Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke.

Overview

1. Department of Pathology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
2. Clinical Skills Training Center, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
3. Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
4. Institute of Basic Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, China
5. Department of Neurosurgery, Chongqing General Hospital, Chongqing University, Chongqing 400016, China
6. College of Pharmacy, Chongqing Medical University, Chongqing 400016, China

Abstract

Ischemic stroke (IS) imposes a major global health burden. To uncover new diagnostic and therapeutic targets, we profiled neuronal heterogeneity during IS using single-cell RNA sequencing. Our analysis decoded neuronal lineage trajectories, identified a critical cell-cell communication network, and pinpointed key gene modules. By integrating multiple machine learning algorithms, we constructed a highly accurate diagnostic model based on the hub genes Il18 and Cherp. This model was rigorously validated across multi-omics datasets and species. We further confirmed that Cherp promotes neuronal repair by regulating calcium homeostasis, while Il18 serves as a potential early blood biomarker. This work provides effective targets and translatable tools for advancing IS precision medicine.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Code

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Data

Datasets cited

• geo:GSE174574, at NCBI GEO; found in “Data and code availability”

Data and code availability

This paper analyzes existing, publicly available data, accessible at GSE174574 (https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE174574), GSE58294 (https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58294), GSE61616 (https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE61616), and GSE16561 (https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE16561).

This paper does not report original code.

Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY-NC), from the paper cited above.

Versions

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 8 authors, 3 keywords, 50 references, 4 RRIDs.

Cite

This paper

Wang, X., Xu, Q., Wang, X., Li, L., Wang, L., Shao, C., Yang, X., & Wang, X. (2026). Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke. iScience, 29(6), 116257. https://doi.org/10.1016/j.isci.2026.116257

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