Preliminary exploration of acute limit toxicity testing for nicotinamide mononucleotide in the neonatal population.
Overview
- School of Public Health, Ningxia Medical University, Yinchuan, Ningxia, China
- School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China
- The First Clinical Medical College, Ningxia Medical University, Yinchuan, Ningxia, China
- People’s Hospital of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, China
- Key Laboratory of Cerebrocranial Disease, Ningxia Medical University, Yinchuan, Ningxia, China
- School of Information Engineering, Ningxia University, Yinchuan, China
Abstract
Introduction: This study investigated the potential toxicity of nicotinamide mononucleotide (NMN) during the neonatal period, a critical and vulnerable developmental window. While NMN, a precursor of NAD+, has demonstrated safety and therapeutic potential in adults, its toxicological effects on newborns remain unclear.
Methods: We conducted an acute limit toxicity test to evaluate the impact of NMN on vital organs (liver, kidney, and central nervous system) in neonatal mice.
Results: Under these experimental conditions, no significant acute toxicity was observed, with no histopathological damage or serum biochemical abnormalities detected in the major organs. However, high-throughput transcriptome sequencing revealed subtle yet specific gene expression perturbations, involving liver proliferation signal regulation, kidney adaptation to excretory load, and brain suppression of neuroinflammation alongside coordinated growth and metabolism.
Discussion: Despite the absence of overt histopathological or clinical toxicity, transcriptomic analysis revealed that a single high-dose NMN exposure may induce early molecular-level stress and compensatory adjustments in neonates. These findings provide crucial preliminary experimental evidence and transcriptomic insights for assessing NMN safety in early life, highlighting the need for future studies on long-term, subchronic, and chronic effects, while noting that the results cannot be directly extrapolated to human infants.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- bioproject:PRJNA1461357, at NCBI BioProject; found in “Data availability statement”
Data availability statement
The data presented in the study are deposited in the SRA repository, accession number PRJNA1461357 (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 10 authors, 5 keywords, 59 references.
Cite
This paper
Cao, H., Ge, X., Cui, M., Bao, L., Ma, L., Gu, J., Xin, R., Li, G., Bi, L., & Bi, F. (2026). Preliminary exploration of acute limit toxicity testing for nicotinamide mononucleotide in the neonatal population. Frontiers in pharmacology, 17, 1773471. https://
BibTeX
@article{cao2026prelimin
author = {Cao, Hongting and Ge, Xinyu and Cui, Mingxuan and Bao, Li and Ma, Lijun and Gu, Jinhai and Xin, Ruijuan and Li, Guanghua and Bi, Li and Bi, Fengchen},
title = {{Preliminary exploration of acute limit toxicity testing for nicotinamide mononucleotide in the neonatal population}},
journal = {Frontiers in pharmacology},
year = {2026},
month = may,
volume = {17},
pages = {1773471},
publisher = {Frontiers Media SA},
issn = {1663-9812},
doi = {10.3389/
url = {https://
pmid = {42292811},
pmcid = {PMC13254182}
}
RIS
TY - JOUR
AU - Cao, Hongting
AU - Ge, Xinyu
AU - Cui, Mingxuan
AU - Bao, Li
AU - Ma, Lijun
AU - Gu, Jinhai
AU - Xin, Ruijuan
AU - Li, Guanghua
AU - Bi, Li
AU - Bi, Fengchen
TI - Preliminary exploration of acute limit toxicity testing for nicotinamide mononucleotide in the neonatal population
T2 - Frontiers in pharmacology
J2 - Front Pharmacol
PY - 2026
DA - 2026/
VL - 17
SP - 1773471
SN - 1663-9812
PB - Frontiers Media SA
DO - 10.3389/
UR - https://
LA - en
ER -
CSL-JSON
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