Characterization of an open-channel structure and lateral conduction pathway in the cation-selective pentameric ligand-gated ion channel, ELIC.
Overview
- Department of Anesthesiology, School of Medicine, Washington University in Saint Louis, St. Louis, MO, USA
- Laboratoire de Biochimie Théorique, Centre National de la Recherche Scientifique, Paris, France
- Center for Computational and Integrative Biology, Rutgers University, Camden, NJ, USA
- Department of Physics, Rutgers University, Camden, NJ, USA
Abstract
Open-channel structures of multiple pentameric ligand-gated ion channels (pLGICs) have been determined, including the prokaryotic model pLGIC, Erwinia ligand-gated ion channel (ELIC). For many of these structures, it remains uncertain whether they represent a physiologic open-channel state because the conditions used for structure determination do not match those of functional measurements in cell membranes. Here, MD simulation is used to examine the ion conduction properties of the ELIC open-channel structure, which was determined using a non-desensitizing mutant called ELIC5. Results from simulations show that the pore remains stably open on the microsecond timescale, but computational electrophysiology measurements demonstrate a large outward rectification and an inward conductance that is significantly lower than experiment. This discrepancy is attributed to a constricted extracellular domain (ECD), which restricts the passage of ions between the ECD vestibule and extracellular solution. Unbiased MD simulation of the ELIC5 structure demonstrates spontaneous widening of an intersubunit space in the ECD to expose a lateral fenestration, which becomes the dominant ion conduction pathway. Computational electrophysiology of the ELIC5 MD-refined structures with a widened lateral fenestration shows better agreement with experimental single-channel recordings. Mutations of residues along the lateral ion conduction pathway show reduced single-channel conductance, supporting the importance of the lateral fenestration for ion conduction in a cation-selective pLGIC.
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Data
Datasets cited
- doi:10.5061/
dryad.fxpnvx16k , at Dryad; found in “Data availability”
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Versions
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Version 2, 28 September 2026
- Publisher: n/a → Rockefeller University Press
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 6 MeSH terms, 5 funders, 67 references.
Cite
This paper
Arcario, M. J., Wu-Chen, E. J., Shim, Y., Hénin, J., Brannigan, G., & Cheng, W. W. (2026). Characterization of an open-channel structure and lateral conduction pathway in the cation-selective pentameric ligand-gated ion channel, ELIC. The Journal of general physiology, 158(5), e202513926. https://
BibTeX
@article{arcario2026char
author = {Arcario, Mark J. and Wu-Chen, Elizabeth J. and Shim, Yuna and Hénin, Jérôme and Brannigan, Grace and Cheng, Wayland W.L.},
title = {{Characterization of an open-channel structure and lateral conduction pathway in the cation-selective pentameric ligand-gated ion channel, ELIC}},
journal = {The Journal of general physiology},
year = {2026},
month = aug,
volume = {158},
number = {5},
pages = {e202513926},
publisher = {Rockefeller University Press},
issn = {0022-1295},
doi = {10.1085/
url = {https://
pmid = {42658182},
pmcid = {PMC13520879}
}
RIS
TY - JOUR
AU - Arcario, Mark J.
AU - Wu-Chen, Elizabeth J.
AU - Shim, Yuna
AU - Hénin, Jérôme
AU - Brannigan, Grace
AU - Cheng, Wayland W.L.
TI - Characterization of an open-channel structure and lateral conduction pathway in the cation-selective pentameric ligand-gated ion channel, ELIC
T2 - The Journal of general physiology
J2 - J Gen Physiol
PY - 2026
DA - 2026/
VL - 158
IS - 5
SP - e202513926
SN - 0022-1295
PB - Rockefeller University Press
DO - 10.1085/
UR - https://
LA - en
ER -
CSL-JSON
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"container-title": "The Journal of general physiology",
"author": [
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"family": "Arcario",
"given": "Mark J."
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{
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{
"family": "Shim",
"given": "Yuna"
},
{
"family": "Hénin",
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"family": "Brannigan",
"given": "Grace"
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"family": "Cheng",
"given": "Wayland W.L."
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],
"container-title-short":
"volume": "158",
"issue": "5",
"page": "e202513926",
"DOI": "10.1085/
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"ISSN": "0022-1295",
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"URL": "https://
"language": "en",
"issued": {
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