Multimodal preclinical study of long-term effects of prenatal ultrasound on the mouse central nervous system.

Overview

1. Department of Radiology and Imaging Science, Institute of Medical Imaging, Faculty of Medicine, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
2. Gyula Petrányi Doctoral School of Clinical Immunology and Allergology, Faculty of Medicine, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
3. Doctoral School of Neuroscience, Faculty of Medicine, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
4. Department of Nuclear Medicine and Translational Imaging, Institute of Medical Imaging, Faculty of Medicine, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
5. Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
6. Department of Emergency Medicine, University of Debrecen Clinical Centre, Health Care Service Units, Clinics, University of Debrecen,Nagyerdei St. 98, Debrecen, H-4032 Hungary
7. Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas, Universidad Miguel Hernández,Sant Joan d’Alacant, Alicante, Spain
8. Department of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen,Debrecen, 4032 Hungary

Abstract

The development of the central nervous system is regulated by multiple spatio-temporally varying factors that are essential for neurons to form functional networks. Prenatal ultrasound (US) exposure may affect the dendritic arborization and spine density of CA1 neurons in the hippocampus—a key region for memory processing—but the mid- and long-term effects of this exposure are still not well understood. In this study, we used an animal model to examine how repeated prenatal US exposure impacts memory function. After treatments, we assessed the memory performance of both treated and control animals and analysed the CNS for microstructural changes in the brain tissue. We also explored potential changes in glucose metabolism and hippocampal transcriptomic profiles, focusing on calcium signaling. Our results did not reveal any changes in memory performance in US-exposed animals, despite notable microstructural impairments detected in several white matter tracts. RNA sequencing analysis revealed that US exposure causes lasting changes in calcium signaling pathways, which are known to be crucial for memory formation. Overall, our findings demonstrate that prenatal US screening in an animal model can produce enduring molecular, structural effects, underscoring the importance of further research into the safety and long-term implications of prenatal US screening.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

Datasets cited

• bioproject:PRJNA1058398, at NCBI BioProject; found in “Data availability”

Data availability

The original contributions presented in the study are included in the article/supplementary material; further inquiries can be directed to the corresponding author/s. The RNAseq raw data is available: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1058398.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 16 authors, 10 keywords, 11 MeSH terms, 1 funder, 58 references.

Cite

This paper

Karolyi, P., Kepes, Z., Pelyvas, B., Marosi, M., Juhasz, B., Berenyi, E., Englohner, A., Kocsi, E., Pelles-Tasko, B., Trouvé-Carpena, A., De Santis, S., Canals, S., Trencsenyi, G., Meszar, Z., Papp, T., & Károlyi, P. (2026). Multimodal preclinical study of long-term effects of prenatal ultrasound on the mouse central nervous system. Scientific reports, 16(1), 18549. https://doi.org/10.1038/s41598-026-49034-9

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