Deletion of Snap25 disrupts glial remodeling in aging mouse brain.
Overview
- Department of Physiology, Anatomy and Genetics, Sherrington Building, University of Oxford, Oxford, UK
- St John’s College, University of Oxford, Oxford, UK
- Department of Bioengineering, Imperial College London, London, UK
- UK Dementia Research Institute, Imperial College London, London, UK
- Department of Computing, Imperial College London, London, UK
- Chelsea and Westminster Hospital NHS Foundation Trust, London, UK
- National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, USA
- Kavli Institute for Nanoscience Discovery, Sleep and Circadian Neuroscience Institute, University of Oxford, Oxford, UK
- Sleep and Circadian Neuroscience Institute, University of Oxford, Oxford, UK
Abstract
Neuronal activity regulates glial physiology, but the effects of prolonged synaptic silencing in mature circuits are unclear. Using Rbp4-Cre-mediated Snap25 deletion to block neurotransmitter release in subsets of cortical layer 5 neurons and dentate gyrus granule cells, we examined glial responses across connected brain regions and the spinal cord in adult and middle-aged mice. Silenced cortical regions showed strong astrocytic reactivity and increased microglial density, while downstream targets, including the superior colliculus and CA3, exhibited marked microglial remodeling and synaptic changes. CA1 displayed milder alterations. In the spinal cord, microglial density decreased and GFAP+ astrocytes increased, whereas TNF-α levels and ChAT+ motor neurons were unchanged. Age amplified astrocyte heterogeneity and microglial reactivity under synaptic silencing. These findings suggest that circuit topology shapes the spatial and cellular specificity of glial responses to chronic loss of synaptic activity.
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
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Glial cell counter: https://
MicroCount code is available on request from the lead contact
Any additional information required is available from the lead contact upon request
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 2, 28 September 2026
- Authors: added Auguste Vadisiute (0000-0002-6722-7504); removed Auguste Vadisiute
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 11 authors, 1 keyword, 3 funders, 48 references.
Cite
This paper
Vadisiute, A., Szabo, F., Luchanskaya, S., Drevenakova, V., Messore, F., Ugwudike, A., Greene, G., Mueller, M., Morse, S. V., Hoerder-Suabedissen, A., & Molnár, Z. (2026). Deletion of Snap25 disrupts glial remodeling in aging mouse brain. iScience, 29(7), 116478. https://
BibTeX
@article{vadisiute2026de
author = {Vadisiute, Auguste and Szabo, Florina and Luchanskaya, Sofia and Drevenakova, Vanessa and Messore, Fernando and Ugwudike, Albert and Greene, Gretchen and Mueller, Marissa and Morse, Sophie V and Hoerder-Suabedissen, Anna and Molnár, Zoltán},
title = {{Deletion of Snap25 disrupts glial remodeling in aging mouse brain}},
journal = {iScience},
year = {2026},
month = jun,
volume = {29},
number = {7},
pages = {116478},
publisher = {Elsevier},
issn = {2589-0042},
doi = {10.1016/
url = {https://
pmid = {42382994},
pmcid = {PMC13316228}
}
RIS
TY - JOUR
AU - Vadisiute, Auguste
AU - Szabo, Florina
AU - Luchanskaya, Sofia
AU - Drevenakova, Vanessa
AU - Messore, Fernando
AU - Ugwudike, Albert
AU - Greene, Gretchen
AU - Mueller, Marissa
AU - Morse, Sophie V
AU - Hoerder-Suabedissen, Anna
AU - Molnár, Zoltán
TI - Deletion of Snap25 disrupts glial remodeling in aging mouse brain
T2 - iScience
J2 - iScience
PY - 2026
DA - 2026/
VL - 29
IS - 7
SP - 116478
SN - 2589-0042
PB - Elsevier
DO - 10.1016/
UR - https://
LA - en
ER -
CSL-JSON
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