Mendelian Randomization and Single-Cell RNA Sequencing Reveal CKAP4 and PFDN5 as Tumor Cell-Specific Causal Genes for Glioblastoma.
Overview
Abstract
Background: Glioblastoma (GBM) is an aggressive primary brain tumor with unclear etiology. We aimed to identify causal risk genes by integrating single-cell RNA sequencing (scRNA-seq) and Mendelian randomization (MR).
Methods: Differentially expressed genes (DEGs) from public databases were overlapped and analyzed via MR to screen for causal genes. A prognostic model was built using these genes and validated through immune infiltration analysis, single-cell mapping, and experimental assays (RT-qPCR, Western blot).
Results: A 6-gene prognostic signature (TMEM158, HOXB2, CKAP4, PEPD, PFDN5, NPC2) was established, where higher risk scores correlated with poorer overall survival and distinct immune profiles. scRNA-seq confirmed tumor cell-specific expression, validated experimentally. Multivariate MR highlighted CKAP4 and PFDN5 as having direct causal links to GBM.
Conclusion: The 6-gene signature predicts GBM prognosis, and CKAP4/
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
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Data
Datasets cited
- geo:GSE68848 — at NCBI GEO; found in the text, “Validation of Biomarker Expression and…”
Data Sharing Statement
The data underlying this article are available in the Cancer Genome Atlas (TCGA) at http://
Reproduced under the paper's license (CC BY-NC), from the paper cited above.
Versions
The history of this record: each version stored by the harvester or made by a correction of its authors or of the maintainers of its code, and what changed in its facts. The texts of the paper (its abstract, its availability statements) are not part of it; versions that changed only those are not listed.
Version 1, 27 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 9 authors, 6 keywords, 57 references.
Cite
This paper
Huang, C., Dong, M., Yu, Y., Wang, C., Huang, C., Li, T., Su, X., Shi, F., & Feng, D. (2026). Mendelian Randomization and Single-Cell RNA Sequencing Reveal CKAP4 and PFDN5 as Tumor Cell-Specific Causal Genes for Glioblastoma. International journal of general medicine, 19, 605071. https://
BibTeX
@article{huang2026mendel
author = {Huang, Chong and Dong, Minhai and Yu, Yongjia and Wang, Chunxi and Huang, Chaojue and Li, Tang and Su, Xiangsheng and Shi, Fengqiang and Feng, Daqin},
title = {{Mendelian Randomization and Single-Cell RNA Sequencing Reveal CKAP4 and PFDN5 as Tumor Cell-Specific Causal Genes for Glioblastoma}},
journal = {International journal of general medicine},
year = {2026},
month = jun,
volume = {19},
pages = {605071},
publisher = {Dove Press},
issn = {1178-7074},
doi = {10.2147/
url = {https://
pmid = {42369563},
pmcid = {PMC13308729}
}
RIS
TY - JOUR
AU - Huang, Chong
AU - Dong, Minhai
AU - Yu, Yongjia
AU - Wang, Chunxi
AU - Huang, Chaojue
AU - Li, Tang
AU - Su, Xiangsheng
AU - Shi, Fengqiang
AU - Feng, Daqin
TI - Mendelian Randomization and Single-Cell RNA Sequencing Reveal CKAP4 and PFDN5 as Tumor Cell-Specific Causal Genes for Glioblastoma
T2 - International journal of general medicine
J2 - Int J Gen Med
PY - 2026
DA - 2026/
VL - 19
SP - 605071
SN - 1178-7074
PB - Dove Press
DO - 10.2147/
UR - https://
LA - en
ER -
CSL-JSON
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"URL": "https://
"language": "en",
"issued": {
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