Network Pharmacology-Based Elucidation of Gu's Anshen Decoction in Modulating TNF Signaling to Ameliorate Insomnia.
Overview
Abstract
Objective: To investigate the therapeutic mechanisms of Gu’s Anshen decoction (GAD) against insomnia (INS), focusing on the regulation of tumor necrosis factor (TNF)-mediated inflammatory pathways.
Methods: Bioactive compounds and potential targets of GAD were identified via network pharmacology and constructed herb–compound–target interaction network. INS-related targets derived from peripheral blood mononuclear cells in the Gene Expression Omnibus (GEO) database were intersected with GAD targets to establish a protein-protein interaction (PPI) network. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to identify the key pathways. Experimental validation employed a para-chlorophenylalanine
Results: Network pharmacology revealed multi-target, multi-pathway actions of GAD against INS, with the TNF signaling pathway identified as a critical axis. Animal experiments demonstrated that GAD significantly improved sleep architecture in INS rats, reduced serum and hippocampal levels of TNF-α and interleukin-1β (IL-1β), and downregulated phosphorylated NF-κB p65 (p-p65) expression, indicating suppression of TNF-driven neuroinflammation.
Conclusion: GAD ameliorates INS in a rat model by inhibiting TNF-mediated inflammatory signaling, offering a potential mechanistic basis for its therapeutic use in INS management.
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
Code
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Data
Datasets cited
- geo:GSE208668, at NCBI GEO; found in the text, “Functional Enrichment Analysis of INS…”
Other data links
- ncbi.nlm.nih.gov/
geo , NCBI; found in the text, “Identification and Analysis of INS-Related…”
Data Sharing Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
Versions
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Version 1, 28 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 2 authors, 5 keywords, 47 references.
Cite
This paper
Gu, S., & Xu, J. (2026). Network Pharmacology-Based Elucidation of Gu's Anshen Decoction in Modulating TNF Signaling to Ameliorate Insomnia. Nature and science of sleep, 18, 582222. https://
BibTeX
@article{gu2026network,
author = {Gu, Sanyuan and Xu, Jingyu},
title = {{Network Pharmacology-Based Elucidation of Gu's Anshen Decoction in Modulating TNF Signaling to Ameliorate Insomnia}},
journal = {Nature and science of sleep},
year = {2026},
month = may,
volume = {18},
pages = {582222},
publisher = {Dove Press},
issn = {1179-1608},
doi = {10.2147/
url = {https://
pmid = {42233047},
pmcid = {PMC13225028}
}
RIS
TY - JOUR
AU - Gu, Sanyuan
AU - Xu, Jingyu
TI - Network Pharmacology-Based Elucidation of Gu's Anshen Decoction in Modulating TNF Signaling to Ameliorate Insomnia
T2 - Nature and science of sleep
J2 - Nat Sci Sleep
PY - 2026
DA - 2026/
VL - 18
SP - 582222
SN - 1179-1608
PB - Dove Press
DO - 10.2147/
UR - https://
LA - en
ER -
CSL-JSON
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"given": "Sanyuan"
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"publisher": "Dove Press",
"URL": "https://
"language": "en",
"issued": {
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