Impact of aging on neck vasculature and brain biochemistry in female mice.

Overview

1. Department of Biomedical Engineering, University of Tennessee, Knoxville, Tennessee, USA
2. Department of Psychology, University of Tennessee, Knoxville, Tennessee, USA
3. Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, USA
4. Department of Chemistry, University of Tennessee, Knoxville, Tennessee, USA
5. The Johns Hopkins University Applied Imaging Mass Spectrometry Core and Service Center, Division of Cancer Imaging Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
6. The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Abstract

Age‐related changes in large‐vessel biomechanics may contribute to neurovascular dysfunction and region‐specific biochemical remodeling in the brain, with important implications for female health. This study investigated the impact of aging on neck vasculature and brain lipid biochemistry in female C57BL/6NHsd mice by comparing young (12 weeks; n = 10) and middle‐aged (52 weeks; n = 10) cohorts using ultrasound imaging, histology, and mass spectrometry. In vivo ultrasound quantified carotid and jugular hemodynamics, including wall shear stress (WSS), circumferential cyclic strain (CCS), pulsatility index, and volumetric flow. Ex vivo assessments included spatial lipid mapping in vessels and brain, and histology staining to quantify elastin‐to‐collagen ratios. Middle‐aged females exhibited reduced carotid systolic velocity, systolic WSS, and body weight–normalized carotid volumetric flow compared to young controls, while pulsatility index and CCS showed non‐significant decreases. Histology revealed a reduction in the carotid elastin‐to‐collagen ratio, consistent with vascular remodeling. Lipidomic profiling identified age‐dependent shifts in lipid headgroups, including decreases across multiple brain lipid classes, increased N‐acylethanolamides, alongside increases in carotid cardiolipin and lysophosphatidylethanolamine. Hippocampal spectra showed clearer age‐related separation than whole‐brain analyses. Correlation analyses identified moderate to strong associations between vascular biomechanics and lipid features across tissues, supporting coordinated neurovascular and biochemical aging in female mice.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

Datasets cited

• doi:10.5061/dryad.931zcrk2n, at Dryad; found in DataCite

Data availability statement

All data associated with this study are available in the Dryad Digital Repository. Additional information is available from the corresponding author upon reasonable request.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 8 authors, 6 keywords, 9 MeSH terms, 1 funder, 74 references, 1 RRID.

Cite

This paper

Jones, A. R., Jarrahi, A., Karpowich, K., Moody, E., Renner, L., Brown, L. P., Tressler, C. M., & Crouch, A. C. (2026). Impact of aging on neck vasculature and brain biochemistry in female mice. Physiological reports, 14(17), e71059. https://doi.org/10.14814/phy2.71059

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