Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis.
Overview
- Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; (G.L.W.); (M.S.K.); (S.M.); (S.B.)
- Graduate School of Biomedical Sciences, Wake Forest University School of Medicine, Winston-Salem, NC 27101, USA
- The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
- Department of Cell Biology and Genetics, College of Medicine, Texas A&M University Health Science Center at Houston, Bryan, TX 77807-3260, USA
- Department of Translational Medicine, Naresh K. Vashisht College of Medicine, Texas A&M Health Science Center, Texas A&M University, Bryan, TX 77807-3260, USA
- Institute of Biosciences & Technology, Texas A&M Health Science Center, Texas A&M University, Houston, TX 77030, USA
- Department of Cell Biology and Genetics, Naresh K. Vashisht College of Medicine, Texas A&M Health Science Center, Texas A&M University, 4209 MREB II, 8447 John Sharp Parkway, Bryan, TX 77807-3260, USA
Abstract
Mechanisms underlying breast cancer brain metastasis (BCBM) are still not well understood. Here, we identified that BCBM patient serum contained extracellular vesicles (EVs) with high levels of microRNAs (miRNAs)-107 and -425. Levels of miR-107 and miR-425 were elevated in brain metastases, and the elevation was associated with poor patient prognoses. Ectopic expression of miR-107 and miR-425 promoted mammospheres; however, the inhibition of miR-425, but not miR-107, suppressed breast cancer mammosphere formation. We further observed that EVs from miR-425-overexpressing breast cancer cells strongly activated astrocytes whereas their inhibitors abrogated the effect. Conditioned media from miR-425-activated astrocytes promoted mammospheres. To elucidate how miR-425 activates astrocytes, we found that, within astrocytes, miR-425 suppressed the expression of transcription factor ZNF24, which downregulated CCL8 cytokine expression/
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- geo:GSE68373, at NCBI GEO; found in the text, “2.1. Breast Cancer EV-Derived miR-107 and…”
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors on request.
Reproduced under the paper's license (CC BY), from the paper cited above.
Versions
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Version 1, 30 September 2026: the first record
Recorded: type, language, journal, volume, issue, pages, dates, 6 authors, 5 keywords, 14 MeSH terms, 4 funders, 80 references.
Cite
This paper
Wong, G. L., Khan, M. S., Manore, S., Bindal, S., Singh, R., & Lo, H.-W. (2026). Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis. International journal of molecular sciences, 27(7), 3197. https://
BibTeX
@article{wong2026breast,
author = {Wong, Grace L and Khan, Munazza S and Manore, Sara and Bindal, Shivani and Singh, Ravi and Lo, Hui-Wen},
title = {{Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis}},
journal = {International journal of molecular sciences},
year = {2026},
month = mar,
volume = {27},
number = {7},
pages = {3197},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
issn = {1422-0067},
doi = {10.3390/
url = {https://
pmid = {41977380},
pmcid = {PMC13073527}
}
RIS
TY - JOUR
AU - Wong, Grace L
AU - Khan, Munazza S
AU - Manore, Sara
AU - Bindal, Shivani
AU - Singh, Ravi
AU - Lo, Hui-Wen
TI - Breast Cancer-Derived Extracellular Vesicle miR-425-5p (miR-425) Promotes Brain Metastasis via Activating Astrocytes Through the Novel miR-425-ZNF24-CCL8 Signaling Axis
T2 - International journal of molecular sciences
J2 - Int J Mol Sci
PY - 2026
DA - 2026/
VL - 27
IS - 7
SP - 3197
SN - 1422-0067
PB - Multidisciplinary Digital Publishing Institute (MDPI)
DO - 10.3390/
UR - https://
LA - en
ER -
CSL-JSON
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"container-title": "International journal of molecular sciences",
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