Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid - TGR5 pathway.
Overview
- Institute for the Animal Production System in the Mediterranean Environment, National Research Council, P.Le Enrico Fermi 1, Portici, 80055 Italy
- Department of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Edificio 7, Via Cintia, Naples, 80126 Italy
- Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Via Pansini 5, Naples, 80100 Italy
- NBFC, National Biodiversity Future Center, Palermo, 90133 Italy
- Task Force On Microbiome Studies, University of Naples Federico II, Naples, 80126 Italy
Abstract
Objective: The increased intake of added sweeteners, such as high-fructose corn syrup, has been associated with a rise in metabolic dysfunctions in gut and brain. While different evidence showed that dietary fructose induces gut microbiota reshaping, the sugar impact on specific bacteria-derived metabolites remains an understudied topic. In this study, we identified secondary bile acids (sBAs) as molecules differentially represented in plasma of rats fed a fructose-rich diet compared to control animals, and hypothesized that these metabolites might be a target for probiotic-based strategies to counteract sugar-induced metabolic disorders. To this aim, we investigated whether probiotic spores of Shouchella clausii SF174 ameliorate fructose-induced cognitive and metabolic dysfunctions and prevent molecular alterations in hippocampus and frontal cortex.
Methods: Wistar rats were fed a fructose-rich diet, alone or in combination with the daily administration of Shouchella clausii spores, for six weeks. At the end of treatment, behavioral, metabolomic and molecular analyses were performed.
Results: The probiotic spores exerted a protective effect on the memory function of fructose fed rats and prevented the decrease of markers of synaptic plasticity. This was associated with the maintenance of plasma and brain levels of the sBA deoxycholic acid and of its specific receptor Takeda G protein-coupled receptor 5. Further, spores beneficial modulation of fructose-induced peripheral and central inflammation was observed. Also, probiotic spores produced reshaping of the gut microbiota towards a composition exerting neuroprotective and anti-inflammatory effects.
Conclusion: These results suggest that sBAs might act as a communication bridge along the microbiota gut-brain axis and suggest that their modulation, through probiotic administration, represents an effective strategy to counteract fructose-induced neuroinflammation and gut-brain dysfunction.
Supplementary Information: The online version contains supplementary material available at 10.1186/
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- bioproject:PRJNA1291627, at NCBI BioProject; found in the text, “DNA extraction, high-throughput sequencing and…”
Data availability
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Reproduced under the paper's license (CC BY), from the paper cited above.
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 15 authors, 8 keywords, 13 MeSH terms, 3 funders, 84 references.
Cite
This paper
Spagnuolo, M. S., Petecca, N., De Palma, F., Troise, A. D., Di Porzio, A., Barrella, V., Saggese, A., De Pascale, S., De Stefano, M., Scaloni, A., Baccigalupi, L., Ricca, E., Iossa, S., Mazzoli, A., & Cigliano, L. (2026). Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid - TGR5 pathway. Molecular medicine (Cambridge, Mass.), 32(1), 101. https://
BibTeX
@article{spagnuolo2026gu
author = {Spagnuolo, Maria Stefania and Petecca, Natasha and De Palma, Francesca and Troise, Antonio Dario and Di Porzio, Angela and Barrella, Valentina and Saggese, Anella and De Pascale, Sabrina and De Stefano, Marina and Scaloni, Andrea and Baccigalupi, Loredana and Ricca, Ezio and Iossa, Susanna and Mazzoli, Arianna and Cigliano, Luisa},
title = {{Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid - TGR5 pathway}},
journal = {Molecular medicine (Cambridge, Mass.)},
year = {2026},
month = may,
volume = {32},
number = {1},
pages = {101},
publisher = {The Feinstein Institute for Medical Research},
issn = {1076-1551},
doi = {10.1186/
url = {https://
pmid = {42106599},
pmcid = {PMC13326143}
}
RIS
TY - JOUR
AU - Spagnuolo, Maria Stefania
AU - Petecca, Natasha
AU - De Palma, Francesca
AU - Troise, Antonio Dario
AU - Di Porzio, Angela
AU - Barrella, Valentina
AU - Saggese, Anella
AU - De Pascale, Sabrina
AU - De Stefano, Marina
AU - Scaloni, Andrea
AU - Baccigalupi, Loredana
AU - Ricca, Ezio
AU - Iossa, Susanna
AU - Mazzoli, Arianna
AU - Cigliano, Luisa
TI - Gut-brain axis: beneficial impact of Shouchella clausii spores on fructose induced dysfunction is associated with modulation of the deoxycholic acid - TGR5 pathway
T2 - Molecular medicine (Cambridge, Mass.)
J2 - Mol Med
PY - 2026
DA - 2026/
VL - 32
IS - 1
SP - 101
SN - 1076-1551
PB - The Feinstein Institute for Medical Research
DO - 10.1186/
UR - https://
LA - en
ER -
CSL-JSON
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