Lipid nanoparticles enable mRNA delivery to diverse cell types of the inner Retina.
Overview
- Retinal Physiology and Gene Therapy Lab, Department of Neurophysiology, Institute of Physiology and Pathophysiology, University of Marburg, 35037 Marburg, Germany
- Department of Neurophysiology, Institute of Physiology and Pathophysiology, University of Marburg, 35037 Marburg, Germany
- The Nuffield Laboratory of Ophthalmology, Sleep and Circadian Neuroscience Institute, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX1 3QU, UK
- Department of Ophthalmology, University Hospitals of Giessen and Marburg, Marburg Campus, Marburg, Germany
Abstract
Lipid nanoparticles (LNPs) have emerged as a promising platform for retinal genetic therapy, offering a non-viral alternative to adeno-associated viruses. Although LNPs can transfect outer retinal cells, their transfection profile across inner retinal cell types remains insufficiently characterized. Here, we systematically assessed the cell-type transfection profile of conventional LNPs encapsulating chemically modified mRNA encoding mCherry in murine retinal explants, complemented by experiments in dissociated retinal cell cultures. We compared quasi-subretinal and quasi-intravitreal administrations and evaluated how retinal degeneration and inner limiting membrane (ILM) integrity influence LNP-mediated transfections. We observed that LNPs efficiently transfected Müller glia under all experimental conditions. In addition, LNPs transfected several other retinal cell types, including neurons in dissociated cells and explants, and vascular cells exclusively in explants. Subretinal delivery resulted in higher transfection rates than intravitreal administration, and overall efficiency was higher in degenerate as compared to non-degenerate healthy retinas. In healthy retinas, removal of ILM increased transfection efficiency following intravitreal administration. Together, these findings demonstrate that conventional LNPs can transfect a broader range of retinal cell types than previously recognized and highlight LNPs as a tool for mRNA delivery to the retina, with applications in gene supplementation, editing, and regenerative therapies for inner retinal disorders.
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.
The paper's code and data availability statement is in the Data section.
Tracing map
A tracing map links a paper to the code its authors published: this paper has none (its code is available on request), so it has no map.
Data
No dataset and no data link were found in the paper.
Data and code availability
The datasets generated during and/
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 2, 28 September 2026
- Authors: added Eleonora Carpentiero (0009-0001-8513-4598); Paula Streckenbach (0009-0005-5365-6032); Vijay Renigunta (0000-0003-4123-4447); removed Eleonora Carpentiero; Paula Streckenbach; Vijay Renigunta
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 11 keywords, 2 funders, 58 references, 5 RRIDs.
Cite
This paper
Biswas, S., Carpentiero, E., Xhaferri, N., Streckenbach, P., Renigunta, V., & Lindner, M. (2026). Lipid nanoparticles enable mRNA delivery to diverse cell types of the inner Retina. Molecular therapy. Nucleic acids, 37(3), 102996. https://
BibTeX
@article{biswas2026lipid
author = {Biswas, Sumit and Carpentiero, Eleonora and Xhaferri, Nermina and Streckenbach, Paula and Renigunta, Vijay and Lindner, Moritz},
title = {{Lipid nanoparticles enable mRNA delivery to diverse cell types of the inner Retina}},
journal = {Molecular therapy. Nucleic acids},
year = {2026},
month = jun,
volume = {37},
number = {3},
pages = {102996},
publisher = {American Society of Gene \& Cell Therapy},
issn = {2162-2531},
doi = {10.1016/
url = {https://
pmid = {42519573},
pmcid = {PMC13382181}
}
RIS
TY - JOUR
AU - Biswas, Sumit
AU - Carpentiero, Eleonora
AU - Xhaferri, Nermina
AU - Streckenbach, Paula
AU - Renigunta, Vijay
AU - Lindner, Moritz
TI - Lipid nanoparticles enable mRNA delivery to diverse cell types of the inner Retina
T2 - Molecular therapy. Nucleic acids
J2 - Mol Ther Nucleic Acids
PY - 2026
DA - 2026/
VL - 37
IS - 3
SP - 102996
SN - 2162-2531
PB - American Society of Gene & Cell Therapy
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1016/
"type": "article-journal",
"title": "Lipid nanoparticles enable mRNA delivery to diverse cell types of the inner Retina",
"container-title": "Molecular therapy. Nucleic acids",
"author": [
{
"family": "Biswas",
"given": "Sumit"
},
{
"family": "Carpentiero",
"given": "Eleonora"
},
{
"family": "Xhaferri",
"given": "Nermina"
},
{
"family": "Streckenbach",
"given": "Paula"
},
{
"family": "Renigunta",
"given": "Vijay"
},
{
"family": "Lindner",
"given": "Moritz"
}
],
"container-title-short":
"volume": "37",
"issue": "3",
"page": "102996",
"DOI": "10.1016/
"PMID": "42519573",
"PMCID": "PMC13382181",
"ISSN": "2162-2531",
"publisher": "American Society of Gene & Cell Therapy",
"URL": "https://
"language": "en",
"issued": {
"date-parts": [
[
2026,
6,
30
]
]
}
}
Similar papers
The papers with a page that share the most with this one: the tools found in their code, their categories, datasets, cited references and authors, the rarest counting most.
- [1] doi:10.1016/j.omta.2026.201729
- Swine reporter model for preclinical evaluation and characterization of gene delivery vectors.Journal: Molecular therapy. AdvancesIn common: cellular / molecular, 2 references
- [2] doi:10.7554/elife.100880 [code]
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.Journal: eLifeIn common: 2 references
- [3] doi:10.1038/s41467-026-72845-3 [code]
- The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics.Journal: Nature communicationsIn common: cellular / molecular, 2 references
- [4] doi:10.1126/sciadv.aeb3209 [code]
- The actomyosin cortex controls t-tubule remodeling in skeletal muscle.Journal: Science advancesIn common: cellular / molecular, 2 references
- [5] doi:10.1016/j.isci.2026.117010 [code]
- Deep learning-assisted mapping of dendritic spines using sequential 2D two-photon calcium imaging.Journal: iScienceIn common: cellular / molecular, 2 references
- [6] doi:10.1242/jcs.264565 [code]
- A conserved Arf-GEF modulates axonal integrity through RAB-35 by altering neuron-epidermal attachment.Journal: Journal of cell scienceIn common: cellular / molecular, 2 references
- [7] doi:10.1126/sciadv.aec4911 [code]
- Dendritic shaft constrictions shape synaptic integration in neurons.Journal: Science advancesIn common: cellular / molecular, 2 references
- [8] doi:10.1126/sciadv.aeg3223 [code]
- The extreme diversity of retinal amacrine cells has deep evolutionary roots.Journal: Science advancesIn common: cellular / molecular, 2 references
- [9] doi:10.1038/s41592-026-03066-1 [code]
- A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.Journal: Nature methodsIn common: 2 references
- [10] doi:10.64898/2026.03.30.715222 [code]
- Synthetic lumen rounding directs neural progenitor division modeJournal: bioRxiv (preprint)In common: 2 references
Contribute
The authors of this paper can claim it, correct its record and validate its tracing map, and the maintainers of its code (its owner, or a public member of its organization) correct what it says of their repository; anyone signed in can ask for its removal. Every request goes to OSCR's own machine, which answers it; your account page follows them.
Sign in with ORCID to claim this paper as one of its authors, correct its record or validate its tracing map: when the paper's metadata lists your ORCID iD, you are recognized at once. Maintainers of its code: sign in with GitHub, then claim the repository on your account page.
Claim this paper
Correct its record
Say what each link of this record is, remove the ones that are not the paper's, add the ones that are missing. The correction becomes a new version of the record, in its Versions section.
Request its removal
To ask OSCR to remove this record, the copies of its authors' scripts or its tracing map, use the removal request page: signed in, you say who you are, what to remove and why, then review and confirm the request. Published rules decide every request (how).
Discussion, reproductions, activity
Discussion: questions and error reports about this paper and its code, from signed-in readers and its authors. It opens with sign-in.
Reproductions: reports from readers who ran the authors' code: what they reproduced, with which environment, commit and data. It opens with sign-in.
Activity: what happens around this paper: new versions of its record, its map's validation, discussions and reproductions. It opens with sign-in.