Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain.
Overview
- Laboratory of Neuropsychology, National Institute of Mental Health, NIH, Bethesda, MD 20892, USA
- Neuroscience Institute, Carnegie Mellon University, Pittsburgh, PA 15213, USA
- Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA
- Lipschultz Center for Cognitive Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
- School of Medicine, Wayne State University, Detroit, MI 48201, USA
- Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
- Section on Instrumentation, National Institute of Mental Health, NIH, Bethesda, MD 20892, USA
- Veterinary Medicine and Resources Branch, National Institute of Mental Health, NIH, Bethesda, MD 20892, USA
- Newcastle University, NE1 7RU Newcastle upon Tyne, UK
Abstract
Viral injections in nonhuman primates allow modification of neurons to investigate the organization and function of neural circuits, their developmental processes, and disease-related mechanisms. Here, we present a protocol for performing precise stereotaxic microinjections in the macaque brain. We describe steps for pre-surgical imaging, target planning, viral injections, and perioperative care. We then detail procedures for tissue extraction and slice electrophysiology experiments in multiple subcortical and cortical regions.
For complete details on the use and execution of this protocol, please refer to Furlanis et al.1
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
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Data and code availability
• All data reported in this paper will be shared by the lead contact upon request. • All code used in this paper will be shared by the lead contact upon request. • Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 13 authors, 3 keywords, 6 MeSH terms, 1 funder, 15 references, 1 RRID.
Cite
This paper
Tangen, A. C., Harmon, P., Plotnikova, A. S., Mohanty, A., Cummins, A. C., Pelkey, K. A., Cameron, K., Rood, C., Guerriero, K. A., McBain, C. J., Eldridge, M. A., Averbeck, B. B., & Azadi, R. (2026). Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain. STAR protocols, 7(2), 104522. https://
BibTeX
@article{tangen2026surgi
author = {Tangen, Anne Claire and Harmon, Peyton and Plotnikova, Anya S. and Mohanty, Arya and Cummins, Alexander C. and Pelkey, Kenneth A. and Cameron, Katherine and Rood, Camille and Guerriero, Kathryn Ann and McBain, Chris J. and Eldridge, Mark A.G. and Averbeck, Bruno B. and Azadi, Reza},
title = {{Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain}},
journal = {STAR protocols},
year = {2026},
month = apr,
volume = {7},
number = {2},
pages = {104522},
publisher = {Elsevier},
issn = {2666-1667},
doi = {10.1016/
url = {https://
pmid = {42054214},
pmcid = {PMC13136701}
}
RIS
TY - JOUR
AU - Tangen, Anne Claire
AU - Harmon, Peyton
AU - Plotnikova, Anya S.
AU - Mohanty, Arya
AU - Cummins, Alexander C.
AU - Pelkey, Kenneth A.
AU - Cameron, Katherine
AU - Rood, Camille
AU - Guerriero, Kathryn Ann
AU - McBain, Chris J.
AU - Eldridge, Mark A.G.
AU - Averbeck, Bruno B.
AU - Azadi, Reza
TI - Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain
T2 - STAR protocols
J2 - STAR Protoc
PY - 2026
DA - 2026/
VL - 7
IS - 2
SP - 104522
SN - 2666-1667
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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