Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity.
The 17 matches
- [1] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Interneurons_Fig2D,2E.Rmd, lines 206–218 · score 0.86 · slc6a4, slc6a2, slc6a3, pkd1l2, GABAergic, foxa2
- [2] § Results › Emergence of Xenopus transient regeneration-specific neurons at 1 dpa ↔ Fig6A,6B,6C,6E,6F, S7, S10.Rmd, lines 553–558 · score 0.85 · semaphorin plexin signaling, enriched GO term, nervous system development, cell adhesion, axon guidance, pathway
- [3] § Results › Emergence of Xenopus transient regeneration-specific neurons at 1 dpa ↔ Fig6A,6B,6C,6E,6F, S7, S10.Rmd, lines 276–324 · score 0.70 · ppp1r17, rtn4rl1, Neural Progenitor Cells, sfrp4, gap43, unknown
- [4] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Figure_S8A-E.Rmd, lines 27–102 · score 0.67 · slc6a2, slc6a3, catecholaminergic, serotonergic, tph2, gad2
- [5] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Neuron_neurotransmitters.Rmd, lines 24–56 · score 0.67 · Kolmer Agduhr interneurons, Rohon Beard neurons, en1, neurotransmitter, neural cell, vsx2
- [6] § Methods › scRNA-sequencing processing ↔ FigS8_F-K.Rmd, lines 37–72 · score 0.66 · CellChat, neural subset, ortholog, interactions, db, mouse
- [7] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Fig2A,2B,2C,3A,S3A.Rmd, lines 75–123 · score 0.64 · Kolmer Agduhr interneurons, Rohon Beard neurons, neural cell, excitatory, vsx2, inhibitory
- [8] § Results › scRNA-seq shows dynamic changes in cell type abundance over time ↔ Seurat_tail_annotations_final.Rmd, lines 132–193 · score 0.63 · basal cells, goblet cells, immune cells, syndetome, sclerotome, tail
- [9] § Results › scRNA-seq shows dynamic changes in cell type abundance over time ↔ Fig1B,1C,1D,S2,S3.Rmd, lines 23–58 · score 0.62 · basal cells, goblet cells, immune cells, syndetome, sclerotome, tail
- [10] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Interneurons_Fig2D,2E.Rmd, lines 175–204 · score 0.62 · glycinergic interneuron, KA interneuron, intermediate KA, UMAP, V2b, V1
- [11] § Results › An expanded atlas of Xenopus neuron types based on conserved markers ↔ Fig2A,2B,2C,3A,S3A.Rmd, lines 75–123 · score 0.62 · Excitatory interneurons, inhibitory interneurons, glycinergic interneuron, UMAP, Figure 2, cluster
- [12] § Methods › scRNA-sequencing processing ↔ Seurat_tail_cell_cycle_scoring_final.Rmd, lines 19–37 · score 0.61 · Cell Cycle Scoring, Seurat, gene
- [13] § Methods › scRNA-sequencing pipeline ↔ Seurat_tail_QC_final.Rmd, lines 84–184 · score 0.60 · processed Seurat, scRNA, singlets, seq
- [14] § Results › scRNA-seq shows dynamic changes in cell type abundance over time ↔ Fig1B,1C,1D,S2,S3.Rmd, lines 23–58 · score 0.59 · basal cells, goblet cells, immune cells, syndetome, sclerotome, Figure 1
- [15] § Methods › scRNA-sequencing processing ↔ Seurat_tail_QC_final.Rmd, lines 43–82 · score 0.57 · nFeature_RNA, cutoff, mitochondrial, CellRanger, UMI, Seurat
- [16] § Results › Emergence of Xenopus transient regeneration-specific neurons at 1 dpa ↔ FigS8_F-K.Rmd, lines 127–200 · score 0.56 · L1CAM, NCAM, NRG, NRXN, SEMA3, lep
- [17] § Results › Proliferative neurogenesis drives neuron repopulation from 3 to 7 dpa ↔ Seurat_tail_cell_cycle_scoring_final.Rmd, lines 19–37 · score 0.53 · cell cycle, Seurat, G2M, score, phase, genes
Paper
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The authors' code
R Markdown · 218 lines · 9.8 KB · no license · 2 matches
- ---
- title: "Motor_Neurons"
- output: html_document
- date: "2025-08-21"
- ---
- ```{r setup, include=FALSE}
- knitr::opts_chunk$set(echo = TRUE)
- ```
- ```{r}
- library(ggplot2)
- library(dplyr)
- library(Seurat)
- library(scCustomize)
- ```
- Subset dataset and run processing on Interneurons
- ```{r}
- neural <- LoadSeuratRds(file = "neural_final.rds")
- INs <- subset(x = neural, subset = ([email hidden]$neural_cell_types == "Glutamatergic Interneurons" |
- [email hidden]$neural_cell_types == "GABA/Glycinergic Interneurons" |
- [email hidden]$neural_cell_types == "Kolmer-Agduhr Interneurons" |
- [email hidden]$neural_cell_types == "vsx2+ Interneurons" |
- [email hidden]$neural_cell_types == "Differentiating Interneurons"))
- # Normalize, etc REGRESS OUT SAMPLE AND NFEAT_RNA
- INs <- NormalizeData(INs)
- INs <- ScaleData(INs, vars.to.regress = c("Sample", "nFeature_RNA"))
- INs <- FindVariableFeatures(INs, selection.method = "vst", nfeatures = 2000)
- INs <- RunPCA(INs, features = VariableFeatures(object = INs))
- INs <- FindNeighbors(INs, dims=1:50, reduction = "integrated.rpca")
- INs <- FindClusters(INs, resolution = 4, reduction.type = "integrated.rpca")
- INs <- RunUMAP(INs, dims = 1:50, reduction = "integrated.rpca")
- # Look at basic parameters
- DimPlot(INs, reduction = "umap", group.by = "neural_cell_types")
- DimPlot(INs, reduction = "umap", group.by = "neural_cell_types", split.by = "Condition")
- DimPlot(INs, reduction = "umap", group.by = "seurat_clusters", label = TRUE)
- # Re load
- INs <- LoadSeuratRds(file = "INs.rds")
- #neural <- LoadSeuratRds(file = "neural_final.rds")
- ```
- Looking at some markers to identify cardinal neuron types
- ```{r}
- # dI6
- FeaturePlot(INs, features = c("lhx1", "dmrt3", "lbx1", "dbx2"))
- # V0
- FeaturePlot(INs, features = c("evx1", "lhx1", "nrn1", "dbx1", "cbln2"))
- # V1
- FeaturePlot(INs, features = c("pax2", "lhx1", "pnoc", "sp9", "foxd3", "en1", "gbx2", "otp"))
- # KA'
- FeaturePlot(INs, features = c("pkd1l2", "th", "tal1", "foxa2", "nkx6-2", "ptchd", "chgb", "sox1"))
- # KA''
- FeaturePlot(INs, features = c("pkd1l2", "cdx4", "cfap210", "sall2", "daw1", "tal1", "sox1", "kctd8"))
- # V2A
- FeaturePlot(INs, features = c("vsx2", "nrn1", "nkx6-2", "lhx3", "sox21", "sox14", "prdm8", "chat"))
- # V2B
- FeaturePlot(INs, features = c("gata2", "gata3" ,"lhx1", "sox1", "tal1", "slc6a5"))
- # mystery clusters
- FeaturePlot(INs, features = c("megf11", "pax2", "foxp2", "slc6a5"))
- FeaturePlot(INs, features = c("sim1"))
- ```
- Labelling clusters
- ```{r}
- DimPlot(INs, group.by = "seurat_clusters")
- [email hidden]$INs_names <- as.character([email hidden]$seurat_clusters)
- [email hidden]$INs_names =
- dplyr::recode([email hidden]$INs_names,
- "0" = "dI6 Glycinergic Interneurons",
- "1" = "V0 Glutamatergic Interneurons",
- "2" = "V0 Glutamatergic Interneurons",
- "3" = "V1 GABAergic/Glycinergic Interneurons",
- "4" = "V2a/b Glutamatergic/Cholinergic Interneurons",
- "5" = "V0 Glutamatergic Interneurons",
- "6" = "KA'' GABAergic Interneurons",
- "7" = "Differentiating Interneurons",
- "8" = "Intermediate KA Interneurons",
- "9" = "Differentiating Interneurons",
- "10" = "KA'' GABAergic Interneurons",
- "11" = "V2b/s GABAergic/Glycinergic Interneurons",
- "12" = "V0 Glutamatergic Interneurons",
- "13" = "Differentiating Interneurons",
- "14" = "KA' GABAergic/Dopaminergic Interneurons",
- "15" = "KA'' GABAergic Interneurons",
- "16" = "V0 Glutamatergic Interneurons",
- "17" = "V0 Glutamatergic Interneurons",
- "18" = "V2a Glutamatergic/Cholinergic Interneurons",
- "19" = "KA'' GABAergic Interneurons",
- "20" = "unknown Glycinergic Interneurons",
- "21" = "Differentiating Interneurons",
- "22" = "V2b GABAergic/Glycinergic Interneurons",
- "23" = "V0 Glutamatergic Interneurons")
- [email hidden]$INs_names <- factor([email hidden]$INs_names, levels = c(
- "Differentiating Interneurons",
- "V0 Glutamatergic Interneurons",
- "V1 GABAergic/Glycinergic Interneurons",
- "unknown Glycinergic Interneurons",
- "dI6 Glycinergic Interneurons",
- "V2a Glutamatergic/Cholinergic Interneurons",
- "V2b GABAergic/Glycinergic Interneurons",
- "KA'' GABAergic Interneurons",
- "Intermediate KA Interneurons",
- "KA' GABAergic/Dopaminergic Interneurons"))
- DimPlot(INs, group.by = "INs_names")
- DimPlot(INs, group.by = "INs_names", split.by = "Sample")
- ```
- Some of the mystery cells between dI6 and V1 won't cluster, so let's select them instead
- ```{r}
- unique([email hidden]$INs_names)
- barcodes <- xSelectCells::xSelectCells(INs)
- INs$cells_of_interest <- ifelse(rownames([email hidden]) %in% barcodes, "yes", "not")
- [email hidden] <- [email hidden] %>% mutate(info=paste(INs_names, cells_of_interest, sep = "_"))
- [email hidden]$INs_names =
- dplyr::recode([email hidden]$info,
- "KA'' GABAergic Interneurons_not" = "KA'' GABAergic Interneurons",
- "V1 GABAergic/Glycinergic Interneurons_not" = "V1 GABAergic/Glycinergic Interneurons",
- "V0 Glutamatergic Interneurons_not" = "V0 Glutamatergic Interneurons",
- "V2a Glutamatergic/Cholinergic Interneurons_not" = "V2a Glutamatergic/Cholinergic Interneurons",
- "dI6 Glycinergic Interneurons_not" = "dI6 Glycinergic Interneurons",
- "V2b GABAergic/Glycinergic Interneurons_not" = "V2b GABAergic/Glycinergic Interneurons",
- "KA' GABAergic/Dopaminergic Interneurons_not" = "KA' GABAergic/Dopaminergic Interneurons",
- "Intermediate KA Interneurons_not" = "Intermediate KA Interneurons",
- "unknown Glycinergic Interneurons_not" = "unknown Glycinergic Interneurons",
- "unknown Glycinergic Interneurons_yes" = "unknown Glycinergic Interneurons",
- "V1 GABAergic/Glycinergic Interneurons_yes" = "unknown Glycinergic Interneurons",
- "dI6 Glycinergic Interneurons_yes" = "unknown Glycinergic Interneurons",
- "Differentiating Interneurons_not" = "Differentiating Interneurons")
- unique([email hidden]$INs_names)
- DimPlot(INs, group.by = "INs_names")
- [email hidden]$INs_names <- factor([email hidden]$INs_names, levels = c(
- "Differentiating Interneurons",
- "dI6 Glycinergic Interneurons",
- "Regenerating V0 Glutamatergic Interneurons",
- "V0 Glutamatergic Interneurons",
- "V1 GABAergic/Glycinergic Interneurons",
- "V2a Glutamatergic/Cholinergic Interneurons",
- "Regenerating V2a Glutamatergic/Cholinergic Interneurons",
- "V2b GABAergic/Glycinergic Interneurons",
- "KA' GABAergic/Dopaminergic Interneurons",
- "KA'' GABAergic Interneurons",
- "Intermediate KA Interneurons",
- "unknown Glycinergic Interneurons"))
- saveRDS(INs, file = "INs.rds")
- ```
- Double check markers
- ```{r}
- markers <- FindAllMarkers(INs, only.pos = TRUE, group.by = "INs_names")
- markers %>%
- group_by(cluster) %>%
- dplyr::filter(avg_log2FC > 1) %>%
- slice_head(n = 3) %>%
- ungroup() -> top3
- ```
- Figures 2D, E
- ```{r}
- INspalette <- c("KA'' GABAergic Interneurons" = "steelblue1",
- "Differentiating Interneurons" = "grey",
- "V1 GABAergic/Glycinergic Interneurons" = "mediumturquoise",
- "V0 Glutamatergic Interneurons" = "coral1",
- "Regenerating V0 Glutamatergic Interneurons" = "coral1",
- "dI6 Glycinergic Interneurons" = "#FFD62B",
- "V2a Glutamatergic/Cholinergic Interneurons" = "palevioletred1",
- "Regenerating V2a Glutamatergic/Cholinergic Interneurons" = "palevioletred1",
- "V2b GABAergic/Glycinergic Interneurons" = "olivedrab3",
- "KA' GABAergic/Dopaminergic Interneurons" = "dodgerblue4",
- "Intermediate KA Interneurons" = "dodgerblue3",
- "unknown Glycinergic Interneurons" = "sienna")
- # make a heatmap for markers
- DoHeatmap(INs, features = top3$gene, group.by = "INs_names", group.colors = INspalette, size = 3, draw.lines = FALSE) + scale_fill_gradientn(colors = c("grey", "white", "navyblue")) + theme(text=element_text(size=7), plot.margin = margin(0.5, 0.75, 0, 0, "in"), legend.position="none")
- ggsave("2D.png", width = 4.5, height = 4.5, units = c("in"), dpi = 300)
- # make a umap
- DimPlot(INs, group.by = "INs_names", label = FALSE, label.size = 3, repel = TRUE, cols = INspalette, alpha = 1) + theme(axis.ticks=element_blank(), axis.text=element_blank(), plot.title=element_blank(), axis.line = element_blank(), axis.title = element_blank()) +
- scale_y_continuous(limits = c(-8, 9)) + scale_x_continuous(limits = c(-17, 10)) +
- NoLegend() +
- annotate("segment",
- x = -17, xend = -17 + c(3, 0),
- y = -8, yend = -8 + c(0, 3),
- arrow = arrow(type = "closed", length = unit(10, 'pt')))
- ggsave("2E.png", width = 5.5, height = 3.5, units = c("in"), dpi = 300)
- ```
- #Figure 2F KA neurons
- ```{r}
- KAs <- subset(x = INs, subset = ([email hidden]$INs_names == "KA'' GABAergic Interneurons" |
- [email hidden]$INs_names == "KA' GABAergic/Dopaminergic Interneurons" |
- [email hidden]$INs_names == "Intermediate KA Interneurons"))
- DotPlot_scCustom(KAs,
- features = c("gad2", "pkd1l2", "tal1", "nkx6-2", "foxa2", "ptchd", "th", "slc6a2", "slc6a4", "tph2", "slc6a3"),
- group.by = "INs_names",
- colors_use = viridis_light_high) +
- coord_flip()
- ggsave("2F.png", width = 3.5, height = 3.5, units = c("in"), dpi = 300)
- ```
Interneurons_Fig2D,2E.Rmd at commit e121c96, no license · at the source
Overview
- Program in Molecular and Cellular Biology, University of Washington,Seattle, WA USA
- Department of Biochemistry, University of Washington School of Medicine,Seattle, WA USA
- Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago,Chicago, IL USA
- Program in Molecular and Cell Biology, University of California,Berkeley, CA USA
Abstract
The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.
Repositories
Its files are read in the Code ↔ Paper reader above, with 17 matches between paragraphs and lines of code.
angellswearera/scRNAseq_xenopus_regen_wills
e121c96bec8aa44c78068cdcfb122a7b328a2e74, 4 June 2026Availability: 1 check, the latest on 27 September 2026: the link answers
- 27 September 2026: the link answers
20 files
- Fig1B,1C,1D,S2,S3.Rmd, R, 281 lines, 2 matches
- Fig2A,2B,2C,3A,S3A.Rmd, R, 250 lines, 2 matches
- Fig4C,4D,4E.Rmd, R, 453 lines
- Fig5A,5B,5C,5D,S5.Rmd, R, 512 lines
- Fig6A,6B,6C,6E,6F, S7, S10.Rmd, R, 666 lines, 2 matches
- FigS8_F-K.Rmd, R, 230 lines, 2 matches
- Figure_S11A.Rmd, R, 173 lines
- Figure_S8A-E.Rmd, R, 184 lines, 1 match
- Figure_S8A-E_initial_pro
cessing.Rmd , R, 194 lines - Interneurons_Fig2D,2E.Rm
d , R, 218 lines, 2 matches - Motor_Neurons_final.Rmd, R, 102 lines
- Neuron_neurotransmitters
.Rmd , R, 162 lines, 1 match - Regeneration_subclusters
_processing_final.Rmd , R, 276 lines - Seurat_neural_QC_final.R
md , R, 168 lines - Seurat_neural_clusters_f
inal.Rmd , R, 114 lines - Seurat_tail_QC_final.Rmd
, R, 345 lines, 2 matches - Seurat_tail_annotations_
final.Rmd , R, 204 lines, 1 match - Seurat_tail_cell_cycle_s
coring_final.Rmd , R, 37 lines, 2 matches - pseudotime.Rmd, R, 115 lines
- README.md, Text, 2 lines
Zenodo 20549290
Availability: 1 check, the latest on 27 September 2026: the link answers (HTTP 200)
- 27 September 2026: the link answers (HTTP 200)
20 files
- Fig1B,1C,1D,S2,S3.Rmd, R, 281 lines
- Fig2A,2B,2C,3A,S3A.Rmd, R, 250 lines
- Fig4C,4D,4E.Rmd, R, 453 lines
- Fig5A,5B,5C,5D,S5.Rmd, R, 512 lines
- Fig6A,6B,6C,6E,6F, S7, S10.Rmd, R, 666 lines
- FigS8_F-K.Rmd, R, 230 lines
- Figure_S11A.Rmd, R, 173 lines
- Figure_S8A-E.Rmd, R, 184 lines
- Figure_S8A-E_initial_pro
cessing.Rmd , R, 194 lines - Interneurons_Fig2D,2E.Rm
d , R, 218 lines - Motor_Neurons_final.Rmd, R, 102 lines
- Neuron_neurotransmitters
.Rmd , R, 162 lines - Regeneration_subclusters
_processing_final.Rmd , R, 276 lines - Seurat_neural_QC_final.R
md , R, 168 lines - Seurat_neural_clusters_f
inal.Rmd , R, 114 lines - Seurat_tail_QC_final.Rmd
, R, 345 lines - Seurat_tail_annotations_
final.Rmd , R, 204 lines - Seurat_tail_cell_cycle_s
coring_final.Rmd , R, 37 lines - pseudotime.Rmd, R, 115 lines
- README.md, Text, 2 lines
Code availability statement
The paper has a code availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:
- it points to the authors' code: angellswearera/
scRNAseq_xenopus_regen_w , Zenodo 20549290ills
Read it in the paper: doi.org/10.1038/s41467-026-75273-5.
Tracing map
Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.
What the map holds:
- 2 repositories of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
- 38 scripts, each with its path and the digest of its content;
- 17 matches between paragraphs of the paper and lines of the code (method lexical-v1);
- neither the text of the paper nor the code itself.
Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.
Data
Datasets cited
- bioproject:PRJNA1448102, at NCBI BioProject; found in “Data availability”
- geo:GSE327206, at NCBI GEO; found in “Data availability”
Data availability statement
The paper has a data availability statement. Its license (CC BY-NC-ND) does not allow reproducing it here; in short, from what the harvester recognized in it:
- it points to 2 datasets: NCBI BioProject PRJNA1448102, NCBI GEO GSE327206
Read it in the paper: doi.org/10.1038/s41467-026-75273-5.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 2 keywords, 9 MeSH terms, 1 funder, 114 references, 2 RRIDs.
Cite
This paper
Angell Swearer, A., Perkowski, S. B., Husain, I., Figueiredo, T. A., McCartney, M. E., & Wills, A. E. (2026). Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity. Nature communications, 17(1), 8866. https://
BibTeX
@article{angellswearer20
author = {Angell Swearer, Avery and Perkowski, Samuel B. and Husain, Iba and Figueiredo, Thiago A. and McCartney, Morgan E. and Wills, Andrea E.},
title = {{Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity}},
journal = {Nature communications},
year = {2026},
month = jul,
volume = {17},
number = {1},
pages = {8866},
publisher = {Nature Publishing Group},
issn = {2041-1723},
doi = {10.1038/
url = {https://
pmid = {42477343},
pmcid = {PMC13500724}
}
RIS
TY - JOUR
AU - Angell Swearer, Avery
AU - Perkowski, Samuel B.
AU - Husain, Iba
AU - Figueiredo, Thiago A.
AU - McCartney, Morgan E.
AU - Wills, Andrea E.
TI - Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity
T2 - Nature communications
J2 - Nat Commun
PY - 2026
DA - 2026/
VL - 17
IS - 1
SP - 8866
SN - 2041-1723
PB - Nature Publishing Group
DO - 10.1038/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1038/
"type": "article-journal",
"title": "Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity",
"container-title": "Nature communications",
"author": [
{
"family": "Angell Swearer",
"given": "Avery"
},
{
"family": "Perkowski",
"given": "Samuel B."
},
{
"family": "Husain",
"given": "Iba"
},
{
"family": "Figueiredo",
"given": "Thiago A."
},
{
"family": "McCartney",
"given": "Morgan E."
},
{
"family": "Wills",
"given": "Andrea E."
}
],
"container-title-short":
"volume": "17",
"issue": "1",
"page": "8866",
"DOI": "10.1038/
"PMID": "42477343",
"PMCID": "PMC13500724",
"ISSN": "2041-1723",
"publisher": "Nature Publishing Group",
"URL": "https://
"language": "en",
"issued": {
"date-parts": [
[
2026,
7,
20
]
]
}
}
The tracing map gets a citation of its own once an author has validated it and it has a DOI.
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