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Selective hippocampal transcriptional adaptation to long-term cannabidiol exposure in mice.

Overview

Authors: J Żurowski1, E Ocłoń2,3, I Jasielczuk1, T Szmatoła1, K Mizera-Szpilka3, T Taubner4, L Szumiec5, E Semik-Gurgul6, A Gurgul1
ORCID iDs: A Gurgul
  1. Faculty of Veterinary Medicine, Department of Basic Sciences, University of Agriculture in Kraków, Redzina 1C, Krakow, 30-248 Poland
  2. Faculty of Veterinary Medicine, Laboratory of Recombinant Proteins, University of Agriculture in Kraków, Redzina 1C, Krakow, 30-248 Poland
  3. Faculty of Veterinary Medicine, Department of Infectious Disease and Public Health Protection, University of Agriculture in Kraków, Redzina 1C, Krakow, 30-248 Poland
  4. Department of Nutritional Physiology and Animal Product Quality, Institute of Animal Science, Přátelství 815, Prague, 104 00 Czech Republic
  5. Department of Molecular Neuropharmacology, Maj Institute of Pharmacology of the Polish Academy of Sciences, Smętna 12, Kraków, 31-343 Poland
  6. Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, Balice, 32-083 Poland
Institutions: University of Agriculture in Krakow (Poland); Institute of Animal Science (Czechia); Maj Institute of Pharmacology (Poland); Polish Academy of Sciences (Poland); National Research Institute of Animal Production (Poland)
Journal: BMC genomics, volume 27, issue 1, article 673
Dates: received 31 March 2026; accepted 2 June 2026; published online 8 June 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1186/s12864-026-13028-8 · PMID 42260336 · PMCID PMC13471354 · OpenAlex W7163899050
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: genetics / omics (modality), mouse (organism), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Machine learning
Keywords: Cannabidiol, Hippocampus, Mitochondrial bioenergetics, RNA-seq
MeSH: Adaptation, Physiological*, Cannabidiol*, Hippocampus*, Transcription, Genetic*, Transcriptome*, Animals, Corticosterone, Gene Expression Profiling, Gene Expression Regulation, Male, Mice, Mice, Inbred C57BL, Mitochondria, Oxidative Phosphorylation (* major topic)
Topic: Cannabis and Cannabinoid Research (Pharmacology, Medicine), according to OpenAlex
Funding: National Science Center Poland (2020/39/O/NZ9/00821); Ministry of Agriculture of the Czech Republic (MZE-RO-0723)
Citations: not cited yet (Europe PMC); 47 references in the paper

Abstract

Background: CBD is widely studied for its stress-reduction and cognitive-enhancing properties, but its effects on hippocampal molecular organisation under physiological settings are unknown. Acute and long-term intraperitoneal CBD treatment at different doses was tested on hippocampus gene expression, circulating corticosterone, and behavioural performance in C57BL/6J mice.

Results: Short-term administration did not induce detectable transcriptional changes. In contrast, long-term treatment with 10 mg/kg CBD, but not lower or higher doses, resulted in significant hippocampal transcriptional remodelling. Overrepresentation analysis showed coordinated control of mitochondrial oxidative phosphorylation genes, particularly numerous respiratory chain complex I components, and purine and nucleotide metabolic pathways. Shared mitochondrial respiratory genes, not classical disease-associated effectors, enriched KEGG categories for neurodegeneration and retrograde endocannabinoid signalling. The endocrine profile showed a temporary increase in circulating corticosterone after short-term exposure, but long-term dosing decreased it. Behavioural effects were modest and limited across paradigms.

Conclusions: These results show that long-term administration of an intermediate CBD dose alters subsets of genes related to coordinated bioenergetic and nucleotide-related transcriptional adaptation in the hippocampus, which modulates endocrine stress markers but does not disrupt behaviour. The data suggest that chronic CBD exposure may cause metabolic recalibration in stress-sensitive brain circuits rather than acute neuromolecular reprogramming.

Supplementary Information: The online version contains supplementary material available at 10.1186/s12864-026-13028-8.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

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Data

Datasets cited

Data availability

Raw sequencing reads were deposited in the Gene Expression Omnibus (GEO) NCBI database, under accession number GSE276463 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE276463) and Sort Read Archive (SRA) database with BioProject number PRJNA1157422.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 9 authors, 4 keywords, 14 MeSH terms, 2 funders, 45 references.

Cite

This paper

Żurowski, J., Ocłoń, E., Jasielczuk, I., Szmatoła, T., Mizera-Szpilka, K., Taubner, T., Szumiec, L., Semik-Gurgul, E., & Gurgul, A. (2026). Selective hippocampal transcriptional adaptation to long-term cannabidiol exposure in mice. BMC genomics, 27(1), 673. https://doi.org/10.1186/s12864-026-13028-8

BibTeX

@article{zurowski2026selective,
author = {Żurowski, J and Ocłoń, E and Jasielczuk, I and Szmatoła, T and Mizera-Szpilka, K and Taubner, T and Szumiec, L and Semik-Gurgul, E and Gurgul, A},
title = {{Selective hippocampal transcriptional adaptation to long-term cannabidiol exposure in mice}},
journal = {BMC genomics},
year = {2026},
month = jun,
volume = {27},
number = {1},
pages = {673},
publisher = {BMC},
issn = {1471-2164},
doi = {10.1186/s12864-026-13028-8},
url = {https://doi.org/10.1186/s12864-026-13028-8},
pmid = {42260336},
pmcid = {PMC13471354}
}

RIS

TY - JOUR
AU - Żurowski, J
AU - Ocłoń, E
AU - Jasielczuk, I
AU - Szmatoła, T
AU - Mizera-Szpilka, K
AU - Taubner, T
AU - Szumiec, L
AU - Semik-Gurgul, E
AU - Gurgul, A
TI - Selective hippocampal transcriptional adaptation to long-term cannabidiol exposure in mice
T2 - BMC genomics
J2 - BMC Genomics
PY - 2026
DA - 2026/06/08
VL - 27
IS - 1
SP - 673
SN - 1471-2164
PB - BMC
DO - 10.1186/s12864-026-13028-8
UR - https://doi.org/10.1186/s12864-026-13028-8
LA - en
ER -

CSL-JSON

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