Dynamic regulation of mRNA acetylation at synapses by spatial memory in mouse hippocampus.
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License · 21 lines · 1 KB · MIT
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Overview
- Key Laboratory of Brain Functional Genomics, Ministry of Education and Shanghai, Affiliated Mental Health Center, School of Life Science, East China Normal University Shanghai China
- Department of Neurology, Shanghai Fifth People’s Hospital, Fudan University Shanghai China
- Shanghai Changning Mental Health Center, Affiliated Mental Health Center of East China Normal University Shanghai China
- Chongqing Key Laboratory of Precision Optics, Chongqing Institute of East China Normal University Chongqing China
- NYU-ECNU Institute of Brain and Cognitive Science at NYU Shanghai Shanghai China
Abstract
Precise regulation of protein synthesis is critical for brain functions such as long-term memory, and its dysregulation is implicated in numerous memory disorders. While mRNA methylation, such as N6-methyladenosine (m6A), has been widely studied in memory, the role of mRNA acetylation remains largely unknown. N4-acetylcytidine (ac4C), the only known form of RNA acetylation in eukaryotes, promotes mRNA stability and translation. In this study, we identified the ac4C epitranscriptome in mouse hippocampal homogenates and synaptosomes through ac4C-RNA immunoprecipitation followed by next-generation sequencing (acRIP-seq). The Morris water maze was employed to induce and evaluate memory acquisition and forgetting processes. We show that synaptic ac4C levels are dynamically regulated, increasing after memory formation and returning to baseline after natural forgetting. The dynamic changes of ac4C-mRNAs regulated by memory were validated by ac4C dot-blot, liquid chromatography–tandem mass spectrometry, and acRIP-qPCR analysis. We further demonstrate that N-acetyltransferase 10, the ac4C writer, in mouse hippocampus is important for spatial memory via regulating memory-related mRNAs, proteins, and ultimately synaptic plasticity. Lastly, we generated a freely accessible website (http://
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YinDMlab/ac4CRIP-seq-analysis
1c7529b0f52bb0d0cb7806e2ad87d84b1cef9690, 17 March 2026Availability: 1 check, the latest on 30 September 2026: the link answers
- 30 September 2026: the link answers
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Reproduced under the paper's license (CC BY), from the paper cited above.
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Data availability
All data are available in the main text, supplementary or source data files. The original data of acRIP-seq is accessible via the website (http://
The following dataset was generated:
ZhouH ZhangJ ZhuZ ChenD YinD 2024Dynamic change of ac4C in mouse hippocampus during learning and memoryNCBI Gene Expression OmnibusGSE261922
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 8 authors, 6 keywords, 13 MeSH terms, 4 funders, 115 references.
Cite
This paper
Zhou, H.-Q., Zhu, Z., Zhang, J.-W., Lin, W.-P., Jin, H.-J., Ding, Y.-Y., Liu, S., & Yin, D.-M. (2026). Dynamic regulation of mRNA acetylation at synapses by spatial memory in mouse hippocampus. eLife, 14, RP108995. https://
BibTeX
@article{zhou2026dynamic
author = {Zhou, Hai-Qian and Zhu, Zhen and Zhang, Jia-Wei and Lin, Wei-Peng and Jin, Hao-JY and Ding, Yang-Yang and Liu, Shuai and Yin, Dong-Min},
title = {{Dynamic regulation of mRNA acetylation at synapses by spatial memory in mouse hippocampus}},
journal = {eLife},
year = {2026},
month = mar,
volume = {14},
pages = {RP108995},
publisher = {eLife Sciences Publications, Ltd},
issn = {2050-084X},
doi = {10.7554/
url = {https://
pmid = {41870036},
pmcid = {PMC13008358}
}
RIS
TY - JOUR
AU - Zhou, Hai-Qian
AU - Zhu, Zhen
AU - Zhang, Jia-Wei
AU - Lin, Wei-Peng
AU - Jin, Hao-JY
AU - Ding, Yang-Yang
AU - Liu, Shuai
AU - Yin, Dong-Min
TI - Dynamic regulation of mRNA acetylation at synapses by spatial memory in mouse hippocampus
T2 - eLife
J2 - eLife
PY - 2026
DA - 2026/
VL - 14
SP - RP108995
SN - 2050-084X
PB - eLife Sciences Publications, Ltd
DO - 10.7554/
UR - https://
LA - en
ER -
CSL-JSON
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