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NAT10-dependent N<sup>4</sup>-acetylcytidine reprograms R-loops and promotes cancer stem cell growth.

Overview

Authors: Xujia Wu1,2, Donghai Wang1,3,2, Suchet Taori1,2, Weichi Wu1,3,2, Deobrat Dixit4, Deguan Lv1, Steven J Mullett5,6, Stacy L Gelhaus5,6, Fanen Yuan1, Po Zhang1, Tengfei Huang1,3, Huairui Yuan1, Qiulian Wu1,3, Jeremy N Rich1,3,7,8,9
ORCID iDs: Jeremy N Rich
  1. Hillman Cancer Center, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
  2. These authors contributed equally
  3. Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA
  4. Department of Neurology and Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY, USA
  5. Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA
  6. Health Sciences Mass Spectrometry Core, University of Pittsburgh, Pittsburgh, PA, USA
  7. Department of Neurology, University of Pittsburgh, Pittsburgh, PA, USA
  8. Department of Neurology, University of North Carolina, Chapel Hill, NC, USA
  9. Lead contact
Institutions: UPMC Hillman Cancer Center (United States); University of Pittsburgh Medical Center (United States); University of North Carolina at Chapel Hill (United States); UNC Lineberger Comprehensive Cancer Center; Herbert Irving Comprehensive Cancer Center; Columbia University (United States); University of Pittsburgh (United States)
Journal: Cell reports, volume 45, issue 6, article 117408
Dates: published online 1 June 2026; in print 23 June 2026
Type: Research article · Language: English
License: CC BY-NC-ND
Identifiers: DOI 10.1016/j.celrep.2026.117408 · PMID 42228575 · PMCID PMC13589288 · OpenAlex W7163083095
Open access: gold, a free copy (OpenAlex)
Status: data only
Categories: human (organism), mouse (organism), other condition (population), cellular / molecular (subfield)
Methods: Statistics, Smoothing, state filtering, decompositions, Machine learning, Preprocessing, Connectivity
Keywords: Glioblastoma, Cancer Stem Cell, Egr1, Glioblastoma Stem Cell, Epitranscriptomics, Nat10, Cp: Neuroscience, Cp: Cancer, Ac(4)c, R-loop Remodelin
MeSH: Cytidine*, Glioblastoma*, N-Terminal Acetyltransferase E*, Neoplastic Stem Cells*, R-Loop Structures*, Animals, Brain Neoplasms, Cell Line, Tumor, Cell Proliferation, Chromatin, Early Growth Response Protein 1, Gene Expression Regulation, Neoplastic, Humans, Mice, N-Terminal Acetyltransferases, Promoter Regions, Genetic (* major topic)
Topic: Cancer, Hypoxia, and Metabolism (Cancer Research, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: Defense Health Agency Consortium (HT9425-23-1-0689); National Institutes of Health (R01CA238662, NIHS10OD032141, R01CA268634, R35CA197718, NIHS10OD023402, R01NS103434); NCI NIH HHS (R01 CA268634, R35 CA197718, R01 CA238662); NIH HHS (S10 OD032141, S10 OD023402); University of Pittsburgh; NINDS NIH HHS (R01 NS134724, R01 NS103434, R01 NS136424); Sun Yat-sen University; Wuhan University; American Cancer Society (CSCC-LEAD-22-186-01-CSCC)
Citations: cited by 2 papers (Europe PMC); 95 references in the paper
Research resources: RRID:AB_10949503, Mouse monoclonal anti-His tag RRID:AB_11232599, RRID:AB_2118010, Rabbit polyclonal anti-NAT10 RRID:AB_2148944, RRID:AB_2210206, RRID:AB_2616028, RRID:AB_2687463, Rabbit polyclonal anti-OLIG1 RRID:AB_2788135, RRID:AB_2819183, RRID:AB_2827750, Rabbit monoclonal anti-TBK1 RRID:AB_2863061, Rabbit monoclonal anti-Phospho-TBK1 RRID:AB_2863910, Rabbit monoclonal anti-Phospho-STING RRID:AB_2864054, Rabbit recombinant anti-V5 tag RRID:AB_2878059, RRID:AB_2879008, Rabbit polyclonal anti-EGR1 RRID:AB_2881272, Mouse monoclonal anti-VEGFA RRID:AB_2882171, Rabbit polyclonal anti-TAZ RRID:AB_2889852, Rabbit monoclonal anti-STING RRID:AB_3083450, Mouse monoclonal anti-Histone H3 RRID:AB_3086558, Rabbit polyclonal anti-Histone H2A.X RRID:AB_3102323, RRID:AB_3102500, RRID:AB_3105389, Rabbit polyclonal anti-PDGFA RRID:AB_3669576, Rabbit monoclonal anti-TNF-alpha (TNFα) RRID:AB_3670472, RRID:AB_3670503, Mouse monoclonal anti-WLS/GPR177 RRID:AB_3719304

Abstract

The abstract is not reproduced here: the paper's license (CC BY-NC-ND) does not allow it. Read it in the paper, at the publisher or on Europe PMC.

Code

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The paper's code and data availability statement is in the Data section.

Tracing map

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Data

Datasets cited

Code and data availability statement

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Read it in the paper: doi.org/10.1016/j.celrep.2026.117408.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Publisher: — → Cell Press
  • Authors: added Jeremy N Rich (0000-0001-7845-5302); removed Jeremy N Rich

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 14 authors, 10 keywords, 16 MeSH terms, 9 funders, 94 references, 27 RRIDs.

Cite

This paper

Wu, X., Wang, D., Taori, S., Wu, W., Dixit, D., Lv, D., Mullett, S. J., Gelhaus, S. L., Yuan, F., Zhang, P., Huang, T., Yuan, H., Wu, Q., & Rich, J. N. (2026). NAT10-dependent N<sup>4</sup>-acetylcytidine reprograms R-loops and promotes cancer stem cell growth. Cell reports, 45(6), 117408. https://doi.org/10.1016/j.celrep.2026.117408

BibTeX

@article{wu2026nat10,
author = {Wu, Xujia and Wang, Donghai and Taori, Suchet and Wu, Weichi and Dixit, Deobrat and Lv, Deguan and Mullett, Steven J and Gelhaus, Stacy L and Yuan, Fanen and Zhang, Po and Huang, Tengfei and Yuan, Huairui and Wu, Qiulian and Rich, Jeremy N},
title = {{NAT10-dependent N\<sup\>4\</sup\>-acetylcytidine reprograms R-loops and promotes cancer stem cell growth}},
journal = {Cell reports},
year = {2026},
month = jun,
volume = {45},
number = {6},
pages = {117408},
publisher = {Cell Press},
issn = {2211-1247},
doi = {10.1016/j.celrep.2026.117408},
url = {https://doi.org/10.1016/j.celrep.2026.117408},
pmid = {42228575},
pmcid = {PMC13589288}
}

RIS

TY - JOUR
AU - Wu, Xujia
AU - Wang, Donghai
AU - Taori, Suchet
AU - Wu, Weichi
AU - Dixit, Deobrat
AU - Lv, Deguan
AU - Mullett, Steven J
AU - Gelhaus, Stacy L
AU - Yuan, Fanen
AU - Zhang, Po
AU - Huang, Tengfei
AU - Yuan, Huairui
AU - Wu, Qiulian
AU - Rich, Jeremy N
TI - NAT10-dependent N<sup>4</sup>-acetylcytidine reprograms R-loops and promotes cancer stem cell growth
T2 - Cell reports
J2 - Cell Rep
PY - 2026
DA - 2026/06/01
VL - 45
IS - 6
SP - 117408
SN - 2211-1247
PB - Cell Press
DO - 10.1016/j.celrep.2026.117408
UR - https://doi.org/10.1016/j.celrep.2026.117408
LA - en
ER -

CSL-JSON

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