Exploring the molecular mechanism of dexmedetomidine in alleviating blood-brain barrier disruption in rats with cerebral ischemia reperfusion injury based on network pharmacology.

Overview

1. Department of Human Anatomy, Baotou Medical College, Baotou, China
2. Department of Anesthesiology, The Third Hospital of Baogang Group, Baotou, China
3. Key Laboratory of Human Anatomy, Education Department of Inner Mongolia Autonomous Region, Baotou, China

Abstract

Objective: This study aimed to clarify the neuroprotective effect of dexmedetomidine (DEX) against cerebral ischemia reperfusion injury (CIRI) and its underlying mechanism using network pharmacology and in vivo validation.

Methods: Network pharmacology was employed to explore the mechanism underlying DEX-mediated alleviation of CIRI. A rat CIRI model was established using the suture-occlusion method. Neurological scoring and behavioral assessments were conducted to evaluate neurological and motor functions; histological examination was performed to observe brain tissue and blood–brain barrier (BBB) injury. Western blotting and immunofluorescence analysis were utilized to assess the protein levels of factors associated with BBB integrity.

Results: Network pharmacology analysis revealed that DEX may exert a protective effect against CIRI through the AMPK/mTOR signaling pathway. DEX treatment significantly attenuated CIRI-induced impairments in neurological function and motor performance. Specifically, DEX upregulated the protein expression levels of P-AMPK/AMPK ratio, beclin 1, LC3 II/I, and ZO-1, whereas the P-mTOR/mTOR ratio and P62 were significantly downregulated, and cerebral tissue injury was alleviated.

Conclusion: DEX exerts a significant protective effect against BBB injury in rats with CIRI. This neuroprotective effect is mediated by multiple synergistic mechanisms, including the upregulation of tight junction proteins and the regulation of the AMPK/mTOR signaling pathway. Collectively, the findings of the present study suggest that DEX represents a promising potential agent for the clinical treatment of CIRI-associated BBB impairment.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Data

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• doi:10.5061/dryad.q83bk3jxz, at Dryad; found in “Data availability statement”

Data availability statement

The original contributions presented in the study are publicly available. This data can be found here: https://doi.org/10.5061/dryad.q83bk3jxz.

Reproduced under the paper's license (CC BY), from the paper cited above.

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Version 1, 29 September 2026: the first record

Recorded: type, language, journal, volume, pages, dates, 4 authors, 6 keywords, 38 references.

Cite

This paper

Lv, X., Gao, W., Zhang, Z.-G., & Jia, J.-X. (2026). Exploring the molecular mechanism of dexmedetomidine in alleviating blood-brain barrier disruption in rats with cerebral ischemia reperfusion injury based on network pharmacology. Frontiers in molecular neuroscience, 19, 1750882. https://doi.org/10.3389/fnmol.2026.1750882

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