Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease.
Overview
- Institute of Reconstructive Neurobiology, University of Bonn Medical Faculty & University Hospital Bonn, 53127 Bonn, Germany
- Genetics and Aging Research Unit, MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, McCance Center for Brain Health, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
- German Center for Neurodegenerative Diseases (DZNE), 53127 Bonn, Germany
- Cellomics Department, LIFE & BRAIN GmbH, 53127 Bonn, Germany
- Pharma Research and Early Development, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland
- Cell Programming Core Facility, University of Bonn, 53127 Bonn, Germany
Abstract
This study aims at implementing a 3D cell culture model of Alzheimer’s disease (AD). To that end we engineered human induced pluripotent stem cell (iPSC)-derived neural stem cells to conditionally overexpress FAD mutant APP and PSEN1 variants. After differentiation in 3D basement membrane matrices, cultures exhibited increased Aβ42 and Aβ40 levels and a highly pathogenic shift of the Aβ42/
Reproduced under the paper's license (CC BY), from the paper cited above.
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All data reported in this paper will be shared by the lead contact upon request.
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Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
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Versions
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 6 keywords, 13 MeSH terms, 7 funders, 75 references, 11 RRIDs.
Cite
This paper
Hebisch, M., Kamin, V., Cenini, G., Piazzesi, A., Bertan, F., Weykopf, B., Schlee, J., Kaniyappan, S., Washicosky, K. J., Kim, D. Y., Bano, D., Peitz, M., & Brüstle, O. (2026). Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease. Cell reports methods, 6(4), 101365. https://
BibTeX
@article{hebisch2026reca
author = {Hebisch, Matthias and Kamin, Viola and Cenini, Giovanna and Piazzesi, Antonia and Bertan, Fabio and Weykopf, Beatrice and Schlee, Julia and Kaniyappan, Senthilvelrajan and Washicosky, Kevin J and Kim, Doo Yeon and Bano, Daniele and Peitz, Michael and Brüstle, Oliver},
title = {{Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease}},
journal = {Cell reports methods},
year = {2026},
month = mar,
volume = {6},
number = {4},
pages = {101365},
publisher = {Elsevier},
issn = {2667-2375},
doi = {10.1016/
url = {https://
pmid = {41916306},
pmcid = {PMC13106978}
}
RIS
TY - JOUR
AU - Hebisch, Matthias
AU - Kamin, Viola
AU - Cenini, Giovanna
AU - Piazzesi, Antonia
AU - Bertan, Fabio
AU - Weykopf, Beatrice
AU - Schlee, Julia
AU - Kaniyappan, Senthilvelrajan
AU - Washicosky, Kevin J
AU - Kim, Doo Yeon
AU - Bano, Daniele
AU - Peitz, Michael
AU - Brüstle, Oliver
TI - Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease
T2 - Cell reports methods
J2 - Cell Rep Methods
PY - 2026
DA - 2026/
VL - 6
IS - 4
SP - 101365
SN - 2667-2375
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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