Triacylglycerol metabolism is a novel target to combat West Nile virus infection.
Overview
- Department of Biotechnology, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Científicas (INIA-CSIC), Madrid, Spain
- Centro de Investigación en Sanidad Animal (CISA INIA-CSIC), Valdeolmos, Spain
- State Key Laboratory of Virology and Biosafety, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of China
- Instituto de Quimica Medica (IQM, CSIC), Madrid, Spain
Abstract
West Nile virus (WNV) is a zoonotic Orthoflavivirus transmitted by mosquitoes that is responsible for outbreaks of meningitis and encephalitis worldwide. Driven by climate change, WNV has expanded as a global public health concern, particularly in temperate regions. However, there are still no specific approved therapies, reinforcing the need for antiviral development. Previous works have documented that WNV multiplication strictly depends on certain cellular lipids. To identify novel lipid-related therapeutic targets, we analyzed the infection-driven alterations in the CNS lipidome, the primary tissue supporting WNV replication. Our results indicated that the major alterations in the brain lipid content of WNV-infected mice corresponded to triacylglycerols (TAGs). Moreover, transcriptomic analysis showed that infected brains underwent changes in the expression of TAG metabolism. Supplementation with exogenous fatty acids increased lipid droplets (LD) content and promoted viral replication in cell culture models. On the contrary, pharmacological intervention in TAG metabolism using diacylglycerol acyltransferase inhibitors (DGATi) suppressed WNV multiplication in cell culture models. As a proof-of-concept of the therapeutic potential of DGATi, treatment of mice with A922500 reduced viral burden in the brain and proinflammatory cytokine production. Overall, our results unveil the importance of LDs and glycerolipid metabolism for WNV and highlight the potential of therapeutic interventions targeting this pathway to control viral replication and neuroinflammation.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- figshare:33406408, at figshare; found in DataCite
Availability of data and materials
The dataset supporting the conclusions of this article is available in the Gene Expression Omnibus repository under accession GSE310714.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 11 authors, 5 keywords, 15 MeSH terms, 1 funder, 58 references.
Cite
This paper
Caridi, F., Mingo-Casas, P., Esteban, A., Poderoso, T., de Oya, N. J., Calvo-Pinilla, E., Zhang, B., Priego, E.-M., Pérez-Pérez, M.-J., Blázquez, A.-B., & Martín-Acebes, M. A. (2026). Triacylglycerol metabolism is a novel target to combat West Nile virus infection. Emerging microbes & infections, 15(1), 2728216. https://
BibTeX
@article{caridi2026triac
author = {Caridi, Flavia and Mingo-Casas, Patricia and Esteban, Ana and Poderoso, Teresa and de Oya, Nereida Jiménez and Calvo-Pinilla, Eva and Zhang, Bo and Priego, Eva-María and Pérez-Pérez, María-Jesús and Blázquez, Ana-Belén and Martín-Acebes, Miguel A.},
title = {{Triacylglycerol metabolism is a novel target to combat West Nile virus infection}},
journal = {Emerging microbes \& infections},
year = {2026},
month = sep,
volume = {15},
number = {1},
pages = {2728216},
publisher = {Taylor \& Francis},
issn = {2222-1751},
doi = {10.1080/
url = {https://
pmid = {42678221},
pmcid = {PMC13591993}
}
RIS
TY - JOUR
AU - Caridi, Flavia
AU - Mingo-Casas, Patricia
AU - Esteban, Ana
AU - Poderoso, Teresa
AU - de Oya, Nereida Jiménez
AU - Calvo-Pinilla, Eva
AU - Zhang, Bo
AU - Priego, Eva-María
AU - Pérez-Pérez, María-Jesús
AU - Blázquez, Ana-Belén
AU - Martín-Acebes, Miguel A.
TI - Triacylglycerol metabolism is a novel target to combat West Nile virus infection
T2 - Emerging microbes & infections
J2 - Emerg Microbes Infect
PY - 2026
DA - 2026/
VL - 15
IS - 1
SP - 2728216
SN - 2222-1751
PB - Taylor & Francis
DO - 10.1080/
UR - https://
LA - en
ER -
CSL-JSON
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