeling of diffuse midline glioma captures the complexity of patient tumors.
Journal: Journal of neuro-oncology
In common: other condition, mouse, 1 reference

Contribute

The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.

Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.

Request its removal

To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).

Discussion, reproductions, activity

Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.

Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.

Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.