<i>Laminaria japonica</i> polysaccharide mitigates acute neuroinflammation in cerebral ischemia-reperfusion injury through Csf3-modulated pathways.
Overview
- Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China
- Laboratory of Clinical Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China
- Marine Biomedical Research Institution, Guangdong Medical University, Zhanjiang, China
- School of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang, China
Abstract
Introduction: Microglial hyperactivation-driven neuroinflammation is a major driver of secondary brain injury following cerebral ischemia/
Methods: LJP was isolated from Laminaria japonica and structurally characterized via high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared (FT-IR) spectroscopy, and ion chromatography. Its neuroprotective effects were evaluated in a mouse model of transient middle cerebral artery occlusion (tMCAO), with assessments of infarct volume, neurological function, and neuroinflammatory markers. Cross-species transcriptomic analysis identified potential targets, which were validated through in vivo and in vitro functional assays (rescue experiments with recombinant Csf3 and neutralization assays with Csf3-specific antibodies).
Results: Structural characterization confirmed LJP is enriched in fucose, galacturonic acid, glucuronic acid, and sulfate groups. In tMCAO mice, LJP administration reduced infarct volume, improved neurological function, and suppressed microglial pro-inflammatory polarization, accompanied by decreased levels of interleukin-1b (IL-1b), tumor necrosis factor-α (TNFα), and interleukin-6 (IL-6). Transcriptomic analysis identified granulocyte colony-stimulating factor (Csf3/
Discussion: These findings demonstrate that LJP mitigates acute neuroinflammation post-I/
Reproduced under the paper's license (CC BY), from the paper cited above.
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Data
Datasets cited
- geo:GSE328360, at NCBI GEO; found in “Data availability statement”
Data availability statement
The original contributions presented in the study are publicly available. This data can be found at the NCBI Gene Expression Omnibus (GEO) under accession number GSE328360 (https://
Reproduced under the paper's license (CC BY), from the paper cited above.
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Version 1, 29 September 2026: the first record
Recorded: type, language, journal, volume, pages, dates, 8 authors, 5 keywords, 17 MeSH terms, 3 funders, 32 references.
Cite
This paper
Guan, T., Chen, S., Jiang, D., Cai, W., Chen, H., Wang, Y., Wu, K., & Zhou, X. (2026). &
BibTeX
@article{guan2026lt,
author = {Guan, Tangming and Chen, Siyu and Jiang, Dongbo and Cai, Weiming and Chen, Huayan and Wang, Yan and Wu, Kefeng and Zhou, Xin},
title = {{\&
journal = {Frontiers in immunology},
year = {2026},
month = apr,
volume = {17},
pages = {1801746},
publisher = {Frontiers Media SA},
issn = {1664-3224},
doi = {10.3389/
url = {https://
pmid = {42112322},
pmcid = {PMC13149078}
}
RIS
TY - JOUR
AU - Guan, Tangming
AU - Chen, Siyu
AU - Jiang, Dongbo
AU - Cai, Weiming
AU - Chen, Huayan
AU - Wang, Yan
AU - Wu, Kefeng
AU - Zhou, Xin
TI - &
T2 - Frontiers in immunology
J2 - Front Immunol
PY - 2026
DA - 2026/
VL - 17
SP - 1801746
SN - 1664-3224
PB - Frontiers Media SA
DO - 10.3389/
UR - https://
LA - en
ER -
CSL-JSON
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