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The effects of blast-induced traumatic brain injury on brain cellular mechanics and differentiation.

Overview

Authors: Nabila Masud1, Catherine Fonder2,3,4, Bridget McGovern5, Md Hasibul Hasan Hasib1, William J. Jackson6, Dulce C. Resendiz6, Carley Rivers6,7, Sarah A. Bentil6, Donald S. Sakaguchi2,3,4,5, Anwesha Sarkar1
ORCID iDs: Catherine Fonder, Anwesha Sarkar
  1. Electrical and Computer Engineering, Iowa State University, Ames, Iowa, United States of America
  2. Molecular, Cellular, and Developmental Biology Program, Iowa State University, Ames, Iowa, United States of America
  3. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa, United States of America
  4. Nanovaccine Institute, Iowa State University, Ames, Iowa, United States of America
  5. Biology Program, Iowa State University, Ames, Iowa, United States of America
  6. Mechanical Engineering, Iowa State University, Ames, Iowa, United States of America
  7. Neuroscience Graduate Program, Iowa State University, Ames, Iowa, United States of America
Institutions: Iowa State University (United States)
Journal: PloS one, volume 21, issue 8, article e0355739
Dates: received 31 July 2025; accepted 26 July 2026; published online 13 August 2026
Type: Research article · Language: English
License: CC BY
Identifiers: DOI 10.1371/journal.pone.0355739 · PMID 42594130 · PMCID PMC13472447 · OpenAlex W7202362614
Open access: gold, a free copy (OpenAlex)
Status: dead link
Categories: histology / microscopy (modality), rat (organism), traumatic brain injury (population), cellular / molecular (subfield)
Methods: Spectral & time-frequency, Statistics
MeSH: Blast Injuries*, Brain Injuries, Traumatic*, Cell Differentiation*, Hippocampus*, Animals, Biomechanical Phenomena, Cell Proliferation, Cell Survival, Microscopy, Atomic Force, Neurons, Rats (* major topic)
Journal subjects: Research and Analysis Methods, Imaging Techniques, Fluorescence Imaging, Biology and Life Sciences, Cell Biology, Cellular Structures and Organelles, Cytoskeleton, Physical Sciences, Materials Science, Material Properties, Viscoelasticity, Medicine and Health Sciences, Critical Care and Emergency Medicine, Trauma Medicine, Traumatic Injury, Neurotrauma, Traumatic Brain Injury, Developmental Biology, Cell Differentiation, Neuronal Differentiation, Neurology, Brain Damage, Chemistry, Chemical Properties, Viscosity, Physical Chemistry, Materials Physics, Physics
Topic: Traumatic Brain Injury Research (Epidemiology, Medicine), according to OpenAlex
Funding: Iowa State University Harpole-Pentair Professorship (AS); Stem Cell Research Support Fund (DSS)
Citations: not cited yet (Europe PMC); 75 references in the paper

Abstract

Blast-induced traumatic brain injury (bTBI) causes significant disruptions in cellular and subcellular structures within the central nervous system (CNS) when an extremely large force is applied. The corresponding changes in biomechanical properties and cellular functionalities of neuronal and glial cells due to bTBI remain largely unexplored. In this work, high blast overpressures (BOPs) of 14.5 psi (single shockwave) and 29.0 psi (double shockwave) were applied to adult hippocampal progenitor cells (AHPCs) in two different directions (overpressure applied from ‘top-to-bottom’ and ‘bottom-to-top’ direction on the cell culture petridish). The resultant alterations in structural, nanomechanical, and viscoelastic properties as well as cellular survival, proliferation, and differentiation were analyzed using atomic force microscopy (AFM) and immunocytochemistry (ICC). Double shockwave exposure from ‘bottom-to-top’ direction yielded reduced Young’s modulus, surface roughness, and viscosity, causing significant actin cytoskeletal disruptions compared to ‘top-to-bottom’ direction. ICC results demonstrated that double shockwave exposure from ‘top-to-bottom’ direction caused populations of oligodendrocytes and immature neurons to decrease, while ‘bottom-to-top’ double shockwave exposure caused an increase in the percentage of immature neurons as shown by increased TuJ1-immunoreactivity which is interpreted as evidence that cells have committed to a neuronal lineage and entered an immature/early neuronal stage. These findings emphasize the interplay among cellular differentiation, mechanics, and resilience of neuronal and glial cells to trauma in bTBI aftermath.

Reproduced under the paper's license (CC BY), from the paper cited above.

Code

No file of the authors' code could be read here: it is described below, and read at its source.

kaggle.com/datasets/masudnabila

License: none: the authors keep all their rights
State: the link is dead, verified on 27 September 2026
Evidence: found in the paper
Software Heritage: not checked
Found in: “Data Availability”
Not found: README, license file, CITATION.cff, environment file, tests, continuous integration, documentation
Availability: 1 check, the latest on 27 September 2026: the link is dead (HTTP 404)
  • 27 September 2026: the link is dead (HTTP 404)
At the source: kaggle.com/datasets/masudnabila/btbi-datasets

The paper's code and data availability statement is in the Data section.

Tracing map

Proposed by the machine: these links were found in the paper and verified at the source, without human review. The map will receive a Zenodo DOI once one of the paper's authors has validated it with their ORCID.

What the map holds:

  • 1 repository of the authors' code, each at its verified commit, with its license and how the link was found in the paper;
  • 0 scripts, each with its path and the digest of its content;
  • no match between paragraphs and code yet;
  • neither the text of the paper nor the code itself.

Its JSON (tracing-map.json) is deposited on Zenodo with its DOI once the map is validated.

Data

No dataset and no data link were found in the paper.

Data Availability

All raw data, images, spreadsheets, and analysis scripts are available at Kaggle [https://www.kaggle.com/datasets/masudnabila/btbi-datasets].

Reproduced under the paper's license (CC BY), from the paper cited above.

Versions

The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.

Version 1, 27 September 2026: the first record

Recorded: type, language, journal, volume, issue, pages, dates, 10 authors, 11 MeSH terms, 2 funders, 71 references.

Cite

This paper

Masud, N., Fonder, C., McGovern, B., Hasib, M. H. H., Jackson, W. J., Resendiz, D. C., Rivers, C., Bentil, S. A., Sakaguchi, D. S., & Sarkar, A. (2026). The effects of blast-induced traumatic brain injury on brain cellular mechanics and differentiation. PloS one, 21(8), e0355739. https://doi.org/10.1371/journal.pone.0355739

BibTeX

@article{masud2026effects,
author = {Masud, Nabila and Fonder, Catherine and McGovern, Bridget and Hasib, Md Hasibul Hasan and Jackson, William J. and Resendiz, Dulce C. and Rivers, Carley and Bentil, Sarah A. and Sakaguchi, Donald S. and Sarkar, Anwesha},
title = {{The effects of blast-induced traumatic brain injury on brain cellular mechanics and differentiation}},
journal = {PloS one},
year = {2026},
month = aug,
volume = {21},
number = {8},
pages = {e0355739},
publisher = {PLOS},
issn = {1932-6203},
doi = {10.1371/journal.pone.0355739},
url = {https://doi.org/10.1371/journal.pone.0355739},
pmid = {42594130},
pmcid = {PMC13472447}
}

RIS

TY - JOUR
AU - Masud, Nabila
AU - Fonder, Catherine
AU - McGovern, Bridget
AU - Hasib, Md Hasibul Hasan
AU - Jackson, William J.
AU - Resendiz, Dulce C.
AU - Rivers, Carley
AU - Bentil, Sarah A.
AU - Sakaguchi, Donald S.
AU - Sarkar, Anwesha
TI - The effects of blast-induced traumatic brain injury on brain cellular mechanics and differentiation
T2 - PloS one
J2 - PLoS One
PY - 2026
DA - 2026/08/13
VL - 21
IS - 8
SP - e0355739
SN - 1932-6203
PB - PLOS
DO - 10.1371/journal.pone.0355739
UR - https://doi.org/10.1371/journal.pone.0355739
LA - en
ER -

CSL-JSON

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