OSCR

Inverse expression of Ten3 and Lphn2 across the developing mouse brain suggests a global strategy for circuit assembly.

Overview

Authors: URee Chon1,2,3, Daniel T. Pederick1,3,4, Jun Ho Song1, Yanbo Zhang1, Isabella Rana1,5, Liqun Luo1
ORCID iDs: URee Chon, Daniel T. Pederick, Liqun Luo
  1. Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
  2. Neurosciences PhD Program, Stanford University, Stanford, CA 94305, USA
  3. These authors contributed equally to this study
  4. Present address: Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
  5. Present address: Neuroscience PhD Program, Columbia University, New York, NY 10032, USA
Institutions: Howard Hughes Medical Institute (United States); Stanford University (United States)
Journal: Current biology : CB, volume 36, issue 10, pages 2606-2621.e5
Dates: published online 8 May 2026; in print 18 May 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1016/j.cub.2026.04.037 · PMID 42105756 · PMCID PMC13255788 · OpenAlex W7160649741
Open access: green, a free copy (OpenAlex)
Status: code on request
Categories: mouse (organism), cellular / molecular (subfield)
Methods: Connectivity, Statistics, Evoked potentials, fMRI & imaging, Machine learning
Keywords: Attraction, Repulsion, Target Selection, Whole Brain, Teneurin, Wiring Specificity, Heterophilic, Cell-surface Protein, Homophilic, Latrophilin
MeSH: Brain*, Gene Expression Regulation, Developmental*, Nerve Tissue Proteins*, Receptors, G-Protein-Coupled*, Receptors, Peptide*, Animals, Mice (* major topic)
Topic: Developmental Biology and Gene Regulation (Molecular Biology, Biochemistry, Genetics and Molecular Biology), according to OpenAlex
Funding: American Australian Association; Howard Hughes Medical Institute; Simons Foundation; Simons Foundation Autism Research Initiative; National Institute of Neurological Disorders and Stroke (R01-NS050580); National Institutes of Health; NINDS NIH HHS (R01 NS050835)
Citations: cited by 3 papers (Europe PMC); 79 references in the paper
Research resources: rabbit anti-HA RRID:AB_1549585, Mouse: En1tm2(cre)Wrst/J RRID:IMSR_JAX:007916, Mouse: B6;129S6-Adgrl2tm1Sud/J RRID:IMSR_JAX:023401, Mouse: B6.Cg-Slc32a1tm1.1(flpo)Hze/J RRID:IMSR_JAX:029591, Mouse: B6;129S-Slc17a6tm1.1(flpo)Hze/J RRID:IMSR_JAX:030212, Mouse: B6;129S6-Slc17a7em1(flpo)Tasic/J RRID:IMSR_JAX:034422, MATLAB RRID:SCR_001622, R RRID:SCR_001905, Fiji RRID:SCR_002285, Prism 10 RRID:SCR_002798, Imaris RRID:SCR_007370, Illustrator RRID:SCR_010279, Zen RRID:SCR_013672, ImSpector RRID:SCR_015249

Abstract

Precise wiring of neural circuits requires molecular strategies that ensure accurate target selection across diverse brain regions. Here, we identify inverse expression between a ligand–receptor pair, Teneurin-3 (Ten3) and Latrophilin-2 (Lphn2), across the developing mouse brain. Ten3 and Lphn2 exhibit inverse expression gradients along a retinotopic axis orthogonal to the ephrin-A and EphA gradients; along the tonotopic axis across multiple brainstem auditory nuclei; and along the dorsomedial–ventrolateral axis in striatum and pallidum. Their inverse expression also creates discrete domains of cerebellar Purkinje cells and cerebellar nuclei. Using conditional tag mice, we show that inverse Ten3 and Lphn2 expression patterns predict connectivity, following a ‘Ten3→Ten3, Lphn2→Lphn2’ rule in all above circuits, and that Lphn2 is required in executing this rule in Purkinje cells→cerebellar nuclei projection. Our findings suggest a global strategy of coordinating gene expression of key wiring molecules with circuit connectivity across the developing brain.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

The paper says that its authors' code is available on request: it was not published with the paper, so there is nothing to verify.

The paper's code and data availability statement is in the Data section.

Tracing map

A tracing map links a paper to the code its authors published: this paper has none (its code is available on request), so it has no map.

Data

No dataset and no data link were found in the paper.

Data and code availability

Interactive platform (Extended Data 1. Interactive visualization of Ten3 and Lphn2 expression in horizonal sections of E17 and P2 mouse brains, related to Figure 1) will be deposited at the Stanford Digital Repository and will be publicly available as the date of publication at https://doi.org/10.25740/jp473xf0650. To visualize the datasets, download all files. To visualize E17 data, open the file Ten3_Lphn2_E17.tif in Fiji by dragging it into the Fiji console. Next, launch the ROI manager in Fiji (Analyze > Tools > ROI Manager) and load the file E17_ROIs.zip. Each ROI corresponds to a defined region of interest: clicking on the ROI names will allow you to view Ten3 and Lphn2 expression across respective regions. Same for P2. Ten3 is in cyan and Lphn2 is in yellow.

This paper does not report original code.

Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 2, 28 September 2026

  • Publisher: n/a → Elsevier BV

Version 1, 28 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 10 keywords, 7 MeSH terms, 7 funders, 76 references, 14 RRIDs.

Cite

This paper

Chon, U., Pederick, D. T., Song, J. H., Zhang, Y., Rana, I., & Luo, L. (2026). Inverse expression of Ten3 and Lphn2 across the developing mouse brain suggests a global strategy for circuit assembly. Current biology : CB, 36(10), 2606-2621.e5. https://doi.org/10.1016/j.cub.2026.04.037

BibTeX

@article{chon2026inverse,
author = {Chon, URee and Pederick, Daniel T. and Song, Jun Ho and Zhang, Yanbo and Rana, Isabella and Luo, Liqun},
title = {{Inverse expression of Ten3 and Lphn2 across the developing mouse brain suggests a global strategy for circuit assembly}},
journal = {Current biology : CB},
year = {2026},
month = may,
volume = {36},
number = {10},
pages = {2606--2621.e5},
publisher = {Elsevier BV},
issn = {0960-9822},
doi = {10.1016/j.cub.2026.04.037},
url = {https://doi.org/10.1016/j.cub.2026.04.037},
pmid = {42105756},
pmcid = {PMC13255788}
}

RIS

TY - JOUR
AU - Chon, URee
AU - Pederick, Daniel T.
AU - Song, Jun Ho
AU - Zhang, Yanbo
AU - Rana, Isabella
AU - Luo, Liqun
TI - Inverse expression of Ten3 and Lphn2 across the developing mouse brain suggests a global strategy for circuit assembly
T2 - Current biology : CB
J2 - Curr Biol
PY - 2026
DA - 2026/05/08
VL - 36
IS - 10
SP - 2606
EP - 2621.e5
SN - 0960-9822
PB - Elsevier BV
DO - 10.1016/j.cub.2026.04.037
UR - https://doi.org/10.1016/j.cub.2026.04.037
LA - en
ER -

CSL-JSON

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