Single-cell analysis of Powassan virus-infected brains reveals age-dependent neuroinflammatory crosstalk and progressive Alzheimer's-like APP/Aβ accumulation.
Overview
- Department of Microbiology and Immunology, Center for Infectious Disease, Renaissance School of Medicine, Stony Brook University, Stony Brook, New York, USA
- SUNY Old Westbury, Old Westbury, New York, USA
- Department of Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, New York, USA
- Institut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Quebec, Canada
Abstract
Powassan virus (POWV) causes lethal encephalitis in the elderly and long-term neurological sequelae in survivors. Mirroring human disease, POWV strain LI9 directs age-dependent lethality in C57BL/
IMPORTANCE: Powassan virus (POWV) causes lethal encephalitis and long-term cognitive deficits in survivors. Using an age-dependent murine model, we reveal that POWV-infected young mice direct robust CNS inflammatory infiltrates associated with viral clearance, whereas aged mice exhibit impaired immune responses and a shift from homeostatic to neurodegenerative glial cell states. POWV prompted the induction of disease-associated microglia (DAM) and Trem2–ApoE axis transcriptional responses that are hallmarks of APP/
Reproduced under the paper's license (CC BY), from the paper cited above.
Code
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The paper's code and data availability statement is in the Data section.
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Data
Datasets cited
- geo:GSE329199, at NCBI GEO; found in “DATA AVAILABILITY”
Data availability
Complete methods underlying this study are provided in the Supplemental Methods. scRNA-seq data have been deposited in NCBI GEO under accession number GSE329199 (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 15 authors, 11 keywords, 17 MeSH terms, 3 funders, 134 references.
Cite
This paper
de Souza, M. R. M., Lindner, M. R., Mladinich-Valenti, M., Gorbunova, E. E., Kirillov, V., Byrne, A. G., Laird, A. Y., Finnerty, C. E., Ikeda, P., Vorkas, C. K., Kaur, R., Gomes, Y. C. P., Chatel-Chaix, L., Kim, H. K., & Mackow, E. R. (2026). Single-cell analysis of Powassan virus-infected brains reveals age-dependent neuroinflammatory crosstalk and progressive Alzheimer's-like APP/
BibTeX
@article{desouza2026sing
author = {de Souza, Marcos Romario Matos and Lindner, Marissa R. and Mladinich-Valenti, Megan and Gorbunova, Elena E. and Kirillov, Varvara and Byrne, Aisling G. and Laird, Autumn Y. and Finnerty, Catherine E. and Ikeda, Priscila and Vorkas, Charles K. and Kaur, Rimanpreet and Gomes, Yago C. P. and Chatel-Chaix, Laurent and Kim, Hwan Keun and Mackow, Erich R.},
title = {{Single-cell analysis of Powassan virus-infected brains reveals age-dependent neuroinflammatory crosstalk and progressive Alzheimer's-like APP/
journal = {mBio},
year = {2026},
month = jul,
volume = {17},
number = {8},
pages = {e01294--26},
publisher = {American Society for Microbiology (ASM)},
issn = {2150-7511},
doi = {10.1128/
url = {https://
pmid = {42446869},
pmcid = {PMC13464003}
}
RIS
TY - JOUR
AU - de Souza, Marcos Romario Matos
AU - Lindner, Marissa R.
AU - Mladinich-Valenti, Megan
AU - Gorbunova, Elena E.
AU - Kirillov, Varvara
AU - Byrne, Aisling G.
AU - Laird, Autumn Y.
AU - Finnerty, Catherine E.
AU - Ikeda, Priscila
AU - Vorkas, Charles K.
AU - Kaur, Rimanpreet
AU - Gomes, Yago C. P.
AU - Chatel-Chaix, Laurent
AU - Kim, Hwan Keun
AU - Mackow, Erich R.
TI - Single-cell analysis of Powassan virus-infected brains reveals age-dependent neuroinflammatory crosstalk and progressive Alzheimer's-like APP/
T2 - mBio
J2 - mBio
PY - 2026
DA - 2026/
VL - 17
IS - 8
SP - e01294
EP - 26
SN - 2150-7511
PB - American Society for Microbiology (ASM)
DO - 10.1128/
UR - https://
LA - en
ER -
CSL-JSON
{
"id": "10.1128/
"type": "article-journal",
"title": "Single-cell analysis of Powassan virus-infected brains reveals age-dependent neuroinflammatory crosstalk and progressive Alzheimer's-like APP/
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"author": [
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