Dissecting acute neuronal responses to glioblastoma using a dual-interface human iPSC neuronal culture platform.
Overview
- Department of Cancer Biology and Genetics, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210 USA
- Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH 43210 USA
- The Ohio State Biochemistry Program (OSBP), The Ohio State University, Columbus, OH 43210 USA
- Campus Chemical Instrument Center, Mass Spectrometry and Proteomics, The Ohio State University, Columbus, OH 43210 USA
- Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210 USA
- Department of Neurology, College of Medicine, The Ohio State University Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH 43210 USA
- Solove Research Institute Comprehensive Cancer Center, College of Medicine, James Cancer Hospital, The Ohio State University Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH 43210 USA
Abstract
Glioblastoma (GB) hijacks neuronal circuits to promote tumor progression, but the earliest neuronal responses remain poorly defined. We developed a dual-interface human iPSC-derived neuronal model to study acute paracrine signaling triggered by GB cells from two sources: serum-adapted U-87MG and serum-free NU-757. Within 24 h, exposed neurons displayed synaptic remodeling and activation of GB-related signaling cascades. Neurons exposed to U-87MG showed decreased dendritic spine number alongside increased total ERK and phospho-p38α at spines (1). In the soma, total ERK accumulated in the nucleus while phospho-ERK was primarily cytoplasmic; nuclear p38 and cytoplasmic MLK2 also increased (2). Conversely, NU-757 exposure enhanced spine growth but reduced postsynaptic density, NMDAR, and synaptophysin levels (3). Both total and phospho-ERK showed increased nuclear localization with NU-757, while total MLK2 and p38α levels remained stable but exhibited elevated nuclear (4) and spine localization. Pharmacological MEK/
Supplementary Information: The online version contains supplementary material available at 10.1186/
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
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- figshare:32989165, at figshare; found in DataCite
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Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 10 authors, 8 keywords, 7 MeSH terms, 6 funders, 40 references, 1 RRID.
Cite
This paper
Nebie, O., Adelakun, N., Fries, B., Kollin, L., Zhang, L., Medikonda, A., Venere, M., Giglio, P., Chu, N., & Le, N. (2026). Dissecting acute neuronal responses to glioblastoma using a dual-interface human iPSC neuronal culture platform. Acta neuropathologica communications, 14(1), 149. https://
BibTeX
@article{nebie2026dissec
author = {Nebie, Ouada and Adelakun, Niyi and Fries, Brian and Kollin, Luke and Zhang, Liwen and Medikonda, Akhil and Venere, Monica and Giglio, Pierre and Chu, Nam and Le, Nhat},
title = {{Dissecting acute neuronal responses to glioblastoma using a dual-interface human iPSC neuronal culture platform}},
journal = {Acta neuropathologica communications},
year = {2026},
month = may,
volume = {14},
number = {1},
pages = {149},
publisher = {BMC},
issn = {2051-5960},
doi = {10.1186/
url = {https://
pmid = {42141486},
pmcid = {PMC13371652}
}
RIS
TY - JOUR
AU - Nebie, Ouada
AU - Adelakun, Niyi
AU - Fries, Brian
AU - Kollin, Luke
AU - Zhang, Liwen
AU - Medikonda, Akhil
AU - Venere, Monica
AU - Giglio, Pierre
AU - Chu, Nam
AU - Le, Nhat
TI - Dissecting acute neuronal responses to glioblastoma using a dual-interface human iPSC neuronal culture platform
T2 - Acta neuropathologica communications
J2 - Acta Neuropathol Commun
PY - 2026
DA - 2026/
VL - 14
IS - 1
SP - 149
SN - 2051-5960
PB - BMC
DO - 10.1186/
UR - https://
LA - en
ER -
CSL-JSON
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